##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631302.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1202304
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.882721008996064	34.0	31.0	34.0	31.0	34.0
2	33.029035085968275	34.0	33.0	34.0	31.0	34.0
3	33.1155356715107	34.0	33.0	34.0	31.0	34.0
4	36.4157068428617	37.0	37.0	37.0	35.0	37.0
5	36.363949550196956	37.0	37.0	37.0	35.0	37.0
6	36.418804229213244	37.0	37.0	37.0	35.0	37.0
7	36.42825940860215	37.0	37.0	37.0	35.0	37.0
8	36.4230835129884	37.0	37.0	37.0	35.0	37.0
9	38.225872990524856	39.0	39.0	39.0	37.0	39.0
10	38.164662181944	39.0	38.0	39.0	37.0	39.0
11	38.22773524832322	39.0	38.0	39.0	37.0	39.0
12	38.183015277334185	39.0	38.0	39.0	37.0	39.0
13	38.21386853907165	39.0	38.0	39.0	37.0	39.0
14	39.68390606701799	41.0	40.0	41.0	38.0	41.0
15	39.65649286702864	41.0	40.0	41.0	37.0	41.0
16	39.626243445917176	41.0	40.0	41.0	37.0	41.0
17	39.61846088842755	41.0	40.0	41.0	37.0	41.0
18	39.60092954860002	41.0	40.0	41.0	37.0	41.0
19	39.61320764132865	41.0	40.0	41.0	37.0	41.0
20	39.58967948206111	41.0	40.0	41.0	37.0	41.0
21	39.555426913659105	41.0	40.0	41.0	37.0	41.0
22	39.500245362237834	41.0	39.0	41.0	37.0	41.0
23	39.428513088203985	41.0	39.0	41.0	37.0	41.0
24	39.39451004072181	41.0	39.0	41.0	37.0	41.0
25	39.39296716970084	41.0	39.0	41.0	36.0	41.0
26	39.28788309778559	41.0	39.0	41.0	36.0	41.0
27	39.23412547908016	40.0	39.0	41.0	36.0	41.0
28	39.15067154396891	40.0	39.0	41.0	36.0	41.0
29	39.09355870062813	40.0	39.0	41.0	35.0	41.0
30	39.01520663659108	40.0	39.0	41.0	35.0	41.0
31	38.96092835089961	40.0	39.0	41.0	35.0	41.0
32	38.872438251889704	40.0	38.0	41.0	35.0	41.0
33	38.780095549877565	40.0	38.0	41.0	35.0	41.0
34	38.715755748961996	40.0	38.0	41.0	35.0	41.0
35	38.613572773608006	40.0	38.0	41.0	35.0	41.0
36	38.53604080166081	40.0	38.0	41.0	35.0	41.0
37	38.471688524699246	40.0	38.0	41.0	35.0	41.0
38	38.382277693495155	40.0	38.0	41.0	35.0	41.0
39	38.285322181411686	40.0	38.0	41.0	34.0	41.0
40	38.17169700841052	40.0	37.0	41.0	34.0	41.0
41	38.069326892366654	40.0	37.0	41.0	34.0	41.0
42	37.90443099249441	40.0	37.0	41.0	34.0	41.0
43	37.034532863568614	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	6.0
12	1.0
13	4.0
14	10.0
15	6.0
16	17.0
17	16.0
18	29.0
19	49.0
20	89.0
21	171.0
22	337.0
23	538.0
24	866.0
25	1347.0
26	1997.0
27	2952.0
28	4096.0
29	5696.0
30	7769.0
31	9956.0
32	12680.0
33	17016.0
34	24920.0
35	37169.0
36	62476.0
37	126769.0
38	300761.0
39	584560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14915029809433	17.493662168636217	12.063255216650697	26.293932316618758
2	19.650604173320556	20.075288778877887	33.15633982753114	27.117767220270416
3	20.30626197700415	21.515357180879377	28.63202650910252	29.546354333013948
4	14.448425689343129	15.149163605876717	33.087222532737144	37.315188172043015
5	16.199147636537848	35.2943182423081	31.779317044607687	16.727217076546363
6	35.258553577131906	33.482713190673906	15.765896146066218	15.492837086127967
7	31.127568402001494	29.514166134355367	19.787923852869156	19.57034161077398
8	29.466507638667096	30.688411583093796	19.19872178750133	20.646358990737784
9	27.939190088363674	13.643554375598852	18.892642792505058	39.524612743532415
10	19.050173666560205	24.9426101884382	29.81334171723624	26.19387442776536
11	38.260706110933675	21.051497790908122	19.47569000851698	21.21210608964122
12	24.129920552539126	24.93529090812307	26.418859123815608	24.515929415522198
13	31.28534879697647	17.750668716065153	24.65491256786969	26.309069919088685
