FastQCFastQC Report
Fri 10 Feb 2017
ERR1631300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29750
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC710.23865546218487396No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA680.2285714285714286No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC570.1915966386554622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT500.16806722689075632No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG410.13781512605042018No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC410.13781512605042018No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC390.13109243697478992No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG380.12773109243697478No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC370.12436974789915967No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC360.12100840336134452No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT360.12100840336134452No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC350.1176470588235294No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT350.1176470588235294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT340.1142857142857143No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT300.10084033613445378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAT251.2049594E-437.026
CGCCTTA251.2049594E-437.025
CTCTATG403.623063E-837.01
TCTATGG403.623063E-837.02
AGTCGGT355.389629E-737.011
CAGTCGG401.5318674E-632.37510
TCGGTGA401.5318674E-632.37513
TTATTGA401.5318674E-632.37529
GCAGTCG401.5318674E-632.3759
CGGTGAT401.5318674E-632.37514
CCTTATT303.5166155E-430.83333227
CTCGCCT303.5166155E-430.83333223
GATATGC250.005411774429.634
TATGGGC502.573379E-729.64
GGTGATT453.8385824E-628.77777915
TGATTCC453.8385824E-628.77777917
CTTATTG453.8385824E-628.77777928
GTGATTC453.8385824E-628.77777916
GATTCCT453.8385824E-628.77777918
ATTCCTC453.8385824E-628.77777919