##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631306.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14873
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.811470449808375	34.0	31.0	34.0	31.0	34.0
2	32.95710347609763	34.0	33.0	34.0	31.0	34.0
3	33.013043770591004	34.0	33.0	34.0	31.0	34.0
4	36.320984334028104	37.0	37.0	37.0	35.0	37.0
5	36.31190748335911	37.0	37.0	37.0	35.0	37.0
6	36.372823236737716	37.0	37.0	37.0	35.0	37.0
7	36.38613595105224	37.0	37.0	37.0	35.0	37.0
8	36.38835473677133	37.0	37.0	37.0	35.0	37.0
9	38.17467894843004	39.0	39.0	39.0	37.0	39.0
10	38.14428830767162	39.0	39.0	39.0	37.0	39.0
11	38.18765548309016	39.0	39.0	39.0	37.0	39.0
12	38.132118604182075	39.0	39.0	39.0	37.0	39.0
13	38.176561554494725	39.0	39.0	39.0	37.0	39.0
14	39.63289181738721	41.0	40.0	41.0	37.0	41.0
15	39.624016674510855	41.0	40.0	41.0	37.0	41.0
16	39.558932293417605	41.0	39.0	41.0	37.0	41.0
17	39.548376252269215	41.0	39.0	41.0	37.0	41.0
18	39.5328447522356	41.0	39.0	41.0	37.0	41.0
19	39.5571169232838	41.0	40.0	41.0	37.0	41.0
20	39.56847979560277	41.0	40.0	41.0	37.0	41.0
21	39.554830901633835	41.0	40.0	41.0	37.0	41.0
22	39.490217172056745	41.0	39.0	41.0	37.0	41.0
23	39.36946144019364	41.0	39.0	41.0	36.0	41.0
24	39.36858737309218	41.0	39.0	41.0	36.0	41.0
25	39.355409130639416	41.0	39.0	41.0	36.0	41.0
26	39.24406642909971	41.0	39.0	41.0	36.0	41.0
27	39.17319975795065	41.0	39.0	41.0	36.0	41.0
28	39.04686344382438	41.0	39.0	41.0	35.0	41.0
29	38.98211524238553	41.0	39.0	41.0	35.0	41.0
30	38.942311571303705	40.0	39.0	41.0	35.0	41.0
31	38.90035635043367	40.0	38.0	41.0	35.0	41.0
32	38.777785248436764	40.0	38.0	41.0	35.0	41.0
33	38.6772002958381	40.0	38.0	41.0	35.0	41.0
34	38.58945740603779	40.0	38.0	41.0	35.0	41.0
35	38.45949035164392	40.0	38.0	41.0	34.0	41.0
36	38.44153835809857	40.0	38.0	41.0	35.0	41.0
37	38.33322127344853	40.0	38.0	41.0	34.0	41.0
38	38.24050292476299	40.0	37.0	41.0	34.0	41.0
39	38.15276003496268	40.0	37.0	41.0	34.0	41.0
40	37.951522893834465	40.0	37.0	41.0	34.0	41.0
41	37.85248436764607	40.0	37.0	41.0	34.0	41.0
42	37.69777449068782	40.0	36.0	41.0	33.0	41.0
43	36.886640220533856	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	4.0
22	2.0
23	6.0
24	15.0
25	21.0
26	41.0
27	37.0
28	55.0
29	90.0
30	96.0
31	142.0
32	173.0
33	268.0
34	337.0
35	519.0
36	861.0
37	1446.0
38	3289.0
39	7466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47475290795401	17.26618705035971	12.909298729240906	26.34976131244537
2	21.448261951186716	19.323606535332484	31.358838163114367	27.869293350366437
3	21.36757883412896	20.896927317958717	28.555099845357358	29.180394002554966
4	13.978350030256168	15.753378605526795	33.301956565588654	36.96631479862839
5	16.217306528608887	36.07880051099308	31.325220197673637	16.3786727627244
6	34.781147044980834	33.449875613527865	15.820614536408256	15.948362805083036
7	30.686478854299736	29.630874739460765	18.610905667988973	21.071740738250522
8	31.45296846634842	27.916358501983463	18.22093726887649	22.409735762791634
9	28.04410677065824	12.842062798359443	18.92691454313185	40.18691588785047
10	21.024675586633496	23.956162173065287	28.60888859006253	26.410273650238686
11	38.70772540845828	21.407920392657836	17.48806562226854	22.39628857661534
12	24.890741612317623	24.816782088348013	24.648692261144355	25.64378403819001
13	33.053183621327236	17.62253748403147	23.303973643515093	26.020305251126203
14	25.099172998050157	19.814428830767163	21.488603509715592	33.59779466146709
15	30.000672359308815	24.984871915551672	19.51186714180058	25.502588583338937
16	28.299603308007796	23.942714986888994	21.555839440597055	26.201842264506155
17	27.73482148860351	24.21838230350299	21.609628185302228	26.437168022591273
18	26.564916291266055	20.950716062663886	24.305789013648894	28.178578632421164
19	26.75317689773415	24.245276675855575	22.39628857661534	26.605257849794928
20	28.420627983594432	20.01613662341155	23.626706111746117	27.9365292812479
21	30.42425872386203	22.335776238822028	22.342499831910175	24.89746520540577