14	24.191136351538383	19.67073219418716	23.194466624081763	32.943664830192695
15	27.269559113169382	25.992178350899607	20.84905315128287	25.889209384648144
16	26.93752994251038	24.360976924305334	22.73085675503034	25.970636378153944
17	25.42543316831683	25.219578409453845	23.387762163313106	25.967226258916217
18	26.194955685084636	22.463120808048547	25.320052166506972	26.02187134035984
19	25.873156872138825	23.961992973490897	24.504617800489726	25.660232353880545
20	26.23945358245502	22.868093260938995	24.781918716065153	26.110534440540828
21	28.2189030394975	23.249693921005004	24.37345297029703	24.157950069200467
22	27.596597865431704	22.685194426700733	23.58197261258384	26.136235095283723
23	25.769522516767807	22.83823392419887	24.60991562865964	26.782327930373683
24	25.233052539124877	23.886554482061108	24.485820557862237	26.39457242095177
25	25.922229319706165	23.033359283508997	24.49372205365698	26.55068934312786
26	26.22406645906526	24.07444373469605	24.195211859895668	25.50627794634302
27	26.572480836793353	22.83249494304269	24.180157431065687	26.414866789098262
28	25.028528558501012	23.343596960502502	25.35881108272118	26.26906339827531
29	24.872827504524647	23.45488329074843	25.22839481528798	26.44389438943894
30	23.993682130309804	24.47525750558927	26.12475713297136	25.406303231129563
31	26.038173373788993	23.859107180879377	23.923234057276694	26.179485388054935
32	25.087997711061426	23.372208692643458	24.564170126690087	26.975623469605026
33	24.71546297774939	22.815943388693707	25.52382758437134	26.944766049185564
34	26.033515649952093	22.863352363462152	24.909756600660067	26.193375385925687
35	25.54087818055999	22.90202810603641	25.835146518684127	25.721947194719476
36	24.270816698605344	23.50378939103588	25.803790056424997	26.421603853933778
37	25.362969764718407	22.497721042265518	26.046074869583734	26.09323432343234
38	24.23970975726605	21.696010326839136	27.200940860215056	26.863339055679763
39	25.076103880549343	21.639535425316726	26.956659879697646	26.327700814436284
40	24.09706696476099	22.585469232407114	27.155029010965613	26.162434791866286
41	23.344179175982113	22.40548147556691	28.35123230064942	25.899107047801557
42	24.19047096241882	22.367055253912486	28.014462232513576	25.428011551155116
43	22.301348078356224	21.948442324071117	28.19112304375599	27.55908655381667
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	155.0
1	156.0
2	157.0
3	433.5
4	710.0
5	710.0
6	857.5
7	1005.0
8	990.5
9	976.0
10	1452.0
11	1928.0
12	1928.0
13	3131.5
14	4335.0
15	6626.5
16	8918.0
17	8098.0
18	7278.0
19	7278.0
20	7967.0
21	8656.0
22	6766.0
23	4876.0
24	5180.5
25	5485.0
26	5485.0
27	6375.5
28	7266.0
29	9020.0
30	10774.0
31	13075.0
32	15376.0
33	15376.0
34	19665.5
35	23955.0
36	30229.5
37	36504.0
38	42517.5
39	48531.0
40	48531.0
41	53003.5
42	57476.0
43	62389.5
44	67303.0
45	75555.0
46	83807.0
47	83807.0
48	90496.0
49	97185.0
50	98224.5
51	99264.0
52	100072.5
53	100881.0
54	100881.0
55	96336.5
56	91792.0
57	87641.5
58	83491.0
59	81042.5
60	78594.0
61	78594.0
62	73357.5
63	68121.0
64	63346.5
65	58572.0
66	52580.0
67	46588.0
68	46588.0
69	38420.0
70	30252.0
71	25940.5
72	21629.0
73	16239.5
74	10850.0
75	10850.0
76	8302.0
77	5754.0
78	4916.0
79	4078.0
80	3733.5
81	3389.0
82	3389.0
83	2773.0
84	2157.0
85	2037.0
86	1917.0
87	1692.5
88	1468.0
89	1468.0
90	1076.0
91	684.0
92	395.0
93	106.0
94	73.0
95	40.0
96	40.0
97	29.5
98	19.0
99	10.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1202304.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.67083931616674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.69226376598759	37.83274506012842
