##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631305.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757623
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00649267511678	34.0	33.0	34.0	31.0	34.0
2	33.15836768419121	34.0	33.0	34.0	31.0	34.0
3	33.249164822081696	34.0	34.0	34.0	31.0	34.0
4	36.493548902290456	37.0	37.0	37.0	35.0	37.0
5	36.47528124146178	37.0	37.0	37.0	35.0	37.0
6	36.52596476083752	37.0	37.0	37.0	35.0	37.0
7	36.52896625366442	37.0	37.0	37.0	35.0	37.0
8	36.52261876949353	37.0	37.0	37.0	35.0	37.0
9	38.369283931454035	39.0	39.0	39.0	37.0	39.0
10	38.31886571553398	39.0	39.0	39.0	37.0	39.0
11	38.38321698258897	39.0	39.0	39.0	37.0	39.0
12	38.3238853625088	39.0	39.0	39.0	37.0	39.0
13	38.36224217057824	39.0	39.0	39.0	37.0	39.0
14	39.89639966051717	41.0	40.0	41.0	38.0	41.0
15	39.894409224640754	41.0	40.0	41.0	38.0	41.0
16	39.85805473170693	41.0	40.0	41.0	38.0	41.0
17	39.84424311299947	41.0	40.0	41.0	38.0	41.0
18	39.817636212205805	41.0	40.0	41.0	38.0	41.0
19	39.82832754549426	41.0	40.0	41.0	38.0	41.0
20	39.80781470467502	41.0	40.0	41.0	38.0	41.0
21	39.77333185502552	41.0	40.0	41.0	38.0	41.0
22	39.71710732118745	41.0	40.0	41.0	38.0	41.0
23	39.652794331745476	41.0	40.0	41.0	37.0	41.0
24	39.62334696808307	41.0	40.0	41.0	37.0	41.0
25	39.60855201069661	41.0	40.0	41.0	37.0	41.0
26	39.51426105068088	41.0	40.0	41.0	37.0	41.0
27	39.45643413676723	41.0	39.0	41.0	37.0	41.0
28	39.37247549242829	41.0	39.0	41.0	36.0	41.0
29	39.315272899581984	41.0	39.0	41.0	36.0	41.0
30	39.245426815183805	41.0	39.0	41.0	36.0	41.0
31	39.2104080789522	41.0	39.0	41.0	35.0	41.0
32	39.13256593318841	41.0	39.0	41.0	35.0	41.0
33	39.05132103961997	41.0	39.0	41.0	35.0	41.0
34	38.99704470429224	40.0	39.0	41.0	35.0	41.0
35	38.8923831509867	40.0	39.0	41.0	35.0	41.0
36	38.8354683001968	40.0	38.0	41.0	35.0	41.0
37	38.77196574021644	40.0	38.0	41.0	35.0	41.0
38	38.70153625219931	40.0	38.0	41.0	35.0	41.0
39	38.624016166351865	40.0	38.0	41.0	35.0	41.0
40	38.52729787770435	40.0	38.0	41.0	35.0	41.0
41	38.44247336736081	40.0	37.0	41.0	35.0	41.0
42	38.312654182885154	40.0	37.0	41.0	34.0	41.0
43	37.46783426585518	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	0.0
15	2.0
16	4.0
17	2.0
18	12.0
19	20.0
20	53.0
21	100.0
22	157.0
23	288.0
24	460.0
25	679.0
26	969.0
27	1408.0
28	1906.0
29	2711.0
30	3678.0
31	4918.0
32	6479.0
33	8822.0
34	13786.0
35	20841.0
36	35089.0
37	66689.0
38	161229.0
39	427318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6169440473692	17.096365870624307	11.64378589351168	27.642904188494803
2	20.29056668026182	18.86809138582118	32.09049883649256	28.750843097424443
3	20.538975189507184	20.31353324806665	28.023304466733457	31.12418709569271
4	15.005616249770664	14.755491847528388	32.643808332112414	37.59508357058854
5	17.25198416626739	33.704230204204464	31.855421495915515	17.188364133612627
6	36.83058724457943	32.69356922902288	15.033466513028248	15.442377013369446
7	32.22816625155255	28.119658458098552	19.391834725186538	20.26034056516236
8	29.77259138120147	30.323789008517426	18.55065118139233	21.352968428888776
9	27.999149973007682	13.393204799748688	18.048554492141868	40.55909073510176
10	19.269742338867747	23.796532048261472	29.7168908546863	27.216834758184476
11	39.86019431828231	19.721154188824784	19.21443778765956	21.20421370523334
12	24.584390917382393	23.356867465744834	25.94826186639001	26.110479750482764
13	32.47512285133899	16.742759921491295	22.92274653752592	27.859370689643793
14	24.562084308422527	18.722768448159574	21.835398344559234	34.879748898858665
15	28.133913569149826	24.9715227758397	19.515247029195258	27.379316625815214
