FastQCFastQC Report
Fri 10 Feb 2017
ERR1631303.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631303.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719917
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.3229538960741308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20210.28072680600680355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16470.22877637283186814No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC11660.1619631151924458No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10300.14307204858337838No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT10210.14182190446954301No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8930.12404207707277368No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC8270.11487435357131447No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8050.11181844573749473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.10390086634987088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAACC250.00549499629.63
CCGAAAT400.001930490123.12524
TACCGTC1059.804353E-1021.1428577
ATACCGT1101.7516868E-920.1818186
GGTATCA12400.020.141131
AGCGTCA1052.2559107E-819.380953
TGCATCA4050.019.18518414
GCTGCAT4100.018.95121812
GACAGGC4250.018.7176467
AACCGAA609.232247E-418.522
TAGCGCC609.232247E-418.529
CGTCGTA1205.164111E-918.510
CTGCATC4650.017.90322513
ACAGGCT4450.017.8764068
GCATCAG4700.017.31914715
ATACACA3850.017.29870237
ACCGAAA650.001579158817.07692323
ACCGTCG1301.3904355E-817.0769238
TCCAGGA4850.016.7835042
CTTATAC12900.016.63565837
Produced by FastQC (version 0.11.5)