##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631308.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1042047
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.056086721616204	34.0	33.0	34.0	31.0	34.0
2	33.19885667345139	34.0	33.0	34.0	31.0	34.0
3	33.2740788083455	34.0	34.0	34.0	31.0	34.0
4	36.49286644460375	37.0	37.0	37.0	35.0	37.0
5	36.498840263443014	37.0	37.0	37.0	35.0	37.0
6	36.564268214389564	37.0	37.0	37.0	35.0	37.0
7	36.56679785076873	37.0	37.0	37.0	35.0	37.0
8	36.56663566998417	37.0	37.0	37.0	35.0	37.0
9	38.41473849068228	39.0	39.0	39.0	37.0	39.0
10	38.37325955547111	39.0	39.0	39.0	37.0	39.0
11	38.44091965141688	39.0	39.0	39.0	37.0	39.0
12	38.39766056617408	39.0	39.0	39.0	37.0	39.0
13	38.422450234970206	39.0	39.0	39.0	37.0	39.0
14	39.96127717847659	41.0	40.0	41.0	38.0	41.0
15	39.95240809675571	41.0	40.0	41.0	38.0	41.0
16	39.90726234037428	41.0	40.0	41.0	38.0	41.0
17	39.893820528248725	41.0	40.0	41.0	38.0	41.0
18	39.890906072374854	41.0	40.0	41.0	38.0	41.0
19	39.91827336003079	41.0	40.0	41.0	38.0	41.0
20	39.908115468880005	41.0	40.0	41.0	38.0	41.0
21	39.87808419389912	41.0	40.0	41.0	38.0	41.0
22	39.8232018325469	41.0	40.0	41.0	38.0	41.0
23	39.75209947344026	41.0	40.0	41.0	38.0	41.0
24	39.73079333273835	41.0	40.0	41.0	37.0	41.0
25	39.70161806521203	41.0	40.0	41.0	37.0	41.0
26	39.61897304056343	41.0	40.0	41.0	37.0	41.0
27	39.549170047032426	41.0	40.0	41.0	37.0	41.0
28	39.46221235702421	41.0	39.0	41.0	36.0	41.0
29	39.39557332826638	41.0	39.0	41.0	36.0	41.0
30	39.31101380264038	41.0	39.0	41.0	36.0	41.0
31	39.23889805354269	41.0	39.0	41.0	35.0	41.0
32	39.14986752037096	41.0	39.0	41.0	35.0	41.0
33	39.04099143320791	41.0	39.0	41.0	35.0	41.0
34	38.97360195845293	41.0	39.0	41.0	35.0	41.0
35	38.86108400100955	41.0	39.0	41.0	35.0	41.0
36	38.781291055009994	40.0	38.0	41.0	35.0	41.0
37	38.69568647095573	40.0	38.0	41.0	35.0	41.0
38	38.59610075169354	40.0	38.0	41.0	35.0	41.0
39	38.48099845784307	40.0	38.0	41.0	35.0	41.0
40	38.36687404694798	40.0	38.0	41.0	35.0	41.0
41	38.256840622351966	40.0	37.0	41.0	35.0	41.0
42	38.10628407355906	40.0	37.0	41.0	34.0	41.0
43	37.277338738079955	40.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	2.0
13	1.0
14	0.0
15	5.0
16	7.0
17	10.0
18	10.0
19	32.0
20	61.0
21	130.0
22	267.0
23	363.0
24	628.0
25	968.0
26	1455.0
27	1970.0
28	2938.0
29	4112.0
30	5462.0
31	6800.0
32	8687.0
33	11679.0
34	18025.0
35	27679.0
36	45738.0
37	94327.0
38	207745.0
39	602941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15299885705731	17.76330626161776	12.036117372824833	26.047577508500098
2	20.854049769348215	19.653815998702555	32.34566195190812	27.146472280041113
3	20.78034867909029	21.14261640789715	29.132659083515428	28.944375829497133
4	14.356838031298015	15.568011807528833	33.869009747161115	36.20614041401203
5	15.935653574167	35.19630112653268	32.477613773658966	16.390431525641358
6	34.578094846009826	33.29187647006325	16.07643417235499	16.053594511571934
7	30.428282025666785	29.90412140719181	19.462749760807334	20.20484680633407
8	30.28682967274989	29.28965776015861	19.01468935662211	21.408823210469393
9	28.062265905472593	13.003828042305193	19.055762360047098	39.87814369217511
10	19.573973150923134	24.271745900136942	29.97235249465715	26.181928454282772
11	37.74407488337858	20.933988582088908	18.81661767655394	22.505318857978576
12	23.910725715826636	25.418239292469536	25.824938798345947	24.84609619335788