2	7.354211502337063	6.570376006360082
3	2.4630937433042983	3.3008539448340577
4	1.3053724538104727	2.3324833252767165
5	0.7836475040680724	1.7503095867369989
6	0.5386782030363374	1.44379244705746
7	0.3998252736345324	1.2502371387149416
8	0.2790628477523267	0.9972777304844872
9	0.24356475607692787	0.979221787361441
>10	1.5431995840822035	13.78655657367406
>50	0.20592982491752893	6.431800660059185
>100	0.17134413138512888	15.224453021950199
>500	0.016256204111777237	5.229067910105807
>1k	0.0035502054956754884	2.8708248072561995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4604	0.38293143830512083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3845	0.3198026455871394	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3491	0.2903591770467369	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2219	0.18456230703715532	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1927	0.16027560417332054	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1530	0.12725566911529862	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1495	0.12434459171723625	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1486	0.12359602895773449	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1482	0.12326333439795592	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1436	0.11943734696050251	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1356	0.11278345576493133	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1350	0.1122844139252635	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1303	0.10837525284786544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	8.317363994463962E-5	0.0
12	0.0	0.0	0.0	7.485627595017567E-4	0.0
13	0.0	0.0	0.0	0.0011644309592249548	0.0
14	0.0	0.0	8.317363994463962E-5	0.0018298200787820718	0.0
15	0.0	0.0	8.317363994463962E-5	0.002495209198339189	0.0
16	0.0	0.0	8.317363994463962E-5	0.0038259874374534228	0.0
17	0.0	0.0	8.317363994463962E-5	0.005323112956456936	0.0
18	0.0	0.0	8.317363994463962E-5	0.005738981156180135	0.0
19	0.0	0.0	8.317363994463962E-5	0.00657071755562653	0.0
20	0.0	0.0	8.317363994463962E-5	0.007402453955072927	0.0
21	0.0	0.0	8.317363994463962E-5	0.008899579474076439	0.0
22	0.0	0.0	8.317363994463962E-5	0.012309698711806665	0.0
23	0.0	0.0	8.317363994463962E-5	0.016884248908761845	0.0
24	0.0	0.0	8.317363994463962E-5	0.023787661024166934	0.0
25	0.0	0.0	8.317363994463962E-5	0.027946343021398912	0.0
26	0.0	0.0	8.317363994463962E-5	0.036679575215586076	0.0
27	0.0	0.0	8.317363994463962E-5	0.07310962951133823	0.0
28	0.0	0.0	8.317363994463962E-5	0.21591876929628448	0.0
29	0.0	1.6634727988927925E-4	8.317363994463962E-5	0.40854891940806987	0.0
30	0.0	1.6634727988927925E-4	8.317363994463962E-5	0.6804435483870968	0.0
31	0.0	1.6634727988927925E-4	8.317363994463962E-5	1.2745528585116577	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTATA	70	1.923072E-7	23.785713	1
CGTGAAT	85	5.1812094E-8	21.764706	10
TCTAGAC	260	0.0	21.346153	3
TCACGGC	45	0.003825909	20.555555	3
CTAGACA	290	0.0	20.413794	4
ACATGTA	220	0.0	20.181818	8
GGTATCA	2370	0.0	19.592827	1
AGTCGGT	755	0.0	18.622517	11
TTTCCGA	60	9.237151E-4	18.5	17
GCAGTCG	780	0.0	18.262821	9
GTTCTAG	325	0.0	18.215385	1
TAGAACT	325	0.0	18.215385	4
ATACACA	415	0.0	17.831326	37
GTCTAGA	220	0.0	17.65909	1
ACAACGG	170	5.456968E-12	17.411764	23
GGCAGTC	830	0.0	17.385542	8
CGCCTTA	655	0.0	16.946566	25
CTCTATG	1145	0.0	16.803493	1
TATACAC	595	0.0	16.789917	37
CGGAGTC	155	4.0017767E-10	16.709677	27
>>END_MODULE