FastQCFastQC Report
Fri 10 Feb 2017
ERR1631309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16461
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA300.1822489520685256No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC250.15187412672377135No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC220.13364923151691877No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT210.12757426644796793TruSeq Adapter, Index 3 (95% over 21bp)
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC200.12149930137901707No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC200.12149930137901707No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG200.12149930137901707No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC200.12149930137901707No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG200.12149930137901707No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTCTCTTA170.1032744061721645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC656.389144E-519.92307737
CTCTTAT1701.9128493E-814.14705937
TCTTATA1000.001702734612.95000136
TCTCTTA2351.3007393E-610.23404336
GTCTCTT2707.674607E-68.90740835
TGTCTCT3452.179293E-68.04347837
CTGTCTC3901.2467008E-57.11538436
Produced by FastQC (version 0.11.5)