FastQCFastQC Report
Fri 10 Feb 2017
ERR1631313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746159
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100391.3454236965579722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82171.1012398161785892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71470.9578387448251646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.34188423646970684No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA19360.2594621253647011No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA10900.14608146521049803No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9450.12664860974671618No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9260.1241022355824965No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA9160.1227620386539598No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.11847340848264243No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8210.11003016783286138No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8090.10842193151861734No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA8020.10748379366864168No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC7700.1031951634973243No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470.10011271056168994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCATG352.3871933E-531.71428728
GTACTAG358.866467E-426.4285721
TATAGAG358.866467E-426.4285722
CTAGATA451.3226047E-424.6666663
ATTAGAC701.9210347E-723.7857153
TAGCGTC400.001930555423.12500219
GGTATTG652.680752E-622.7692333
GCTCTAG603.7240316E-521.5833321
GGTATCA39100.021.1496161
GTACCTA705.097965E-621.1428591
TTAATGG705.097965E-621.14285915
GCATATT705.097965E-621.14285921
GGCAGTC13000.020.9192318
GACACAT1151.3460522E-1020.91304426
AGTACCG1151.3460522E-1020.9130445
CAGTCGG13100.020.90076310
GCAGTCG13050.020.8390799
AGTCGGT13200.020.60227211
AGCGTCA450.003824569520.5555553
GTATTAA450.003824569520.5555551
Produced by FastQC (version 0.11.5)