FastQCFastQC Report
Fri 10 Feb 2017
ERR1631312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20869
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT350.16771287555704634TruSeq Adapter, Index 8 (95% over 22bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA270.12937850400115003No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC240.11500311466768891No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT210.1006277253342278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC751.9474079E-417.26666637
TCTTATA1151.1057473E-616.08695637
CTCTTAT1303.962952E-614.23076936
TCTCTTA1552.4326935E-511.93548435
GTCTCTT2104.8322967E-59.69047537
TGTCTCT2656.0429226E-47.679245536
CTGTCTC3401.7042532E-57.617647637
Produced by FastQC (version 0.11.5)