13	32.774241468954855	17.65476989041761	23.101549162369835	26.4694394782577
14	24.315889782322678	19.99765845494493	22.97813822217232	32.70831354056007
15	29.246665457508154	24.723261042928005	20.49043853108353	25.539634968480307
16	27.318921315449302	23.941434503434106	22.44649233671802	26.293151844398572
17	25.23590586605019	24.619522919791525	23.350674201835425	26.79389701232286
18	25.801811242679072	21.87972327543767	25.453266503334305	26.865198978548953
19	26.048057333306463	24.102079848605676	24.510698653707557	25.3391641643803
20	27.264701112329863	21.923963122584684	24.854541109949935	25.956794655135518
21	28.888908081881144	22.60656189212195	23.437426526826524	25.067103499170386
22	28.543242291374572	22.095932333186507	23.293191190032694	26.067634185406224
23	26.743707337576904	22.27385137138728	23.856601477668473	27.125839813367342
24	26.006408540113835	23.22342466318698	24.084998085499024	26.68516871120017
25	26.380383994196038	22.495626396889968	23.81744777346895	27.306541835445042
26	26.523083891609495	23.041858956457816	23.694804552961624	26.740252598971065
27	26.82844439838126	22.162532016310205	23.85880867177776	27.150214913530775
28	25.605083072068723	22.69072316315867	24.814619686060226	26.889574078712382
29	24.974017486735242	23.253461696065532	25.468620897138038	26.303899920061184
30	24.872678487630594	24.167815847077915	25.590496397955175	25.369009267336313
31	26.246896733064823	24.259462385093954	23.43646687721379	26.05717400462743
32	25.03265207807325	23.440209510703454	24.85060654653773	26.676531864685565
33	24.735832452854815	22.588712409325108	25.51506793839433	27.160387199425745
34	26.041531715939875	22.539482384191885	25.066911569247836	26.352074330620407
35	25.63473624510219	23.132545844861124	25.44808439542554	25.784633514611144
36	24.39698017459865	24.480181796022638	24.8420656649844	26.280772364394313
37	25.559787610347712	23.409404758134713	25.17458425579652	25.856223375721054
38	24.723452972850552	22.758762320701464	26.0907617410731	26.42702296537488
39	25.03217225326689	22.674505084703473	26.12031894914529	26.173003712884352
40	24.16224987932406	22.510309035964788	26.818368077447563	26.509073007263588
41	23.15260252176725	22.82296287979333	27.560081263129206	26.464353335310207
42	23.838847959832908	22.857030441045364	27.555570909949363	25.74855068917237
43	22.72008844130831	22.190361855079473	27.778209620103507	27.31134008350871
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	46.0
2	72.0
3	246.5
4	421.0
5	421.0
6	542.0
7	663.0
8	624.0
9	585.0
10	848.0
11	1111.0
12	1111.0
13	2025.5
14	2940.0
15	4834.0
16	6728.0
17	6148.0
18	5568.0
19	5568.0
20	6725.5
21	7883.0
22	6215.0
23	4547.0
24	5288.0
25	6029.0
26	6029.0
27	7251.0
28	8473.0
29	10726.5
30	12980.0
31	15725.0
32	18470.0
33	18470.0
34	21940.5
35	25411.0
36	29788.0
37	34165.0
38	36899.5
39	39634.0
40	39634.0
41	44040.0
42	48446.0
43	51315.0
44	54184.0
45	57906.5
46	61629.0
47	61629.0
48	68923.0
49	76217.0
50	78150.0
51	80083.0
52	79285.0
53	78487.0
54	78487.0
55	77413.5
56	76340.0
57	74384.5
58	72429.0
59	70884.0
60	69339.0
61	69339.0
62	66451.0
63	63563.0
64	57666.5
65	51770.0
66	46770.5
67	41771.0
68	41771.0
69	36219.5
70	30668.0
71	26692.0
72	22716.0
73	17805.0
74	12894.0
75	12894.0
76	10380.5
77	7867.0
78	6775.5
79	5684.0
80	5020.0
81	4356.0
82	4356.0
83	3665.5
84	2975.0
85	2555.5
86	2136.0
87	1862.5
88	1589.0
89	1589.0
90	1223.0
91	857.0
92	559.0
