##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631311.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5771
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07953560907988	33.0	31.0	34.0	30.0	34.0
2	32.228903136371514	34.0	31.0	34.0	30.0	34.0
3	32.31450355224398	34.0	31.0	34.0	30.0	34.0
4	35.77196326459886	37.0	35.0	37.0	33.0	37.0
5	35.77612198925663	37.0	35.0	37.0	35.0	37.0
6	35.84751342921504	37.0	35.0	37.0	35.0	37.0
7	35.856177438918735	37.0	35.0	37.0	35.0	37.0
8	35.86900017328019	37.0	35.0	37.0	35.0	37.0
9	37.571651360249525	39.0	37.0	39.0	35.0	39.0
10	37.48258534049558	39.0	37.0	39.0	34.0	39.0
11	37.59209842315023	39.0	37.0	39.0	35.0	39.0
12	37.44481025818749	39.0	37.0	39.0	34.0	39.0
13	37.529544273089584	39.0	37.0	39.0	34.0	39.0
14	38.80159417778548	40.0	38.0	41.0	35.0	41.0
15	38.73557442384335	40.0	38.0	41.0	34.0	41.0
16	38.71183503725524	40.0	38.0	41.0	34.0	41.0
17	38.64564200311904	40.0	38.0	41.0	34.0	41.0
18	38.63957719632646	40.0	38.0	41.0	34.0	41.0
19	38.733668341708544	40.0	38.0	41.0	34.0	41.0
20	38.71893952521227	40.0	38.0	41.0	34.0	41.0
21	38.71599376191301	40.0	38.0	41.0	34.0	41.0
22	38.62814070351759	40.0	38.0	41.0	34.0	41.0
23	38.58741985791024	40.0	38.0	41.0	34.0	41.0
24	38.49246231155779	40.0	38.0	41.0	34.0	41.0
25	38.47565413273263	40.0	38.0	41.0	34.0	41.0
26	38.294403049731415	40.0	38.0	41.0	33.0	41.0
27	38.22786345520707	40.0	38.0	41.0	33.0	41.0
28	38.16496274475828	40.0	38.0	41.0	33.0	41.0
29	38.01386241552591	40.0	37.0	41.0	33.0	41.0
30	37.953907468376364	40.0	37.0	41.0	33.0	41.0
31	37.83070525038988	40.0	37.0	41.0	33.0	41.0
32	37.74874371859296	40.0	37.0	41.0	33.0	41.0
33	37.65707849592791	40.0	36.0	41.0	33.0	41.0
34	37.56316063073991	40.0	36.0	41.0	33.0	41.0
35	37.48223878010744	40.0	36.0	41.0	32.0	41.0
36	37.391959798994975	40.0	36.0	41.0	32.0	41.0
37	37.2117483971582	40.0	35.0	41.0	31.0	41.0
38	37.118177092358344	40.0	35.0	41.0	31.0	41.0
39	37.01732801940738	40.0	35.0	41.0	31.0	41.0
40	36.94472361809045	39.0	35.0	41.0	31.0	41.0
41	36.7399064286952	39.0	35.0	41.0	30.0	41.0
42	36.55051117657252	39.0	35.0	41.0	30.0	41.0
43	35.71963264598856	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	3.0
22	4.0
23	7.0
24	13.0
25	21.0
26	18.0
27	37.0
28	68.0
29	62.0
30	85.0
31	123.0
32	149.0
33	195.0
34	255.0
35	302.0
36	449.0
37	667.0
38	1047.0
39	2262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96118523652747	17.48397158204817	13.87974354531277	24.675099636111593
2	18.922197192860857	21.3481199098943	33.58170161150581	26.147981285739043
3	19.857910240859468	22.907641656558656	28.62588806099463	28.608560041587243
4	13.810431467683243	16.097730029457633	32.663316582914575	37.42852191994455
5	14.988736787385202	36.70074510483452	32.212788078322646	16.097730029457633
6	32.40339629180385	34.863975047652055	16.25368220412407	16.478946456420033
7	29.94281753595564	29.908161497140878	20.94957546352452	19.199445503378964
8	29.076416565586555	31.814243631952866	19.060821348119912	20.04851845434067
9	28.660544099809393	13.1866227690175	18.73158897937966	39.42124415179345
10	18.85288511523133	26.407901576849767	28.972448449142263	25.766764858776643
11	36.25021660024259	22.682377404262695	19.078149367527292	21.989256627967425
12	24.692427655518976	26.875758100849072	26.165309305146423	22.266504938485532
13	30.999826719805927	18.696932940564892	25.26425229596257	25.03898804366661
14	24.276555189741813	20.27378270663663	24.692427655518976	30.757234448102583
15	27.256974527811472	26.04401316929475	22.266504938485532	24.43250736440825
16	25.766764858776643	25.524172587073295	23.843354704557267	24.86570784959279
17	23.583434413446543	25.42020447062901	25.246924276555188	25.74943683936926
18	23.86068272396465	23.687402529890832	26.494541673886673	25.957373072257838
19	24.103274995667995	25.7147808005545	26.563853751516202	23.618090452261306
20	25.73210881996188	24.120603015075375	26.581181770923585	23.56610639403916
21	26.73713394559002	24.276555189741813	26.269277421590715	22.717033443077455
22	25.992029111072608	23.704730549298215	26.095997227516893	24.207243112112288
23	24.536475480852538	23.7220585687056	26.66782186796049	25.073644082481373
24	23.791370646335125	25.004332004851847	26.77178998440478	24.43250736440825
25	23.306186102928436	24.813723791370645	26.217293363368565	25.66279674233235
