FastQCFastQC Report
Fri 10 Feb 2017
ERR1631299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111601
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.24469211524638787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21660.19485408883223387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19910.17911102994689643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.024.1144281
GTATAAA903.8289727E-922.611111
CGTATAT701.2191514E-418.510
TAGACAG1501.2732926E-1118.55
CCGTTTA500.00703473918.527
TACGCTA953.606172E-617.5263169
CGTTATT1603.45608E-1117.343752
TATACAC6650.017.2481237
TTAGCGA650.001579889417.07692327
ATACACA4850.016.78350437
ACGCTAT1005.8774385E-616.65000210
GCGTATA803.3824402E-416.18759
CTTATAC18600.015.81451637
TTTATAC957.059343E-515.5789483
GTTATTC1902.7284841E-1115.5789483
TAATACT2055.456968E-1215.3414644
GCGTTAT2055.456968E-1215.3414641
CCGCTAT1101.4517036E-515.13636425
GCTTATA750.00410429314.81
GCATAGA750.00410429314.81
Produced by FastQC (version 0.11.5)