##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631299.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1111601
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02435945991412	34.0	33.0	34.0	31.0	34.0
2	33.175218446187074	34.0	33.0	34.0	31.0	34.0
3	33.26819065474032	34.0	34.0	34.0	31.0	34.0
4	36.498012326365306	37.0	37.0	37.0	35.0	37.0
5	36.47921961207304	37.0	37.0	37.0	35.0	37.0
6	36.53009847958035	37.0	37.0	37.0	35.0	37.0
7	36.538361336486744	37.0	37.0	37.0	35.0	37.0
8	36.53324799096079	37.0	37.0	37.0	35.0	37.0
9	38.39058798975532	39.0	39.0	39.0	37.0	39.0
10	38.34811321688267	39.0	39.0	39.0	37.0	39.0
11	38.40597210689807	39.0	39.0	39.0	37.0	39.0
12	38.35963173836655	39.0	39.0	39.0	37.0	39.0
13	38.39198327457424	39.0	39.0	39.0	37.0	39.0
14	39.93793276544372	41.0	40.0	41.0	38.0	41.0
15	39.92466451541515	41.0	40.0	41.0	38.0	41.0
16	39.89211326726046	41.0	40.0	41.0	38.0	41.0
17	39.88512604792547	41.0	40.0	41.0	38.0	41.0
18	39.860649639573914	41.0	40.0	41.0	38.0	41.0
19	39.879485534827694	41.0	40.0	41.0	38.0	41.0
20	39.84171568755336	41.0	40.0	41.0	38.0	41.0
21	39.81722758435806	41.0	40.0	41.0	38.0	41.0
22	39.76450093153928	41.0	40.0	41.0	38.0	41.0
23	39.71236171971778	41.0	40.0	41.0	37.0	41.0
24	39.68758214503225	41.0	40.0	41.0	37.0	41.0
25	39.67655300777887	41.0	40.0	41.0	37.0	41.0
26	39.57517850379767	41.0	40.0	41.0	37.0	41.0
27	39.53077408170738	41.0	40.0	41.0	37.0	41.0
28	39.48126351091803	41.0	40.0	41.0	37.0	41.0
29	39.43053667637938	41.0	39.0	41.0	36.0	41.0
30	39.36600632781007	41.0	39.0	41.0	36.0	41.0
31	39.30890850224136	41.0	39.0	41.0	36.0	41.0
32	39.23505556400183	41.0	39.0	41.0	35.0	41.0
33	39.168119676034834	41.0	39.0	41.0	35.0	41.0
34	39.115354340271374	41.0	39.0	41.0	35.0	41.0
35	39.02980745789181	41.0	39.0	41.0	35.0	41.0
36	38.962883264768564	41.0	39.0	41.0	35.0	41.0
37	38.90660857627872	41.0	39.0	41.0	35.0	41.0
38	38.83395840773803	40.0	38.0	41.0	35.0	41.0
39	38.753533866918076	40.0	38.0	41.0	35.0	41.0
40	38.66393966899994	40.0	38.0	41.0	35.0	41.0
41	38.5866601415436	40.0	38.0	41.0	35.0	41.0
42	38.4645650732592	40.0	38.0	41.0	35.0	41.0
43	37.68043839471177	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	1.0
13	2.0
14	2.0
15	5.0
16	2.0
17	6.0
18	13.0
19	40.0
20	56.0
21	128.0
22	223.0
23	364.0
24	668.0
25	983.0
26	1402.0
27	2090.0
28	2899.0
29	4043.0
30	5478.0
31	7308.0
32	9353.0
33	12606.0
34	19028.0
35	28505.0
36	45854.0
37	89414.0
38	217031.0
39	664093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.435909107674426	17.65975381454317	12.099755217924416	26.80458185985799
2	19.598489026188354	19.354606553970356	33.196263767305	27.850640652536296
3	19.91577913298027	21.413438814826545	28.514008173796174	30.156773878397015
4	14.825733334172963	15.341116101910668	33.54198134042701	36.29116922348936
5	16.269326853790165	34.620155973231405	32.65695155006158	16.453565622916855
6	35.077964125617015	33.85684251813375	15.625300804875131	15.439892551374099
7	31.18376107973994	28.79981216281741	20.478481037710473	19.537945719732168
8	28.769225648411616	31.446985024302787	19.211929460300954	20.571859866984646
9	27.506902206817013	13.99854804016909	18.8701701419844	39.6243796110295
10	18.909212928020036	24.794777982387565	31.082465740854857	25.213543348737545
11	37.62465129124569	20.87700532835073	20.31952112313681	21.178822257266773
12	23.389687486787075	24.388247221799908	27.183944598826375	25.03812069258664
13	31.602436485753433	18.27931065193356	23.473710441066533	26.64454242124647
