##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631303.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	719917
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04698597199399	34.0	33.0	34.0	31.0	34.0
2	33.1933403434007	34.0	33.0	34.0	31.0	34.0
3	33.26751694987061	34.0	34.0	34.0	31.0	34.0
4	36.48846186435381	37.0	37.0	37.0	35.0	37.0
5	36.48533928216725	37.0	37.0	37.0	35.0	37.0
6	36.534843600026115	37.0	37.0	37.0	35.0	37.0
7	36.543295963284656	37.0	37.0	37.0	35.0	37.0
8	36.542245842229036	37.0	37.0	37.0	35.0	37.0
9	38.391524300718	39.0	39.0	39.0	37.0	39.0
10	38.36130137224152	39.0	39.0	39.0	37.0	39.0
11	38.4076719955217	39.0	39.0	39.0	37.0	39.0
12	38.36904809860025	39.0	39.0	39.0	37.0	39.0
13	38.39684435844688	39.0	39.0	39.0	37.0	39.0
14	39.93256861554874	41.0	40.0	41.0	38.0	41.0
15	39.926534586625955	41.0	40.0	41.0	38.0	41.0
16	39.88596463203397	41.0	40.0	41.0	38.0	41.0
17	39.87614405549529	41.0	40.0	41.0	38.0	41.0
18	39.82058209488038	41.0	40.0	41.0	38.0	41.0
19	39.84176648141383	41.0	40.0	41.0	38.0	41.0
20	39.81143659616317	41.0	40.0	41.0	38.0	41.0
21	39.78698099919852	41.0	40.0	41.0	38.0	41.0
22	39.72024136115691	41.0	40.0	41.0	37.0	41.0
23	39.64915677779522	41.0	40.0	41.0	37.0	41.0
24	39.621406356566105	41.0	40.0	41.0	37.0	41.0
25	39.60284449457368	41.0	40.0	41.0	37.0	41.0
26	39.4961780316342	41.0	40.0	41.0	37.0	41.0
27	39.43843943121221	41.0	39.0	41.0	36.0	41.0
28	39.352900403796546	41.0	39.0	41.0	36.0	41.0
29	39.29009177446844	41.0	39.0	41.0	35.0	41.0
30	39.21765147926775	41.0	39.0	41.0	35.0	41.0
31	39.16440228526344	41.0	39.0	41.0	35.0	41.0
32	39.08465975938893	41.0	39.0	41.0	35.0	41.0
33	38.98624146950274	41.0	39.0	41.0	35.0	41.0
34	38.91666678242075	41.0	39.0	41.0	35.0	41.0
35	38.79529862470257	40.0	38.0	41.0	35.0	41.0
36	38.73639322310766	40.0	38.0	41.0	35.0	41.0
37	38.66035390190814	40.0	38.0	41.0	35.0	41.0
38	38.57332164680095	40.0	38.0	41.0	35.0	41.0
39	38.47185578337503	40.0	38.0	41.0	35.0	41.0
40	38.34883465732855	40.0	37.0	41.0	35.0	41.0
41	38.23855666694911	40.0	37.0	41.0	35.0	41.0
42	38.08731562110632	40.0	37.0	41.0	34.0	41.0
43	37.25767137044965	40.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	2.0
16	0.0
17	3.0
18	10.0
19	24.0
20	47.0
21	109.0
22	182.0
23	302.0
24	459.0
25	698.0
26	1119.0
27	1504.0
28	2131.0
29	2954.0
30	3914.0
31	5161.0
32	6664.0
33	8699.0
34	13808.0
35	21681.0
36	33408.0
37	64417.0
38	139056.0
39	413563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6511431178872	17.11502853801202	12.02277484765605	27.21105349644473
2	20.669188253645906	18.756189949674752	32.561392493856935	28.013229302822406
3	21.477059160986613	20.41596461814348	27.615405664819693	30.491570556050213
4	15.867801427108958	15.05242965508524	31.30958152120314	37.77018739660266
5	17.58924987186023	33.64485072584756	30.747433384681845	18.01846601761036
6	36.243761433609706	31.584335416443842	15.334962224812026	16.836940925134424
7	31.490713512807726	28.20533478164844	19.19804644146478	21.105905264079052
8	29.508540567871016	30.193758447154323	18.53435882191975	21.763342163054908
9	27.93613708246923	13.777004849170114	18.704795136105968	39.58206293225469
10	20.744613615180636	23.77774104514826	28.712893291865587	26.764752047805512
11	38.380535533957385	20.497223985542778	18.924542690337915	22.19769779016192
12	24.66964941791901	23.88455891443041	25.26791282883999	26.177878838810585
13	32.06550199536891	17.178646982916085	23.477706457827775	27.278144563887224
