FastQCFastQC Report
Fri 10 Feb 2017
ERR1631298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438945
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.43627333720625594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14240.3244142204604221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12940.2947977537049061No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6080.1385139368257982No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.13623574707537392No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5610.12780644499880395No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5480.12484479832325233No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT5060.11527640137147023No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC4730.10775837519507No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4570.10411327159439109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTC250.005492689629.59999829
CCGCTCA405.9318925E-527.7500029
TATAACA451.3213922E-424.6666662
ATACACA1850.023.037
GGCCTAA502.698417E-422.19999910
AGCGTCA450.003822096920.5555553
GTATTTC450.003822096920.5555551
CCAATAC450.003822096920.5555553
GTATAAA450.003822096920.5555551
TCTGGAC450.003822096920.5555553
GGTATCA8350.019.9401191
ACTCTAA1355.4569682E-1119.18518410
CAGTCGG1205.14774E-918.510
ATACCAT609.2244166E-418.56
GATGTGT500.007028126618.4999986
GGTTTAC500.007028126618.49999826
GCCTAAA500.007028126618.49999811
GATTTAG953.596122E-617.5263161
CTTCGGG1401.8590072E-917.17857234
TGAACAC1401.8590072E-917.1785725