14	23.925941052589913	19.219216247556453	23.735495020245573	33.11934767960806
15	26.882487511256286	25.65551848190133	20.882942710558915	26.579051296283467
16	26.721818350289357	24.180528804849942	22.76167437776684	26.335978467093856
17	25.29189880181828	24.92674979601494	23.882760091075845	25.89859131109094
18	25.529304129809166	23.648863216207975	24.7217301891596	26.10010246482326
19	26.141394259271088	23.80611388438837	24.885997763586033	25.166494092754505
20	26.09731369439214	23.74665010197004	24.433047469370756	25.722988734267062
21	26.84632345598825	23.75357704787959	23.924231806196648	25.475867689935505
22	27.31618629346321	23.219752411161917	23.75177784114984	25.712283454225034
23	26.113326634286942	23.542889939825532	24.176840431053947	26.166942994833576
24	25.637346493930824	23.828514008173794	23.91307672447218	26.621062773423198
25	26.25456436257254	23.11018072131997	24.19591202238933	26.43934289371816
26	25.755194534729636	23.926480814608837	24.33400113889786	25.984323511763662
27	25.855860151259307	23.575635502307033	24.124033713535702	26.444470632897954
28	25.57374453603406	23.73495525822665	24.86485708451144	25.82644312122785
29	25.35774976812723	23.769230146428438	25.135097935320317	25.73792215012401
30	25.106490548317247	24.383389363629576	24.640226124301794	25.869893963751384
31	25.871693170481137	23.86035996729042	24.292979225459497	25.974967636768948
32	24.997278699821248	23.623674321991434	24.74161142352337	26.637435554663945
33	24.70382808219856	23.44789182449458	25.30089483546704	26.547385257839817
34	25.868634519040555	22.622325816547484	25.21552247614027	26.293517188271693
35	25.24179089439466	23.28875198924794	25.749257152521455	25.720199963835945
36	24.76985896918049	23.19249442920616	25.334180159967467	26.70346644164588
37	24.69726097763496	22.112700510344986	26.262390911846968	26.92764760017308
38	23.978567849435183	22.37745378062812	27.14337248707045	26.500605882866246
39	23.868366437237825	22.023100015203298	27.68646303844635	26.422070509112533
40	23.658309051539174	22.001779415455726	28.30601987583674	26.033891657168358
41	22.914606949795836	21.972362385424265	28.816994587086555	26.296036077693348
42	22.559983303361548	22.14004845263723	28.93934064470975	26.360627599291476
43	22.046039901007646	21.492783831608644	29.339574181743274	27.12160208564044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	150.5
2	141.0
3	322.0
4	503.0
5	503.0
6	613.5
7	724.0
8	646.0
9	568.0
10	886.5
11	1205.0
12	1205.0
13	2044.5
14	2884.0
15	4103.0
16	5322.0
17	5018.0
18	4714.0
19	4714.0
20	5265.5
21	5817.0
22	5244.5
23	4672.0
24	5447.5
25	6223.0
26	6223.0
27	7576.0
28	8929.0
29	11716.0
30	14503.0
31	17295.5
32	20088.0
33	20088.0
34	25138.5
35	30189.0
36	35616.0
37	41043.0
38	45745.0
39	50447.0
40	50447.0
41	55302.0
42	60157.0
43	64281.5
44	68406.0
45	71800.5
46	75195.0
47	75195.0
48	78090.5
49	80986.0
50	81910.0
51	82834.0
52	82852.5
53	82871.0
54	82871.0
55	80926.5
56	78982.0
57	75319.0
58	71656.0
59	70226.5
60	68797.0
61	68797.0
62	64378.0
63	59959.0
64	55133.0
65	50307.0
66	45292.5
67	40278.0
68	40278.0
69	35285.0
70	30292.0
71	26551.5
72	22811.0
73	18697.5
74	14584.0
75	14584.0
76	11939.5
77	9295.0
78	7754.5
79	6214.0
80	5231.5
81	4249.0
82	4249.0
83	3441.5
84	2634.0
85	2112.5
86	1591.0
87	1237.0
88	883.0
89	883.0
90	623.5
91	364.0
92	222.5
93	81.0
94	53.0
95	25.0
96	25.0
97	17.0
98	9.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1111601.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.56866370563603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.35492024784939	53.78325707772495
