FastQCFastQC Report
Fri 10 Feb 2017
ERR1631301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110700
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28322.5582655826558267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27592.4923215898825655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20231.8274616079494126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.7280939476061428No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5950.5374887082204155No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.38482384823848237No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3730.3369467028003613No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.2737127371273713No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2930.2646793134598013No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2610.23577235772357727No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2550.23035230352303526No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA2340.21138211382113822No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA2290.2068654019873532No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.20596205962059622No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2120.1915085817524842No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.18157181571815717No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1880.16982836495031617No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1720.15537488708220415No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1700.15356820234869015No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1670.15085817524841916No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1580.14272809394760613No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1540.13911472448057816No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1530.13821138211382114No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1530.13821138211382114No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.13640469738030714No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1490.13459801264679314No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1440.13008130081300812No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.12827461607949414No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1370.12375790424570912No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.12014453477868113No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.11291779584462511No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10840108401084012No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT1170.10569105691056911No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1170.10569105691056911No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.10388437217705511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG251.2264328E-436.99999625
CATCTCC251.2264328E-436.9999969
CACGAGA251.2264328E-436.99999621
GAGACTA251.2264328E-436.99999624
ATCTCGT303.5786876E-430.83333437
ATGATCT303.5786876E-430.83333434
ACTAGGC303.5786876E-430.83333427
CGAGACT303.5786876E-430.83333423
CCAAGCG250.005475178429.5999989
GCCAAGC250.005475178429.5999988
TTGACTG453.965406E-628.77777732
ATTGACT453.965406E-628.77777731
TATTGAC556.176251E-726.9090930
ATCTCCG358.818651E-426.4285710
TCCGAGC358.818651E-426.4285713
GCAGCGT358.818651E-426.428571
CTAGGCA358.818651E-426.4285728
GACTAGG358.818651E-426.4285726
CAGATGT451.3126897E-424.6666668
CTCCGAG400.001920242823.12500212
Produced by FastQC (version 0.11.5)