16	28.188030194437076	23.384717729002418	21.331321778773876	27.09593029778663
17	27.027558561448107	23.48516346520631	22.186628441850367	27.300649531495214
18	27.35542611562743	21.653777670424475	23.62982644402295	27.36096976992515
19	27.38380434596099	22.943601237026858	22.662986736147133	27.009607680865024
20	27.679465908505946	21.79144508548447	22.48004614432244	28.049042861687145
21	29.429280790049933	22.48492983977519	21.973329743157215	26.11245962701766
22	29.100357301718667	21.883311356703796	21.876579776485137	27.1397515650924
23	27.435149144099373	21.70380255087293	22.638964234190357	28.222084070837344
24	26.825743146657377	22.932513928431423	22.49245337060781	27.74928955430339
25	27.266067688018975	21.741684188574002	22.49601714837063	28.496230975036397
26	27.268443539860854	23.0135568745933	22.649919551016797	27.068080034529046
27	28.00192180015654	21.786033422955743	22.506708481659086	27.705336295228626
28	26.924868965171335	22.308192861093183	23.178810569372892	27.588127604362594
29	26.228744375500746	22.668662382213846	23.73594782629355	27.366645415991858
30	25.69219783454304	23.528852740743087	23.75033492911382	27.028614495600056
31	27.290486165282736	22.96617182952471	22.152442573681107	27.590899431511453
32	26.2080216677688	22.467110950961096	22.766600274806866	28.55826710646324
33	25.74288267383646	21.93769196552903	23.895789858544422	28.42363550209009
34	27.177765194562465	21.650213892661654	23.86002008914724	27.31200082362864
35	26.89055110523308	21.65087385150662	24.507967683135277	26.950607360125023
36	25.34756732570157	22.602402514179214	24.362644745473673	27.687385414645544
37	26.144401635114033	21.15075703879106	24.912258471561714	27.792582854533194
38	24.972446718222653	20.633877271413354	26.19601041679041	28.197665593573586
39	25.27774367990412	20.198436425504507	26.549880349461407	27.973939545129966
40	24.601417855582525	20.623317929893894	27.35951786046622	27.41574635405736
41	23.503114345789395	20.69036974854248	28.665180439347804	27.14133546632032
42	23.952678310980527	20.83226090021026	28.712301500878407	26.502759287930804
43	22.39570340393573	19.91980180115968	28.989880191071283	28.69461460383331
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	11.5
2	11.0
3	35.5
4	60.0
5	60.0
6	95.0
7	130.0
8	134.5
9	139.0
10	216.5
11	294.0
12	294.0
13	485.0
14	676.0
15	1075.5
16	1475.0
17	1431.0
18	1387.0
19	1387.0
20	1580.0
21	1773.0
22	1684.5
23	1596.0
24	1998.5
25	2401.0
26	2401.0
27	3014.5
28	3628.0
29	4885.0
30	6142.0
31	7572.5
32	9003.0
33	9003.0
34	11366.0
35	13729.0
36	17114.0
37	20499.0
38	24255.0
39	28011.0
40	28011.0
41	30925.5
42	33840.0
43	36726.5
44	39613.0
45	42868.0
46	46123.0
47	46123.0
48	49852.5
49	53582.0
50	55455.0
51	57328.0
52	59534.5
53	61741.0
54	61741.0
55	60724.5
56	59708.0
57	58903.5
58	58099.0
59	57438.0
60	56777.0
61	56777.0
62	53436.5
63	50096.0
64	46656.5
65	43217.0
66	38825.0
67	34433.0
68	34433.0
69	29414.0
70	24395.0
71	21208.5
72	18022.0
73	14103.0
74	10184.0
75	10184.0
76	8361.5
77	6539.0
78	5499.5
79	4460.0
80	3912.0
81	3364.0
82	3364.0
83	2734.0
84	2104.0
85	1794.5
86	1485.0
87	1221.0
88	957.0
89	957.0
90	701.0
91	445.0
92	272.0
93	99.0
94	65.0
95	31.0
96	31.0
97	21.5
98	12.0
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	757623.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.57991866770642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.46933976602534	56.61563924478964
2	5.373147253275115	6.725022361991338
3	1.4953541271844248	2.8073741897586135
4	0.6393323688128524	1.6003747056776156
5	0.38350962387605	1.2000000535222943
6	0.2518021986140557	0.9454656665770358
7	0.1791479606342431	0.7847745364183484
8	0.14476740131709565	0.7247625760127249