22	31.251260673704028	20.2985275331137	22.134068446177636	26.31614334700464
23	27.37174746184361	21.4751563235393	22.907281651314463	28.24581456330263
24	25.596718886572983	23.035029919989242	23.371209574396556	27.997041619041212
25	26.78679486317488	21.12552948295569	23.25690849189807	28.83076716197136
26	27.438983392725074	22.698850265581928	22.934176023667046	26.92799031802595
27	28.736636858737306	21.065017145162372	23.12916022322329	27.069185772877024
28	25.64378403819001	22.26854030794056	23.922544207624554	28.165131446244875
29	24.527667585557722	21.91891346735696	25.616889665837423	27.9365292812479
30	23.411551132925435	24.76299334364284	25.556377328044107	26.269078195387614
31	26.759900490822297	23.962885766153434	21.905466281180665	27.37174746184361
32	26.24218382303503	22.174410004706516	24.44026087541182	27.143145296846633
33	24.843676460700596	22.234922342499832	24.46043165467626	28.46096954212331
34	25.932898540980297	22.329052645733878	24.843676460700596	26.894372352585222
35	24.689033819673234	22.651785113964902	25.85893901701069	26.800242049351176
36	24.124252000268946	24.258723862031868	24.668863040408795	26.94816109729039
37	26.605257849794928	22.49041887984939	25.179856115107913	25.724467155247766
38	24.984871915551672	20.681772339138035	25.973240099509177	28.360115645801116
39	25.2941571976064	21.360855241040813	26.511127546560882	26.833860014791906
40	23.324144422779533	20.540576884286963	28.21892019095004	27.916358501983463
41	22.806427754992267	21.414643985745982	29.193841188731255	26.585087070530495
42	23.73428360115646	20.634707187521013	29.846029718281446	25.78497949304108
43	21.46170913736301	20.56747125663955	29.1131580716735	28.85766153432394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	4.5
4	7.0
5	7.0
6	6.5
7	6.0
8	7.5
9	9.0
10	14.5
11	20.0
12	20.0
13	30.0
14	40.0
15	69.5
16	99.0
17	92.5
18	86.0
19	86.0
20	101.5
21	117.0
22	80.5
23	44.0
24	49.5
25	55.0
26	55.0
27	66.0
28	77.0
29	101.5
30	126.0
31	144.0
32	162.0
33	162.0
34	197.5
35	233.0
36	268.0
37	303.0
38	371.0
39	439.0
40	439.0
41	520.0
42	601.0
43	635.0
44	669.0
45	762.0
46	855.0
47	855.0
48	1030.0
49	1205.0
50	1186.0
51	1167.0
52	1274.0
53	1381.0
54	1381.0
55	1266.0
56	1151.0
57	1098.0
58	1045.0
59	1067.0
60	1089.0
61	1089.0
62	1089.5
63	1090.0
64	1017.0
65	944.0
66	821.0
67	698.0
68	698.0
69	593.5
70	489.0
71	394.5
72	300.0
73	215.5
74	131.0
75	131.0
76	105.0
77	79.0
78	65.5
79	52.0
80	41.5
81	31.0
82	31.0
83	28.0
84	25.0
85	24.5
86	24.0
87	19.0
88	14.0
89	14.0
90	10.0
91	6.0
92	3.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	14873.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.02857527062461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.24106102697374	67.7065823976333
2	5.009409445290797	7.516977072547569
3	1.81019804641993	4.074497411416661
4	0.7886011291334349	2.3667047670274997
5	0.41222331750156826	1.5464264102736502
6	0.3136481763598889	1.411954548510724
7	0.25091854108791106	1.3178242452766757
8	0.15234339994623175	0.9144086599878976
9	0.12545927054395553	0.8471727291064345
>10	0.8692535173402636	11.053587036912527
>50	0.026884129402276188	1.2438647213070664
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	69	0.4639279230820951	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	64	0.4303099576413635	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	52	0.3496268405836079	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	49	0.32945606131916894	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.30928528205473005	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	41	0.2756673166139985	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	39	0.26222013043770587	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	38	0.2554965373495596	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	35	0.2353257580851207	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	33	0.22187857190882807	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	