FastQCFastQC Report
Fri 10 Feb 2017
ERR1631306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14873
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA690.4639279230820951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT640.4303099576413635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT520.3496268405836079No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC490.32945606131916894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT460.30928528205473005No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC410.2756673166139985No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC390.26222013043770587No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC380.2554965373495596No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC350.2353257580851207No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT330.22187857190882807No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG290.19498419955624285No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC290.19498419955624285No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC290.19498419955624285No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT290.19498419955624285No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC280.18826060646809653No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC280.18826060646809653No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC280.18826060646809653No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG260.17481342029180394No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG260.17481342029180394No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG240.16136623411551135No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT230.15464264102736502No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC230.15464264102736502No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA230.15464264102736502No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC220.14791904793921873No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA220.14791904793921873No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG220.14791904793921873No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG220.14791904793921873No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC210.1411954548510724No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT210.1411954548510724No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC200.1344718617629261No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC200.1344718617629261No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC200.1344718617629261No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG180.12102467558663349No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT170.1143010824984872No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA170.1143010824984872No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG170.1143010824984872No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC160.10757748941034087No Hit
CGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATT150.10085389632219458No Hit
CGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCA150.10085389632219458No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGG150.10085389632219458No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC150.10085389632219458No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG150.10085389632219458No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC150.10085389632219458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA555.592956E-726.909091
GTATCAA704.5665674E-621.1428572
ATCAACG1103.1504896E-818.52
AACGCAG1155.2439646E-817.695655
TCAACGC1155.2439646E-817.695653
CAACGCA1155.2439646E-817.695654
GAGTACA1054.061476E-717.6190511
ACGCAGA1208.5325155E-816.9583326
GCAGAGT1208.5325155E-816.9583328
CGCAGAG1208.5325155E-816.9583327
AGTACAT1151.0534095E-616.08695612
CATGGGA700.002434612715.85714216
CAGAGTA1302.1254891E-715.6538469
TATCAAC1302.1254891E-715.6538461
AGAGTAC1353.2641037E-715.07407510
ACATGGG1408.140365E-613.21428515
TACATGG1408.140365E-613.21428514
GTACATG1451.1693628E-512.7586213
CCTGTCT1204.6155285E-412.33333337
Produced by FastQC (version 0.11.5)