FastQCFastQC Report
Fri 10 Feb 2017
ERR1631305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757623
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12960.1710613326152981No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9910.130803843072346No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9270.12235636985677573No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9250.12209238631878917No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT8250.1088932094194606No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8030.10598939050160833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.024.6666681
TAAACTA652.680843E-622.769235
CTTATAC10550.020.3412337
ATACACA2100.020.26190637
CTATGCT555.1408686E-420.1818184
CCCAATA656.898163E-519.9230772
CGAACGA1950.018.97435816
TAATATG500.00703272218.54
CTAGAAC609.2328567E-418.53
GCTATTC500.00703272218.53
CTAAACT801.6154852E-518.54
GTACTAT701.2185014E-418.51
TCGGTTG801.6154852E-518.532
ATTTCGG801.6154852E-518.529
TTTAGAG1054.7919275E-717.619053
TAACGAA2000.017.57513
TTTCGGT852.7211318E-517.41176430
GTCCTAA1502.5102054E-1017.2666661
GTGTACA752.0660947E-417.2666661
ATAACGA2150.017.20930312
Produced by FastQC (version 0.11.5)