FastQCFastQC Report
Fri 10 Feb 2017
ERR1631297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486134
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11080.22792069676262103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11070.22771499216265473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7780.16003817877375373No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6150.12650832897925263No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5480.11272612078151291No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT4990.10264659538316595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT250.005493261429.66
CGAACTA451.3216781E-424.66666624
GACCGTG400.001929592823.1250027
ACCGTGA400.001929592823.1250028
GGTATCA5900.021.9491521
CACTCTA1107.4578566E-1121.8636369
CTGCGCA555.137227E-420.1818189
GAACACT1202.382876E-1020.0416666
ATACACA1850.020.037
TATACAC2950.019.44067837
ACTCTAA1153.0377123E-919.30434810
TTAATAC500.00702918418.53
CCATACC500.00702918418.54
TAGTACT500.00702918418.54
GCATCAG2350.017.31914715
TCTACCC752.0641752E-417.2666663
TAACAGA752.0641752E-417.26666610
TGAACAC1553.9835868E-1016.7096775
GTCCTAT1005.8637634E-616.6500021
TAATACA904.4374705E-516.4444454
Produced by FastQC (version 0.11.5)