##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631300.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29750
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.99112605042017	34.0	33.0	34.0	31.0	34.0
2	33.15075630252101	34.0	33.0	34.0	31.0	34.0
3	33.233512605042016	34.0	33.0	34.0	31.0	34.0
4	36.4706218487395	37.0	37.0	37.0	35.0	37.0
5	36.46783193277311	37.0	37.0	37.0	35.0	37.0
6	36.516806722689076	37.0	37.0	37.0	35.0	37.0
7	36.50467226890756	37.0	37.0	37.0	35.0	37.0
8	36.507932773109246	37.0	37.0	37.0	35.0	37.0
9	38.35673949579832	39.0	39.0	39.0	37.0	39.0
10	38.30904201680672	39.0	39.0	39.0	37.0	39.0
11	38.37613445378151	39.0	39.0	39.0	37.0	39.0
12	38.33744537815126	39.0	39.0	39.0	37.0	39.0
13	38.35532773109244	39.0	39.0	39.0	37.0	39.0
14	39.91250420168067	41.0	40.0	41.0	38.0	41.0
15	39.889512605042015	41.0	40.0	41.0	38.0	41.0
16	39.86860504201681	41.0	40.0	41.0	38.0	41.0
17	39.83690756302521	41.0	40.0	41.0	38.0	41.0
18	39.81388235294118	41.0	40.0	41.0	38.0	41.0
19	39.83401680672269	41.0	40.0	41.0	38.0	41.0
20	39.81179831932773	41.0	40.0	41.0	38.0	41.0
21	39.79852100840336	41.0	40.0	41.0	38.0	41.0
22	39.751697478991595	41.0	40.0	41.0	38.0	41.0
23	39.708302521008406	41.0	40.0	41.0	37.0	41.0
24	39.68564705882353	41.0	40.0	41.0	37.0	41.0
25	39.65690756302521	41.0	40.0	41.0	37.0	41.0
26	39.58373109243698	41.0	40.0	41.0	37.0	41.0
27	39.53838655462185	41.0	40.0	41.0	37.0	41.0
28	39.434621848739496	41.0	39.0	41.0	37.0	41.0
29	39.39193277310925	41.0	39.0	41.0	36.0	41.0
30	39.332470588235296	41.0	39.0	41.0	36.0	41.0
31	39.29361344537815	41.0	39.0	41.0	36.0	41.0
32	39.23068907563025	41.0	39.0	41.0	35.0	41.0
33	39.158151260504205	41.0	39.0	41.0	35.0	41.0
34	39.12379831932773	41.0	39.0	41.0	35.0	41.0
35	39.00857142857143	41.0	39.0	41.0	35.0	41.0
36	38.93872268907563	40.0	39.0	41.0	35.0	41.0
37	38.92494117647059	40.0	39.0	41.0	35.0	41.0
38	38.85152941176471	40.0	38.0	41.0	35.0	41.0
39	38.76141176470588	40.0	38.0	41.0	35.0	41.0
40	38.66968067226891	40.0	38.0	41.0	35.0	41.0
41	38.578823529411764	40.0	38.0	41.0	35.0	41.0
42	38.48275630252101	40.0	38.0	41.0	35.0	41.0
43	37.6453781512605	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	2.0
22	6.0
23	10.0
24	14.0
25	31.0
26	43.0
27	47.0
28	70.0
29	90.0
30	137.0
31	221.0
32	271.0
33	362.0
34	515.0
35	731.0
36	1254.0
37	2413.0
38	6064.0
39	17467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58823529411765	17.42857142857143	12.510924369747901	27.472268907563024
2	20.43361344537815	18.635294117647057	32.568067226890754	28.363025210084036
3	19.46890756302521	20.655462184873947	28.80672268907563	31.06890756302521
4	14.534453781512605	15.357983193277311	33.09243697478992	37.015126050420164
5	16.564705882352943	34.9546218487395	32.60504201680672	15.875630252100839
6	36.50756302521008	33.93613445378151	14.887394957983194	14.66890756302521
7	32.07731092436975	28.24201680672269	19.74453781512605	19.936134453781513
8	29.700840336134455	30.850420168067227	18.242016806722688	21.20672268907563
9	27.425210084033612	13.552941176470586	18.094117647058823	40.92773109243698
10	18.83361344537815	24.346218487394957	30.218487394957982	26.601680672268905
11	39.626890756302515	20.705882352941178	18.870588235294118	20.796638655462186