14	24.61492088671333	19.42779514860741	22.278540442856606	33.67874352182266
15	28.337155533207298	24.831751438012994	19.959245301888966	26.871847726890742
16	27.578734770813856	23.45978772552947	21.74611795526429	27.21535954839238
17	26.342064432427627	24.218069583021375	22.777070134473835	26.66279585007716
18	26.391792387177965	21.70277962598466	24.503658060581984	27.401769926255387
19	26.669741095154027	23.493541616603025	23.61091625840201	26.22580102984094
20	26.938105364924013	22.268956004650537	23.69120329149055	27.10173533893491
21	29.351439124232375	22.4056384277632	22.900695496841998	25.342226951162427
22	28.72414458889011	22.24992603313993	22.408555430695483	26.617373947274476
23	27.01228058234491	22.42744649730455	23.133222302015373	27.42705061833517
24	26.650572218741882	23.210175617467012	23.29643556132165	26.842816602469448
25	26.883099023915257	22.129356578605588	23.32796697397061	27.65957742350854
26	27.07200969000593	23.34421884745047	23.60994392409125	25.973827538452348
27	27.884186649294296	22.082545626787535	23.050434980699166	26.98283274321901
28	26.045502467645576	22.465089725621148	24.424065552001135	27.065342254732144
29	25.774637909647918	22.6744194122378	24.78424596168725	26.766696716427035
30	25.28874856407058	23.285323169198673	24.956626944494992	26.469301322235754
31	26.484164146700245	23.12322114910469	23.293657463290906	27.09895724090416
32	25.536554908413052	22.57593583704788	24.14431108030509	27.743198174233974
33	25.662680559008887	22.32604591918235	24.39906266972443	27.61221085208434
34	26.709884611698293	22.321600962333157	24.27363154363628	26.694882882332273
35	26.482497287881795	22.136579633485525	25.241798707350988	26.139124371281692
36	25.071501298066305	22.676225175957786	24.679650570829693	27.57262295514622
37	25.36792435794682	21.39566088868578	25.722270761768375	27.51414399159903
38	24.881896107467945	20.676411308525843	26.920047727724167	27.521644856282045
39	24.46976526460689	20.839902377635198	27.245224102222892	27.44510825553501
40	24.277520880879326	20.787951944460264	27.9293307422939	27.005196432366507
41	22.966814229973735	21.036591718211962	29.099465632843785	26.897128418970524
42	23.375889165000967	21.57109777932734	28.46897628476616	26.584036770905534
43	22.003230928009756	20.56417614808374	29.1766967580985	28.255896165808004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	91.0
2	71.0
3	177.0
4	283.0
5	283.0
6	345.0
7	407.0
8	429.0
9	451.0
10	665.5
11	880.0
12	880.0
13	1644.0
14	2408.0
15	3574.0
16	4740.0
17	4228.5
18	3717.0
19	3717.0
20	4291.0
21	4865.0
22	4008.5
23	3152.0
24	3446.5
25	3741.0
26	3741.0
27	4288.0
28	4835.0
29	5960.5
30	7086.0
31	8588.0
32	10090.0
33	10090.0
34	12373.0
35	14656.0
36	17788.0
37	20920.0
38	23685.0
39	26450.0
40	26450.0
41	28938.5
42	31427.0
43	33908.0
44	36389.0
45	38708.5
46	41028.0
47	41028.0
48	44229.0
49	47430.0
50	49675.0
51	51920.0
52	53856.5
53	55793.0
54	55793.0
55	55131.5
56	54470.0
57	52649.5
58	50829.0
59	48976.0
60	47123.0
61	47123.0
62	45646.5
63	44170.0
64	41101.5
65	38033.0
66	34141.5
67	30250.0
68	30250.0
69	26488.0
70	22726.0
71	20405.0
72	18084.0
73	16283.0
74	14482.0
75	14482.0
76	12822.5
77	11163.0
78	9156.0
79	7149.0
80	5245.0
81	3341.0
82	3341.0
83	2680.5
84	2020.0
85	1759.5
86	1499.0
87	1300.5
88	1102.0
89	1102.0
90	802.5
91	503.0
92	289.5
93	76.0
94	57.5
95	39.0
96	39.0
97	21.5
