FastQCFastQC Report
Fri 10 Feb 2017
ERR1631307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640274
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31780.19374811769253186No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC28000.1707031873943012No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC22650.1380866855171758No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22390.13650158449137156No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC21530.13125855802140374No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT21120.12875897563455863No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC20660.12595456612736652No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG19810.1207725050814681No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC19220.11717554506137388No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17770.10833555857131187No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA17130.10443377143087071No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG16990.10358025549389918No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC16920.10315349752541343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.10223901616437253No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC16490.10053198429042952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA250.005497019729.615
GGTATCA10600.026.0047171
TGTGCGA500.007036132318.510
TGCGCTA500.007036132318.510
CGCACTA500.007036132318.512
CTTATAC16550.016.6555937
GTATCAA16650.016.4444452
GACGGAC5200.016.0096157
GCCGTTA1557.214112E-915.516129515
GTATATA1201.936629E-615.4166671
GTATAAA1455.349466E-815.3103451
CATATAA1954.1836756E-1115.1794862
TAGTACC1101.4525416E-515.1363644
AAGACGG5500.014.85
CGCCTTA1252.9604253E-614.79999925
TCTATGG2900.014.6724142
AGACGGA5850.014.5470096
GTTTTCG7000.014.53571428
GTGTTAG1152.2108705E-514.4782611
ATACTGG2301.8189894E-1214.4782616
Produced by FastQC (version 0.11.5)