12	24.17142857142857	24.31260504201681	25.7109243697479	25.805042016806723
13	32.06386554621849	17.152941176470588	23.643697478991594	27.13949579831933
14	23.761344537815127	18.836974789915967	22.070588235294117	35.33109243697479
15	28.121008403361348	25.149579831932773	20.036974789915966	26.692436974789917
16	27.5563025210084	23.599999999999998	21.41512605042017	27.42857142857143
17	27.055462184873953	23.922689075630252	22.181512605042016	26.84033613445378
18	26.699159663865547	21.946218487394958	24.265546218487394	27.0890756302521
19	26.326050420168066	23.98655462184874	22.968067226890756	26.719327731092434
20	26.99495798319328	21.905882352941177	23.277310924369747	27.821848739495795
21	28.947899159663866	22.72941176470588	22.843697478991594	25.478991596638657
22	29.250420168067226	21.8218487394958	22.20840336134454	26.719327731092434
23	26.5109243697479	21.912605042016807	22.8	28.77647058823529
24	26.463865546218486	22.968067226890756	22.927731092436975	27.64033613445378
25	26.638655462184875	22.154621848739495	22.934453781512605	28.272268907563024
26	26.873949579831937	23.210084033613445	23.297478991596638	26.618487394957985
27	27.448739495798318	22.840336134453782	22.524369747899158	27.186554621848742
28	26.302521008403364	23.058823529411764	23.552941176470586	27.085714285714285
29	25.270588235294117	23.183193277310924	24.420168067226893	27.12605042016807
30	24.95798319327731	24.221848739495798	24.349579831932775	26.47058823529412
31	26.61176470588235	23.492436974789914	22.564705882352943	27.331092436974792
32	25.63361344537815	22.29243697478992	24.205042016806722	27.868907563025207
33	24.857142857142858	22.43697478991597	24.547899159663867	28.157983193277307
34	26.84033613445378	21.670588235294115	24.75966386554622	26.729411764705883
35	26.302521008403364	21.569747899159665	25.270588235294117	26.857142857142858
36	24.715966386554623	22.201680672268907	25.384873949579834	27.69747899159664
37	25.2672268907563	20.739495798319325	26.05378151260504	27.93949579831933
38	24.16806722689076	20.252100840336134	27.502521008403363	28.077310924369748
39	24.8	19.741176470588233	28.090756302521008	27.36806722689076
40	23.10924369747899	20.127731092436974	28.87058823529412	27.892436974789913
41	22.023529411764706	20.541176470588233	30.450420168067225	26.98487394957983
42	22.49411764705882	20.63529411764706	29.721008403361342	27.149579831932773
43	20.278991596638654	20.292436974789915	30.709243697478993	28.719327731092438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	5.0
6	4.0
7	3.0
8	4.5
9	6.0
10	9.0
11	12.0
12	12.0
13	23.5
14	35.0
15	57.0
16	79.0
17	68.5
18	58.0
19	58.0
20	72.0
21	86.0
22	75.5
23	65.0
24	73.5
25	82.0
26	82.0
27	92.0
28	102.0
29	146.5
30	191.0
31	241.0
32	291.0
33	291.0
34	444.5
35	598.0
36	720.5
37	843.0
38	997.5
39	1152.0
40	1152.0
41	1308.5
42	1465.0
43	1618.0
44	1771.0
45	1936.0
46	2101.0
47	2101.0
48	2259.0
49	2417.0
50	2452.5
51	2488.0
52	2525.0
53	2562.0
54	2562.0
55	2388.0
56	2214.0
57	2149.5
58	2085.0
59	2060.5
60	2036.0
61	2036.0
62	1935.0
63	1834.0
64	1741.0
65	1648.0
66	1456.0
67	1264.0
68	1264.0
69	1066.0
70	868.0
71	740.5
72	613.0
73	439.0
74	265.0
75	265.0
76	220.0
77	175.0
78	156.5
79	138.0
80	104.5
81	71.0
82	71.0
83	59.0
84	47.0
85	43.0