93	261.0
94	166.5
95	72.0
96	72.0
97	42.0
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1042047.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.07333591226146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22541525521595	53.29916263194651
2	5.971182448759964	7.0547533317799465
3	1.1385580148693737	2.0177526020392826
4	0.5395965432180931	1.275030714184701
5	0.32623973583290244	0.9636034751392244
6	0.2390889312390953	0.8474268448794395
7	0.17479225091017483	0.7227892947013932
8	0.1355679549519352	0.6406761073451198
9	0.11302687486083093	0.6009187091190306
>10	0.9024051571312993	10.908308333977343
>50	0.12604983070462755	5.206327805885589
>100	0.09893453707909974	11.36850182859189
>500	0.007183365535263202	2.874939849535993
>1k	0.001959099691435419	2.219808470874537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4060	0.3896177427697599	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3672	0.352383337795704	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2825	0.27110101559718514	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1869	0.17935851261987223	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1636	0.15699867664318407	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1494	0.1433716521423698	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1484	0.14241200252963637	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1460	0.14010884345907623	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1263	0.12120374608822826	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1095	0.10508163259430715	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1090	0.10460180778794047	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1069	0.10258654360120034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.596496127333988E-5	0.0
10	0.0	0.0	0.0	9.596496127333988E-5	0.0
11	0.0	0.0	0.0	9.596496127333988E-5	0.0
12	0.0	0.0	0.0	1.9192992254667975E-4	0.0
13	0.0	0.0	0.0	2.8789488382001963E-4	0.0
14	0.0	0.0	0.0	6.717547289133792E-4	0.0
15	0.0	0.0	0.0	9.596496127333988E-4	0.0
16	0.0	0.0	0.0	0.002399124031833497	0.0
17	0.0	0.0	0.0	0.0051821079087603535	0.0
18	0.0	0.0	0.0	0.005853862637673733	0.0
19	0.0	0.0	0.0	0.007197372095500491	0.0
20	0.0	0.0	0.0	0.008828776437147268	0.0
21	0.0	0.0	0.0	0.011323865430254106	0.0
22	0.0	0.0	0.0	0.018713167448301277	0.0
23	0.0	0.0	0.0	0.02994106791728204	0.0
24	0.0	0.0	0.0	0.04567932156610978	0.0
25	0.0	0.0	0.0	0.050573534591050115	0.0
26	0.0	0.0	0.0	0.0596902059120174	0.0
27	0.0	0.0	0.0	0.07705986390249192	0.0
28	0.0	0.0	0.0	0.13367919105376244	0.0
29	0.0	0.0	0.0	0.21764853216793484	0.0
30	0.0	0.0	0.0	0.3461456153129369	0.0
31	0.0	0.0	0.0	0.6738659580613926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAC	40	5.9396043E-5	27.750002	1
GGTATCA	2615	0.0	21.365202	1
ACTGCGT	45	0.003825571	20.555557	8
CGTAGTA	45	0.003825571	20.555557	2
CGTTTGG	55	5.1426515E-4	20.181818	28
GTACCTA	70	1.2190585E-4	18.5	1
TTACACT	70	1.2190585E-4	18.5	4
TAATACT	235	0.0	17.319147	4
CTACGGT	75	2.0670332E-4	17.266666	27
GTTTACA	65	0.0015798005	17.076921	1
TCTGTCG	185	1.8189894E-12	17.0	8
TTGGTAA	120	1.0408985E-7	16.958332	33
GTACTAT	120	1.0408985E-7	16.958332	1
ATACCGC	340	0.0	16.867647	27
GTTCAAA	330	0.0	16.818182	1
TCTTATA	1190	0.0	16.47899	37
TACTGGT	175	1.3096724E-10	15.857143	7
TGGATAT	70	0.0025922754	15.857143	12
AGTAGTA	70	0.0025922754	15.857143	2
GTTTATA	105	9.341342E-6	15.857142	1
>>END_MODULE