FastQCFastQC Report
Fri 10 Feb 2017
ERR1631302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202304
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46040.38293143830512083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38450.3198026455871394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34910.2903591770467369No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA22190.18456230703715532No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC19270.16027560417332054No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC15300.12725566911529862No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC14950.12434459171723625No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14860.12359602895773449No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14820.12326333439795592No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14360.11943734696050251No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT13560.11278345576493133No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.1122844139252635No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG13030.10837525284786544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATA701.923072E-723.7857131
CGTGAAT855.1812094E-821.76470610
TCTAGAC2600.021.3461533
TCACGGC450.00382590920.5555553
CTAGACA2900.020.4137944
ACATGTA2200.020.1818188
GGTATCA23700.019.5928271
AGTCGGT7550.018.62251711
TTTCCGA609.237151E-418.517
GCAGTCG7800.018.2628219
GTTCTAG3250.018.2153851
TAGAACT3250.018.2153854
ATACACA4150.017.83132637
GTCTAGA2200.017.659091
ACAACGG1705.456968E-1217.41176423
GGCAGTC8300.017.3855428
CGCCTTA6550.016.94656625
CTCTATG11450.016.8034931
TATACAC5950.016.78991737
CGGAGTC1554.0017767E-1016.70967727
Produced by FastQC (version 0.11.5)