29	0.19498419955624285	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	29	0.19498419955624285	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	29	0.19498419955624285	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	29	0.19498419955624285	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	28	0.18826060646809653	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	28	0.18826060646809653	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC	28	0.18826060646809653	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	26	0.17481342029180394	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	26	0.17481342029180394	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	24	0.16136623411551135	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	23	0.15464264102736502	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC	23	0.15464264102736502	No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA	23	0.15464264102736502	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	22	0.14791904793921873	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	22	0.14791904793921873	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	22	0.14791904793921873	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	22	0.14791904793921873	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	21	0.1411954548510724	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	21	0.1411954548510724	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	20	0.1344718617629261	No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	20	0.1344718617629261	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC	20	0.1344718617629261	No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG	18	0.12102467558663349	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	17	0.1143010824984872	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	17	0.1143010824984872	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	17	0.1143010824984872	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	16	0.10757748941034087	No Hit
CGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATT	15	0.10085389632219458	No Hit
CGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCA	15	0.10085389632219458	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGG	15	0.10085389632219458	No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC	15	0.10085389632219458	No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG	15	0.10085389632219458	No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC	15	0.10085389632219458	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.006723593088146305	0.0
16	0.0	0.0	0.0	0.006723593088146305	0.0
17	0.0	0.0	0.0	0.006723593088146305	0.0
18	0.0	0.0	0.0	0.006723593088146305	0.0
19	0.0	0.0	0.0	0.006723593088146305	0.0
20	0.0	0.0	0.0	0.01344718617629261	0.0
21	0.0	0.0	0.0	0.01344718617629261	0.0
22	0.0	0.0	0.0	0.020170779264438915	0.0
23	0.0	0.0	0.0	0.02689437235258522	0.0
24	0.0	0.0	0.0	0.04706515161702414	0.0
25	0.0	0.0	0.0	0.05378874470517044	0.0
26	0.0	0.0	0.0	0.06723593088146305	0.0
27	0.0	0.0	0.0	0.08068311705775566	0.0
28	0.0	0.0	0.0	0.16808982720365764	0.0
29	0.0	0.0	0.0	0.329456061319169	0.0
30	0.0	0.0	0.0	0.551334633227997	0.0
31	0.0	0.0	0.0	1.1295636388085792	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	55	5.592956E-7	26.90909	1
GTATCAA	70	4.5665674E-6	21.142857	2
ATCAACG	110	3.1504896E-8	18.5	2
AACGCAG	115	5.2439646E-8	17.69565	5
TCAACGC	115	5.2439646E-8	17.69565	3
CAACGCA	115	5.2439646E-8	17.69565	4
GAGTACA	105	4.061476E-7	17.61905	11
ACGCAGA	120	8.5325155E-8	16.958332	6
GCAGAGT	120	8.5325155E-8	16.958332	8
CGCAGAG	120	8.5325155E-8	16.958332	7
AGTACAT	115	1.0534095E-6	16.086956	12
CATGGGA	70	0.0024346127	15.857142	16
CAGAGTA	130	2.1254891E-7	15.653846	9
TATCAAC	130	2.1254891E-7	15.653846	1
AGAGTAC	135	3.2641037E-7	15.074075	10
ACATGGG	140	8.140365E-6	13.214285	15
TACATGG	140	8.140365E-6	13.214285	14
GTACATG	145	1.1693628E-5	12.75862	13
CCTGTCT	120	4.6155285E-4	12.333333	37
>>END_MODULE