FastQCFastQC Report
Fri 10 Feb 2017
ERR1631310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610057
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100970.6271206547345839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82500.5124042192295055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74830.4647661542417443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34270.21284960718782006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.019.1202321
CAGTCGG9250.018.610
GCAGTCG9350.018.5989329
TCGCTAC500.00703607918.49999815
AGTCGTA500.00703607918.49999830
CCGCTTA2550.018.13725525
TCGGTGA9750.017.83589713
CGCTTAT2500.017.7626
AGTCGGT9900.017.56565711
TTAACGG852.7242131E-517.41176435
ACCGTGT1107.8123776E-716.8181828
GGCAGTC10600.016.7547178
GTCGGTG10650.016.3286412
CGGTGAT10450.016.2870814
GTGATTC10700.015.73364516
CTTATAC30150.015.58540537
GTACTAT2500.015.541
TTGTATA1557.214112E-915.5161292
GGGCAGT11500.015.2826097
CGCCTTA7750.015.27741825
Produced by FastQC (version 0.11.5)