9	0.09293269080187355	0.5234148208757121
>10	0.7699257859497094	9.638164621166535
>50	0.1041742133479865	4.539509395440585
>100	0.09043875087349039	11.284389359151357
>500	0.005916553795071998	2.4397321607417144
>1k	2.1130549268114283E-4	0.17137630787649116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1296	0.1710613326152981	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	991	0.130803843072346	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	927	0.12235636985677573	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	925	0.12209238631878917	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	825	0.1088932094194606	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	803	0.10598939050160833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3199176899328558E-4
2	0.0	1.3199176899328558E-4	0.0	0.0	1.3199176899328558E-4
3	0.0	1.3199176899328558E-4	0.0	0.0	1.3199176899328558E-4
4	0.0	1.3199176899328558E-4	0.0	0.0	1.3199176899328558E-4
5	0.0	2.6398353798657117E-4	0.0	0.0	1.3199176899328558E-4
6	0.0	2.6398353798657117E-4	0.0	0.0	1.3199176899328558E-4
7	0.0	2.6398353798657117E-4	0.0	0.0	1.3199176899328558E-4
8	0.0	2.6398353798657117E-4	0.0	0.0	1.3199176899328558E-4
9	0.0	2.6398353798657117E-4	0.0	1.3199176899328558E-4	1.3199176899328558E-4
10	0.0	2.6398353798657117E-4	0.0	1.3199176899328558E-4	1.3199176899328558E-4
11	0.0	2.6398353798657117E-4	0.0	1.3199176899328558E-4	1.3199176899328558E-4
12	0.0	2.6398353798657117E-4	0.0	1.3199176899328558E-4	1.3199176899328558E-4
13	0.0	2.6398353798657117E-4	0.0	1.3199176899328558E-4	1.3199176899328558E-4
14	0.0	2.6398353798657117E-4	0.0	2.6398353798657117E-4	1.3199176899328558E-4
15	0.0	2.6398353798657117E-4	0.0	6.599588449664279E-4	1.3199176899328558E-4
16	0.0	3.9597530697985675E-4	0.0	7.919506139597135E-4	1.3199176899328558E-4
17	0.0	3.9597530697985675E-4	0.0	0.0011879259209395703	1.3199176899328558E-4
18	0.0	3.9597530697985675E-4	0.0	0.0013199176899328557	1.3199176899328558E-4
19	0.0	3.9597530697985675E-4	0.0	0.001583901227919427	1.3199176899328558E-4
20	0.0	3.9597530697985675E-4	0.0	0.0019798765348992835	1.3199176899328558E-4
21	0.0	3.9597530697985675E-4	0.0	0.0022438600728858548	1.3199176899328558E-4
22	0.0	3.9597530697985675E-4	0.0	0.003431785993825425	1.3199176899328558E-4
23	0.0	5.279670759731423E-4	0.0	0.005015687221744852	1.3199176899328558E-4
24	0.0	6.599588449664279E-4	0.0	0.0071275555256374214	1.3199176899328558E-4
25	0.0	6.599588449664279E-4	0.0	0.00923942382952999	1.3199176899328558E-4
26	0.0	6.599588449664279E-4	0.0	0.01491506989624127	1.3199176899328558E-4
27	0.0	6.599588449664279E-4	0.0	0.04329330022979767	1.3199176899328558E-4
28	0.0	6.599588449664279E-4	0.0	0.17924482229288183	1.3199176899328558E-4
29	0.0	7.919506139597135E-4	0.0	0.36495724126643464	1.3199176899328558E-4
30	0.0	7.919506139597135E-4	0.0	0.6042583184512614	1.3199176899328558E-4
31	0.0	7.919506139597135E-4	0.0	1.277020365010038	1.3199176899328558E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	630	0.0	24.666668	1
TAAACTA	65	2.680843E-6	22.76923	5
CTTATAC	1055	0.0	20.34123	37
ATACACA	210	0.0	20.261906	37
CTATGCT	55	5.1408686E-4	20.181818	4
CCCAATA	65	6.898163E-5	19.923077	2
CGAACGA	195	0.0	18.974358	16
TAATATG	50	0.007032722	18.5	4
CTAGAAC	60	9.2328567E-4	18.5	3
GCTATTC	50	0.007032722	18.5	3
CTAAACT	80	1.6154852E-5	18.5	4
GTACTAT	70	1.2185014E-4	18.5	1
TCGGTTG	80	1.6154852E-5	18.5	32
ATTTCGG	80	1.6154852E-5	18.5	29
TTTAGAG	105	4.7919275E-7	17.61905	3
TAACGAA	200	0.0	17.575	13
TTTCGGT	85	2.7211318E-5	17.411764	30
GTCCTAA	150	2.5102054E-10	17.266666	1
GTGTACA	75	2.0660947E-4	17.266666	1
ATAACGA	215	0.0	17.209303	12
>>END_MODULE