2	7.034277360189481	8.661821144033507
3	1.9721631796848247	3.6427035474795852
4	0.9001857322391645	2.2169293048337937
5	0.5212037203919345	1.6044908291468687
6	0.3575623201738121	1.3208780546753038
7	0.2586933609610629	1.1149183180724676
8	0.1800371150137102	0.8867715671049631
9	0.15107709982983353	0.8371453637741241
>10	1.0749807121424855	12.989076746936142
>50	0.12790488904945552	5.487204507467916
>100	0.06611660884366702	6.6523695138298615
>500	4.3882681563835434E-4	0.18309184878376236
>1k	4.3882681563835434E-4	0.619342176136711
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2720	0.24469211524638787	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2166	0.19485408883223387	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1991	0.17911102994689643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.799206729752852E-4	0.0
5	0.0	8.99603364876426E-5	0.0	1.799206729752852E-4	0.0
6	0.0	8.99603364876426E-5	0.0	2.698810094629278E-4	0.0
7	0.0	8.99603364876426E-5	0.0	2.698810094629278E-4	0.0
8	0.0	8.99603364876426E-5	0.0	2.698810094629278E-4	0.0
9	0.0	8.99603364876426E-5	0.0	2.698810094629278E-4	0.0
10	0.0	8.99603364876426E-5	0.0	3.598413459505704E-4	0.0
11	0.0	8.99603364876426E-5	0.0	5.397620189258556E-4	0.0
12	0.0	8.99603364876426E-5	0.0	6.297223554134982E-4	0.0
13	0.0	8.99603364876426E-5	0.0	6.297223554134982E-4	0.0
14	0.0	1.799206729752852E-4	0.0	9.895637013640685E-4	0.0
15	0.0	1.799206729752852E-4	0.0	0.0016192860567775668	8.99603364876426E-5
16	0.0	1.799206729752852E-4	0.0	0.001979127402728137	8.99603364876426E-5
17	0.0	1.799206729752852E-4	0.0	0.002249008412191065	8.99603364876426E-5
18	0.0	2.698810094629278E-4	0.0	0.002249008412191065	8.99603364876426E-5
19	0.0	2.698810094629278E-4	0.0	0.002878730767604563	8.99603364876426E-5
20	0.0	2.698810094629278E-4	0.0	0.003328532450042776	8.99603364876426E-5
21	0.0	2.698810094629278E-4	0.0	0.0042281358149192025	8.99603364876426E-5
22	0.0	2.698810094629278E-4	0.0	0.005757461535209126	8.99603364876426E-5
23	0.0	2.698810094629278E-4	0.0	0.008186390620375477	8.99603364876426E-5
24	0.0	3.598413459505704E-4	0.0	0.012594447108269964	8.99603364876426E-5
25	0.0	3.598413459505704E-4	0.0	0.016372781240750953	8.99603364876426E-5
26	0.0	3.598413459505704E-4	0.0	0.02347964782327472	8.99603364876426E-5
27	0.0	3.598413459505704E-4	0.0	0.06639072832788023	8.99603364876426E-5
28	0.0	3.598413459505704E-4	0.0	0.22463096020964357	8.99603364876426E-5
29	0.0	3.598413459505704E-4	0.0	0.44440406224895446	8.99603364876426E-5
30	0.0	3.598413459505704E-4	0.0	0.7242706690620105	8.99603364876426E-5
31	0.0	3.598413459505704E-4	0.0	1.354172945148484	8.99603364876426E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1005	0.0	24.114428	1
GTATAAA	90	3.8289727E-9	22.61111	1
CGTATAT	70	1.2191514E-4	18.5	10
TAGACAG	150	1.2732926E-11	18.5	5
CCGTTTA	50	0.007034739	18.5	27
TACGCTA	95	3.606172E-6	17.526316	9
CGTTATT	160	3.45608E-11	17.34375	2
TATACAC	665	0.0	17.24812	37
TTAGCGA	65	0.0015798894	17.076923	27
ATACACA	485	0.0	16.783504	37
ACGCTAT	100	5.8774385E-6	16.650002	10
GCGTATA	80	3.3824402E-4	16.1875	9
CTTATAC	1860	0.0	15.814516	37
TTTATAC	95	7.059343E-5	15.578948	3
GTTATTC	190	2.7284841E-11	15.578948	3
TAATACT	205	5.456968E-12	15.341464	4
GCGTTAT	205	5.456968E-12	15.341464	1
CCGCTAT	110	1.4517036E-5	15.136364	25
GCTTATA	75	0.004104293	14.8	1
GCATAGA	75	0.004104293	14.8	1
>>END_MODULE