26	24.623115577889447	25.090972101888752	26.927742159071215	23.35817016115058
27	24.935019927222317	24.05129093744585	25.298908334777337	25.7147808005545
28	22.924969675966036	25.073644082481373	27.898111245884593	24.103274995667995
29	23.496794316409634	24.88303586900017	27.655518974181252	23.96465084040894
30	22.94229769537342	26.615837809738345	27.360942644255758	23.08092185063247
31	23.999306879223706	25.246924276555188	26.82377404262693	23.92999480159418
32	23.635418471668686	25.645468722924967	26.529197712701436	24.189915092704904
33	23.44481025818749	23.826026685149888	28.313983711661756	24.41517934500087
34	23.75671460752036	23.96465084040894	27.690175012996015	24.588459539074684
35	24.085946976260615	24.224571131519667	27.53422283832958	24.15525905389014
36	23.150233928261997	25.541500606480678	26.927742159071215	24.380523306186102
37	22.283832957892912	25.298908334777337	27.53422283832958	24.88303586900017
38	23.02893779241033	23.271530064113673	27.412926702477908	26.286605440998095
39	21.5387281233755	24.25922717033443	28.608560041587243	25.593484664702824
40	21.885288511523132	23.375498180557962	29.64824120603015	25.090972101888752
41	21.573384162190262	24.17258707329752	29.16305666262346	25.090972101888752
42	21.885288511523132	24.536475480852538	29.024432507364406	24.55380350025992
43	21.088199618783573	22.87298561774389	30.462658118177092	25.576156645295445
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	4.5
4	9.0
5	9.0
6	8.0
7	7.0
8	8.0
9	9.0
10	12.5
11	16.0
12	16.0
13	27.5
14	39.0
15	61.5
16	84.0
17	79.0
18	74.0
19	74.0
20	80.0
21	86.0
22	65.0
23	44.0
24	51.5
25	59.0
26	59.0
27	60.0
28	61.0
29	69.5
30	78.0
31	98.5
32	119.0
33	119.0
34	144.0
35	169.0
36	185.0
37	201.0
38	228.5
39	256.0
40	256.0
41	279.0
42	302.0
43	328.0
44	354.0
45	380.0
46	406.0
47	406.0
48	436.5
49	467.0
50	461.5
51	456.0
52	429.0
53	402.0
54	402.0
55	391.0
56	380.0
57	361.0
58	342.0
59	319.5
60	297.0
61	297.0
62	286.0
63	275.0
64	264.0
65	253.0
66	201.0
67	149.0
68	149.0
69	134.0
70	119.0
71	106.0
72	93.0
73	74.0
74	55.0
75	55.0
76	49.0
77	43.0
78	33.5
79	24.0
80	19.5
81	15.0
82	15.0
83	13.5
84	12.0
85	10.5
86	9.0
87	6.5
88	4.0
89	4.0
90	2.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5771.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.4645642003119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.95700174317257	84.0582221452088
2	4.10613984117761	7.347080228729856
3	1.1233778810768933	3.015075376884422
4	0.4261088514429595	1.5248657078495926
5	0.11621150493898895	0.5198405822214521
6	0.11621150493898895	0.6238086986657425
7	0.03873716831299632	0.2425922717033443
8	0.01936858415649816	0.1386241552590539
9	0.0	0.0
>10	0.09684292078249081	2.5298908334777335
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49	0.8490729509617051	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.6931207762952695	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34	0.5891526598509791	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	12	0.20793623288858082	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.19060821348119908	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	8	0.1386241552590539	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	7	0.12129613585167215	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	7	0.12129613585167215	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	6	0.10396811644429041	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG	6	0.10396811644429041	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	6	0.10396811644429041	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	6	0.10396811644429041	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.10396811644429041	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	6	0.10396811644429041	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.017328019407381736	0.0
23	0.0	0.0	0.0	0.03465603881476347	0.0
24	0.0	0.0	0.0	0.03465603881476347	0.0
25	0.0	0.0	0.0	0.03465603881476347	0.0
26	0.0	0.0	0.0	0.05198405822214521	0.0
27	0.0	0.0	0.0	0.08664009703690868	0.0
28	0.0	0.0	0.0	0.1906082134811991	0.0
29	0.0	0.0	0.0	0.36388840755501645	0.0
30	0.0	0.0	0.0	0.7104487957026512	0.0
31	0.0	0.0	0.0	1.4208975914053024	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE