##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631304.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345197
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.954573765125424	34.0	33.0	34.0	31.0	34.0
2	33.110641170114455	34.0	33.0	34.0	31.0	34.0
3	33.19413552261462	34.0	33.0	34.0	31.0	34.0
4	36.455255984264056	37.0	37.0	37.0	35.0	37.0
5	36.429702459754864	37.0	37.0	37.0	35.0	37.0
6	36.48024171704853	37.0	37.0	37.0	35.0	37.0
7	36.48590804670956	37.0	37.0	37.0	35.0	37.0
8	36.48569367636451	37.0	37.0	37.0	35.0	37.0
9	38.31937994826143	39.0	39.0	39.0	37.0	39.0
10	38.257565969576795	39.0	39.0	39.0	37.0	39.0
11	38.32530699861238	39.0	39.0	39.0	37.0	39.0
12	38.2758251085612	39.0	39.0	39.0	37.0	39.0
13	38.30768807376657	39.0	39.0	39.0	37.0	39.0
14	39.81627302670649	41.0	40.0	41.0	38.0	41.0
15	39.80337024945176	41.0	40.0	41.0	38.0	41.0
16	39.77932890494413	41.0	40.0	41.0	38.0	41.0
17	39.75436345043555	41.0	40.0	41.0	38.0	41.0
18	39.729803561444626	41.0	40.0	41.0	38.0	41.0
19	39.744887701805055	41.0	40.0	41.0	38.0	41.0
20	39.73305387937902	41.0	40.0	41.0	38.0	41.0
21	39.70484969452226	41.0	40.0	41.0	38.0	41.0
22	39.63597018514066	41.0	40.0	41.0	37.0	41.0
23	39.563136411961864	41.0	40.0	41.0	37.0	41.0
24	39.53360544848304	41.0	39.0	41.0	37.0	41.0
25	39.5210937522632	41.0	39.0	41.0	37.0	41.0
26	39.44715046770395	41.0	39.0	41.0	37.0	41.0
27	39.37633872832035	41.0	39.0	41.0	36.0	41.0
28	39.26566858924035	41.0	39.0	41.0	36.0	41.0
29	39.20945141469943	41.0	39.0	41.0	36.0	41.0
30	39.14505630118454	41.0	39.0	41.0	35.0	41.0
31	39.11719684701779	40.0	39.0	41.0	35.0	41.0
32	39.05040600005214	40.0	39.0	41.0	35.0	41.0
33	38.95743589892149	40.0	39.0	41.0	35.0	41.0
34	38.88902568678175	40.0	38.0	41.0	35.0	41.0
35	38.78174491667077	40.0	38.0	41.0	35.0	41.0
36	38.71942398108906	40.0	38.0	41.0	35.0	41.0
37	38.65170902412246	40.0	38.0	41.0	35.0	41.0
38	38.573475435765666	40.0	38.0	41.0	35.0	41.0
39	38.47472312911178	40.0	38.0	41.0	35.0	41.0
40	38.36639368244799	40.0	38.0	41.0	35.0	41.0
41	38.26641309165491	40.0	37.0	41.0	34.0	41.0
42	38.10893489804373	40.0	37.0	41.0	34.0	41.0
43	37.22653441368262	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	1.0
16	1.0
17	3.0
18	7.0
19	13.0
20	15.0
21	48.0
22	91.0
23	151.0
24	221.0
25	326.0
26	489.0
27	714.0
28	1022.0
29	1246.0
30	1743.0
31	2342.0
32	3107.0
33	4250.0
34	6706.0
35	10271.0
36	17267.0
37	32646.0
38	80878.0
39	181637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.150441631879765	16.71741063798353	11.540946184352704	27.591201545784
2	21.605344194764147	18.676581777941294	30.75142599732906	28.966648029965498
3	21.31913081515772	19.78145812391185	27.792825545992578	31.106585514937844
4	14.8118320842881	14.635700773761071	32.16163524016721	38.39083190178362
5	17.454670811160003	34.17787524225297	31.495059342925924	16.872394603661096
6	37.567533900931934	32.25868127475036	14.804879532556772	15.368905291760937
7	32.32183361964328	27.864378890894187	19.034927881760268	20.77885960770227
8	30.87309565262734	28.711141753839115	18.168176432587767	22.24758616094578
9	27.911308615080664	12.988815082402223	17.633699018241757	41.46617728427536
10	19.673983261731706	23.416483920775676	29.35888782347471	27.550644994017908
11	40.77294993873064	19.594318606476882	18.15485070843605	21.47788074635643
12	24.994713163787637	22.920245540951978	25.146800232910483	26.938241062349906
13	33.099070965274905	16.14846015463634	22.99411640309736	27.758352476991398
14	24.665046335860392	18.51841122605353	20.950066194086276	35.8664762439998
15	28.83657737465853	24.802069542898693	19.15254188188194	27.208811200560838
16	29.320938478607868	22.72962974765134	20.682682642085535	27.26674913165526
17	27.828457373615645	23.03380388589704	21.20151681503605	27.936221925451264
18	28.379736787979038	20.197162779514304	23.33479143793254	28.088308994574113
19	27.756034959747623	22.60506319579834	21.420811884228428	28.218089960225612
20	27.962293994443755	20.729902055927486	22.152857643606406	29.154946306022357
21	30.512142341909115	21.665309953446872	21.71021185004505	26.112335854598967
22	30.36671813486212	20.844908849149906	20.917910642328874	27.870462373659098
23	28.04601430487519	20.4370258142452	22.149091678085266	29.367868202794345
24	26.72879544144358	22.242661436802752	21.954709919263493	29.073833202490174
25	27.913336442668967	21.286395884089377	21.697175815548803	29.103091857692853
26	28.52139502950489	22.49527081637442	21.419653125606537	27.563681028514154
27	29.357149685541877	20.678916676564395	22.113170160806728	27.850763477087
28	27.55875630437113	21.366639918655146	22.487159506021197	28.587444270952528
29	26.297157854790164	21.685008850018974	23.563356576100027	28.454476719090838
30	25.37015095727947	23.4089519897334	23.99267664550967	27.228220407477473
31	28.467223063931613	22.609408540630422	21.20673122883455	27.71663716660342
32	27.172310303971354	21.46426533254924	22.258594367853718	29.104829995625686
33	26.307586682387157	21.06130702178756	22.853037540882454	29.778068754942833
34	27.734308235587214	21.286685573744847	22.990640127231696	27.988366063436242
35	27.375672442112766	20.826658400855163	24.050904266259558	27.746764890772514
36	25.66737254379383	22.742665782147586	23.584503920949487	28.00545775310909
37	27.68477130450149	20.634014779966222	24.333061990689377	27.348151924842917
38	25.64361799204512	20.03030153796238	25.324379991714874	29.00170047827762
39	26.626534993061934	19.89298864126861	25.50456695741852	27.975909408250942
40	25.60595833683375	20.28522843477782	25.737767130073554	28.371046098314874
41	24.27019933545193	20.79682036634154	27.50371526983143	27.4292650283751
42	25.62391909547302	20.36373433141076	27.55093468367338	26.461411889442836
43	22.992957644475474	19.672534813454345	27.575558304388508	29.75894923768167
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	5.0
2	5.0
3	21.0
4	37.0
5	37.0
6	52.5
7	68.0
8	64.5
9	61.0
10	93.5
11	126.0
12	126.0
13	206.0
14	286.0
15	482.0
16	678.0
17	614.0
18	550.0
19	550.0
20	618.5
21	687.0
22	597.5
23	508.0
24	576.5
25	645.0
26	645.0
27	781.5
28	918.0
29	1233.0
30	1548.0
31	1995.5
32	2443.0
33	2443.0
34	3305.0
35	4167.0
36	5513.0
37	6859.0
38	8395.5
39	9932.0
40	9932.0
41	11295.0
42	12658.0
43	14085.5
44	15513.0
45	17505.0
46	19497.0
47	19497.0
48	22170.5
49	24844.0
50	25812.5
51	26781.0
52	28572.5
53	30364.0
54	30364.0
55	29507.0
56	28650.0
57	28131.5
58	27613.0
59	27778.0
60	27943.0
61	27943.0
62	27116.0
63	26289.0
64	25060.0
65	23831.0
66	21744.0
67	19657.0
68	19657.0
69	16116.5
70	12576.0
71	10640.0
72	8704.0
73	6314.5
74	3925.0
75	3925.0
76	3033.0
77	2141.0
78	1825.5
79	1510.0
80	1399.0
81	1288.0
82	1288.0
83	998.5
84	709.0
85	619.5
86	530.0
87	458.0
88	386.0
89	386.0
90	296.5
91	207.0
92	124.0
93	41.0
94	26.5
95	12.0
96	12.0
97	8.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	345197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.07820029934226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.44337245286135	44.153718926978975
2	6.518065086605238	6.658620681155439
3	2.1840907903298965	3.346782805812576
4	1.1318173666405802	2.312447766221666
5	0.6822041522708386	1.7422880167366441
6	0.43003025576646026	1.317910292328998
7	0.34659134930168456	1.2392283653155518
8	0.25932078070035663	1.0596511022715698
9	0.20876272594392412	0.9596901897720405
>10	1.4778740132167492	15.08147450186446
>50	0.1805826440090684	6.524482175750214
>100	0.12988278496551292	12.659933772676094
>500	0.005696613375680392	1.8916139064599804
>1k	0.0017089840127041175	1.0521574966557896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1354	0.39223979350921356	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1215	0.35197293139859265	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1047	0.30330506927928114	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	887	0.2569547244037463	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	803	0.23262079334409047	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	773	0.22393010367992772	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	643	0.18627044846855564	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	633	0.1833735519138347	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	632	0.18308386225836262	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	565	0.1636746553417324	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	532	0.15411489671115333	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	530	0.15353551740020915	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	503	0.14571389670246265	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	494	0.1431066898032138	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	486	0.14078917255943707	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	471	0.1364438277273557	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	468	0.1355747587609394	No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC	464	0.13441600013905103	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	453	0.131229413928858	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	439	0.12717375875224873	No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA	420	0.12166965529827894	No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG	403	0.11674493115525339	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	391	0.11326865528958827	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	389	0.11268927597864407	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	387	0.1121098966676999	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	387	0.1121098966676999	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC	385	0.1115305173567557	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	377	0.10921300011297896	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	367	0.10631610355825805	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	366	0.10602641390278596	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	363	0.10515734493636968	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	361	0.10457796562542548	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	356	0.10312951734806503	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	355	0.10283982769259292	No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA	353	0.10226044838164874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	2.8968965547209277E-4	0.0
15	0.0	0.0	0.0	8.690689664162782E-4	0.0
16	0.0	0.0	0.0	0.0017381379328325565	0.0
17	0.0	0.0	0.0	0.002027827588304649	0.0
18	0.0	0.0	0.0	0.0026072068992488346	0.0
19	0.0	0.0	0.0	0.00318658621019302	0.0
20	0.0	0.0	0.0	0.003765965521137206	0.0
21	0.0	0.0	0.0	0.006083482764913948	0.0
22	0.0	0.0	0.0	0.008690689664162782	0.0
23	0.0	0.0	0.0	0.011297896563411618	0.0
24	0.0	0.0	0.0	0.016222620706437194	0.0
25	0.0	0.0	0.0	0.019409206916630214	0.0
26	0.0	0.0	0.0	0.024623620715127883	0.0
27	0.0	0.0	0.0	0.048088482808367396	0.0
28	0.0	2.8968965547209277E-4	0.0	0.14252731049226963	0.0
29	0.0	2.8968965547209277E-4	0.0	0.3035947589347532	0.0
30	0.0	2.8968965547209277E-4	0.0	0.5367949315897879	0.0
31	0.0	2.8968965547209277E-4	0.0	1.1040072770041454	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATAGG	20	0.0018401527	37.0	3
CGGTAGT	30	3.5943082E-4	30.833332	18
CAATCGG	30	3.5943082E-4	30.833332	14
TTGTGTC	25	0.005491083	29.599998	9
TCACTAA	25	0.005491083	29.599998	1
GTATATA	25	0.005491083	29.599998	1
GACATGT	25	0.005491083	29.599998	7
ATCGGTA	25	0.005491083	29.599998	16
GGTAGTA	40	5.928278E-5	27.75	19
ACTAAAC	35	8.856776E-4	26.42857	3
TAGCGAC	40	0.0019284656	23.125	24
TGTATGC	40	0.0019284656	23.125	3
GTGTATG	40	0.0019284656	23.125	2
GACGGAC	90	3.805326E-9	22.61111	7
GTTCTAG	45	0.003820468	20.555555	1
AGACCCT	45	0.003820468	20.555555	6
GCATCGG	45	0.003820468	20.555555	8
GGTATCA	335	0.0	20.432837	1
GCGCAAG	110	1.7425918E-9	20.181818	1
AGTCGGT	175	0.0	20.085714	11
>>END_MODULE