FastQCFastQC Report
Fri 10 Feb 2017
ERR1631296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672724
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA70200.191138784183075No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC57830.15745806110124258No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC50590.1377451722481733No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC49480.13472289232732979No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT41760.11370307161659846No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC41510.11302237794073283No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC41410.11275010047038656No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT36950.10060652529294332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.023.2628731
TCTAGAC6600.019.9015163
CTTATAC50250.019.2179137
CTAGACA6900.018.54
ATACACA10300.018.537
CAAGACG13700.017.6897814
ACTGATC5800.017.5431048
GTCTAGA6600.017.3787881
GTTCTAG7500.017.2666661
AAGACGG13800.017.159425
ACGGACC13650.017.0769238
GTATCAA24850.016.8993952
TATACAC15550.016.77491837
AGACGGA14550.016.529216
CTCTAGT7050.016.00709227
TAGAACT8850.015.8870054
GACGGAC15300.015.5980397
CGCAAGA15150.015.5082512
GCGAAAG14700.015.47959218
GAGCGAA15200.015.33552616
Produced by FastQC (version 0.11.5)