FastQCFastQC Report
Fri 10 Feb 2017
ERR1631308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042047
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40600.3896177427697599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36720.352383337795704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28250.27110101559718514No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18690.17935851261987223No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA16360.15699867664318407No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC14940.1433716521423698No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC14840.14241200252963637No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14600.14010884345907623No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC12630.12120374608822826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.10508163259430715No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC10900.10460180778794047No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA10690.10258654360120034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAC405.9396043E-527.7500021
GGTATCA26150.021.3652021
ACTGCGT450.00382557120.5555578
CGTAGTA450.00382557120.5555572
CGTTTGG555.1426515E-420.18181828
GTACCTA701.2190585E-418.51
TTACACT701.2190585E-418.54
TAATACT2350.017.3191474
CTACGGT752.0670332E-417.26666627
GTTTACA650.001579800517.0769211
TCTGTCG1851.8189894E-1217.08
TTGGTAA1201.0408985E-716.95833233
GTACTAT1201.0408985E-716.9583321
ATACCGC3400.016.86764727
GTTCAAA3300.016.8181821
TCTTATA11900.016.4789937
TACTGGT1751.3096724E-1015.8571437
TGGATAT700.002592275415.85714312
AGTAGTA700.002592275415.8571432
GTTTATA1059.341342E-615.8571421
Produced by FastQC (version 0.11.5)