86	39.0
87	32.5
88	26.0
89	26.0
90	18.5
91	11.0
92	7.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	29750.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.13781512605043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.95187850426029	68.4873949579832
2	5.077038541344753	7.73109243697479
3	1.7306079201801245	3.9529411764705884
4	0.9403558341795064	2.8638655462184874
5	0.5518520153635601	2.100840336134454
6	0.4458964284137566	2.0369747899159663
7	0.2781334157432343	1.4823529411764707
8	0.18983709328506468	1.1563025210084033
9	0.15010374817888836	1.0285714285714285
>10	0.6710520506820891	8.500840336134454
>50	0.013244448368725443	0.6588235294117647
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	71	0.23865546218487396	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	68	0.2285714285714286	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	57	0.1915966386554622	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.16806722689075632	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	41	0.13781512605042018	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	41	0.13781512605042018	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	39	0.13109243697478992	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	38	0.12773109243697478	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	37	0.12436974789915967	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	36	0.12100840336134452	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	36	0.12100840336134452	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	35	0.1176470588235294	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	35	0.1176470588235294	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34	0.1142857142857143	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	30	0.10084033613445378	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0033613445378151263	0.0
17	0.0	0.0	0.0	0.0033613445378151263	0.0
18	0.0	0.0	0.0	0.0033613445378151263	0.0
19	0.0	0.0	0.0	0.0033613445378151263	0.0
20	0.0	0.0	0.0	0.0033613445378151263	0.0
21	0.0	0.0	0.0	0.0033613445378151263	0.0
22	0.0	0.0	0.0	0.0067226890756302525	0.0
23	0.0	0.0	0.0	0.013445378151260505	0.0
24	0.0	0.0	0.0	0.01680672268907563	0.0
25	0.0	0.0	0.0	0.023529411764705882	0.0
26	0.0	0.0	0.0	0.040336134453781515	0.0
27	0.0	0.0	0.0	0.08403361344537816	0.0
28	0.0	0.0	0.0	0.35294117647058826	0.0
29	0.0	0.0	0.0	0.7294117647058823	0.0
30	0.0	0.0	0.0	1.1966386554621848	0.0
31	0.0	0.0	0.0	2.3260504201680674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTAT	25	1.2049594E-4	37.0	26
CGCCTTA	25	1.2049594E-4	37.0	25
CTCTATG	40	3.623063E-8	37.0	1
TCTATGG	40	3.623063E-8	37.0	2
AGTCGGT	35	5.389629E-7	37.0	11
CAGTCGG	40	1.5318674E-6	32.375	10
TCGGTGA	40	1.5318674E-6	32.375	13
TTATTGA	40	1.5318674E-6	32.375	29
GCAGTCG	40	1.5318674E-6	32.375	9
CGGTGAT	40	1.5318674E-6	32.375	14
CCTTATT	30	3.5166155E-4	30.833332	27
CTCGCCT	30	3.5166155E-4	30.833332	23
GATATGC	25	0.0054117744	29.6	34
TATGGGC	50	2.573379E-7	29.6	4
GGTGATT	45	3.8385824E-6	28.777779	15
TGATTCC	45	3.8385824E-6	28.777779	17
CTTATTG	45	3.8385824E-6	28.777779	28
GTGATTC	45	3.8385824E-6	28.777779	16
GATTCCT	45	3.8385824E-6	28.777779	18
ATTCCTC	45	3.8385824E-6	28.777779	19
>>END_MODULE