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	719917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.901791031383794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.87148807878698	48.7920386714968
2	5.675703419147384	6.232125661482804
3	1.6890292055724683	2.7819218547073152
4	0.8340736789430261	1.8316855530443006
5	0.5010896334926314	1.3755359173002575
6	0.35566344632649866	1.171593612463153
7	0.25016827714875567	0.9614280532291605
8	0.20557345404256439	0.9029080652355722
9	0.15190223708436684	0.7505734389845063
>10	1.1683090496549458	12.719486374022605
>50	0.15750363049543756	6.105557260228067
>100	0.13340879595343083	13.552334347630527
>500	0.004565320013609898	1.540348134654515
>1k	0.001521773337869966	1.2824630555205283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2325	0.3229538960741308	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2021	0.28072680600680355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1647	0.22877637283186814	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1166	0.1619631151924458	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1030	0.14307204858337838	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1021	0.14182190446954301	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	893	0.12404207707277368	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	827	0.11487435357131447	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	805	0.11181844573749473	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	748	0.10390086634987088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.3890490153726056E-4	0.0
12	0.0	0.0	0.0	1.3890490153726056E-4	0.0
13	0.0	0.0	0.0	2.778098030745211E-4	0.0
14	0.0	0.0	0.0	2.778098030745211E-4	0.0
15	1.3890490153726056E-4	0.0	0.0	2.778098030745211E-4	0.0
16	1.3890490153726056E-4	0.0	0.0	0.001250144113835345	0.0
17	1.3890490153726056E-4	0.0	0.0	0.002361383326133429	0.0
18	1.3890490153726056E-4	0.0	0.0	0.002361383326133429	0.0
19	1.3890490153726056E-4	0.0	0.0	0.0027780980307452107	0.0
20	1.3890490153726056E-4	0.0	0.0	0.0031948127353569927	0.0
21	1.3890490153726056E-4	0.0	0.0	0.0037504323415060345	0.0
22	1.3890490153726056E-4	0.0	0.0	0.004583861750729598	0.0
23	1.3890490153726056E-4	0.0	0.0	0.006945245076863027	0.0
24	1.3890490153726056E-4	0.0	0.0	0.013195965646039752	0.0
25	1.3890490153726056E-4	0.0	0.0	0.016946397987545787	0.0
26	2.778098030745211E-4	0.0	0.0	0.029725648928973756	0.0
27	2.778098030745211E-4	0.0	0.0	0.08751008796847415	1.3890490153726056E-4
28	4.167147046117816E-4	0.0	0.0	0.27405937073301506	1.3890490153726056E-4
29	4.167147046117816E-4	0.0	0.0	0.5071417955125382	2.778098030745211E-4
30	4.167147046117816E-4	0.0	0.0	0.8214835876913589	2.778098030745211E-4
31	4.167147046117816E-4	0.0	0.0	1.5217031963406893	2.778098030745211E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAACC	25	0.005494996	29.6	3
CCGAAAT	40	0.0019304901	23.125	24
TACCGTC	105	9.804353E-10	21.142857	7
ATACCGT	110	1.7516868E-9	20.181818	6
GGTATCA	1240	0.0	20.14113	1
AGCGTCA	105	2.2559107E-8	19.38095	3
TGCATCA	405	0.0	19.185184	14
GCTGCAT	410	0.0	18.951218	12
GACAGGC	425	0.0	18.717646	7
AACCGAA	60	9.232247E-4	18.5	22
TAGCGCC	60	9.232247E-4	18.5	29
CGTCGTA	120	5.164111E-9	18.5	10
CTGCATC	465	0.0	17.903225	13
ACAGGCT	445	0.0	17.876406	8
GCATCAG	470	0.0	17.319147	15
ATACACA	385	0.0	17.298702	37
ACCGAAA	65	0.0015791588	17.076923	23
ACCGTCG	130	1.3904355E-8	17.076923	8
TCCAGGA	485	0.0	16.783504	2
CTTATAC	1290	0.0	16.635658	37
>>END_MODULE