FastQCFastQC Report
Fri 10 Feb 2017
ERR1631304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345197
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13540.39223979350921356No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12150.35197293139859265No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10470.30330506927928114No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8870.2569547244037463No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC8030.23262079334409047No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7730.22393010367992772No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT6430.18627044846855564No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC6330.1833735519138347No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG6320.18308386225836262No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG5650.1636746553417324No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT5320.15411489671115333No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC5300.15353551740020915No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG5030.14571389670246265No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC4940.1431066898032138No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG4860.14078917255943707No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC4710.1364438277273557No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT4680.1355747587609394No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC4640.13441600013905103No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG4530.131229413928858No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC4390.12717375875224873No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA4200.12166965529827894No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG4030.11674493115525339No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG3910.11326865528958827No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC3890.11268927597864407No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC3870.1121098966676999No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG3870.1121098966676999No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC3850.1115305173567557No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA3770.10921300011297896No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT3670.10631610355825805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.10602641390278596No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT3630.10515734493636968No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3610.10457796562542548No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC3560.10312951734806503No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC3550.10283982769259292No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA3530.10226044838164874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG200.001840152737.03
CGGTAGT303.5943082E-430.83333218
CAATCGG303.5943082E-430.83333214
TTGTGTC250.00549108329.5999989
TCACTAA250.00549108329.5999981
GTATATA250.00549108329.5999981
GACATGT250.00549108329.5999987
ATCGGTA250.00549108329.59999816
GGTAGTA405.928278E-527.7519
ACTAAAC358.856776E-426.428573
TAGCGAC400.001928465623.12524
TGTATGC400.001928465623.1253
GTGTATG400.001928465623.1252
GACGGAC903.805326E-922.611117
GTTCTAG450.00382046820.5555551
AGACCCT450.00382046820.5555556
GCATCGG450.00382046820.5555558
GGTATCA3350.020.4328371
GCGCAAG1101.7425918E-920.1818181
AGTCGGT1750.020.08571411
Produced by FastQC (version 0.11.5)