##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631296.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3672724
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8587751216808	34.0	33.0	34.0	31.0	34.0
2	33.035586665374254	34.0	33.0	34.0	31.0	34.0
3	33.15087357503586	34.0	33.0	34.0	31.0	34.0
4	36.479748001755645	37.0	37.0	37.0	35.0	37.0
5	36.45319795334471	37.0	37.0	37.0	35.0	37.0
6	36.495436629596995	37.0	37.0	37.0	35.0	37.0
7	36.47919636760072	37.0	37.0	37.0	35.0	37.0
8	36.456503674112184	37.0	37.0	37.0	35.0	37.0
9	38.316591990032464	39.0	39.0	39.0	37.0	39.0
10	38.27661784550105	39.0	39.0	39.0	37.0	39.0
11	38.33849099469494	39.0	39.0	39.0	37.0	39.0
12	38.113298195018196	39.0	39.0	39.0	37.0	39.0
13	38.238838529658096	39.0	39.0	39.0	37.0	39.0
14	39.77758089091367	41.0	40.0	41.0	38.0	41.0
15	39.78510909069127	41.0	40.0	41.0	38.0	41.0
16	39.77886386235394	41.0	40.0	41.0	38.0	41.0
17	39.76624516299074	41.0	40.0	41.0	38.0	41.0
18	39.74380323705239	41.0	40.0	41.0	38.0	41.0
19	39.74742806701511	41.0	40.0	41.0	38.0	41.0
20	39.7220191334824	41.0	40.0	41.0	38.0	41.0
21	39.68455293673034	41.0	40.0	41.0	37.0	41.0
22	39.640217179401446	41.0	40.0	41.0	37.0	41.0
23	39.577087469681906	41.0	40.0	41.0	37.0	41.0
24	39.53942877275831	41.0	39.0	41.0	37.0	41.0
25	39.51860608093611	41.0	39.0	41.0	37.0	41.0
26	39.46296291254121	41.0	39.0	41.0	37.0	41.0
27	39.39328139005272	41.0	39.0	41.0	37.0	41.0
28	39.29870499389554	41.0	39.0	41.0	36.0	41.0
29	39.2323174842433	41.0	39.0	41.0	36.0	41.0
30	39.1835607576284	41.0	39.0	41.0	36.0	41.0
31	39.15097567908724	41.0	39.0	41.0	35.0	41.0
32	39.07416266509544	40.0	39.0	41.0	35.0	41.0
33	38.978378173802334	40.0	39.0	41.0	35.0	41.0
34	38.93246892497231	40.0	39.0	41.0	35.0	41.0
35	38.83499549653064	40.0	38.0	41.0	35.0	41.0
36	38.785820823998755	40.0	38.0	41.0	35.0	41.0
37	38.72677146444982	40.0	38.0	41.0	35.0	41.0
38	38.64102203160379	40.0	38.0	41.0	35.0	41.0
39	38.57078587990821	40.0	38.0	41.0	35.0	41.0
40	38.477789782188914	40.0	38.0	41.0	35.0	41.0
41	38.39724438863362	40.0	37.0	41.0	34.0	41.0
42	38.28775344948327	40.0	37.0	41.0	34.0	41.0
43	37.326679870308794	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	1.0
14	4.0
15	5.0
16	9.0
17	23.0
18	55.0
19	111.0
20	254.0
21	483.0
22	876.0
23	1471.0
24	2249.0
25	3508.0
26	5123.0
27	7121.0
28	10237.0
29	14127.0
30	19310.0
31	25895.0
32	34369.0
33	47501.0
34	71994.0
35	108389.0
36	181375.0
37	338073.0
38	805272.0
39	1994886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77278989654545	16.518338976737702	11.348715558261388	27.360155568455458
2	21.014402389071435	19.01161100044545	31.623585110125347	28.350401500357776
3	20.710213999200594	19.464326750390175	27.839608965988187	31.985850284421048
4	15.349451796541205	14.392723221238516	31.961835411536505	38.29598957068377
5	17.495787867533743	32.846410457197436	31.725580250517055	17.932221424751766
6	37.37098132067642	32.330689700614585	14.968072743827198	15.330256234881793
7	32.834729753719586	27.905472886064945	18.897445057129257	20.36235230308621
8	29.160073013926446	30.891703269834597	18.555519009868423	21.39270470637053
9	27.76116582678143	13.974341660304448	17.774382175192038	40.490110337722086
10	19.076331355146753	23.60591212408011	29.474580719923416	27.843175800849725
11	39.520394127083875	19.873750382549847	19.287645899882484	21.318209590483793
12	25.576166354999724	22.84906243975861	25.407898878325735	26.166872326915936
13	32.236999023068435	16.700329237917142	23.187149374687564	27.87552236432686
14	25.15454469216854	18.456518921650524	21.559202379487267	34.82973400669367
15	27.993609103216034	24.622487287364912	19.577648633548286	27.80625497587077
16	28.413569873478107	23.205065232236347	21.011243970415418	27.37012092387013
17	27.22121237533776	23.453463968433237	21.834148169042923	27.491175487186077
18	27.774099006622876	21.508095898303274	23.185161749154034	27.532643345919812
19	27.43829920244483	22.706606867273447	22.21127969321953	27.64381423706219
20	27.951242728830156	21.73601936873013	22.279403516300164	28.03333438613955
21	29.594192212646526	22.12270783211589	22.065366196860968	26.217733758376617
22	29.234922090524634	21.828675391888964	21.307100669693664	27.629301847892734
23	27.554017127341996	21.751511956792832	22.51751016411797	28.176960751747202
24	27.089974634630863	22.64994592569439	22.185549472271806	28.07452996740294
25	27.770314349785064	21.68992279300051	22.19091333843763	28.3488495187768
26	27.826839152628946	22.711725683715954	22.281009953375204	27.18042521027989
27	28.086101759892657	21.570420211265535	22.38172538965629	27.961752639185523
28	27.06854639771461	22.00453940998561	22.97896602086081	27.947948171438963
29	26.522493930935187	22.494475490126675	22.984738303232152	27.99829227570599
30	25.83199826613707	23.187339968916802	23.607409650167018	27.373252114779113
31	27.925430824641328	22.484455679217934	21.78091792359023	27.80919557255051
32	26.637939578362	21.901319020977343	22.44342346443675	29.0173179362239
33	26.310008593076965	21.70699459039122	23.250290520060858	28.732706296470955
34	27.77989851674125	21.27992737815311	23.317651966224524	27.622522138881113
35	27.09571968925517	21.23374911918238	24.332947425398697	27.337583766163753
36	26.171991143358447	21.684994570787243	24.219571086746512	27.9234431991078
37	26.69544458009913	20.64056542228602	24.74849730064116	27.915492696973693
38	25.452525155715485	20.084847105309304	25.96309986810879	28.49952787086642
39	25.96712957466992	19.73415372350332	26.147622309762454	28.15109439206431
40	25.128079322050883	20.735345209713554	26.578555862079483	27.558019606156087
41	24.469440121283277	20.27565915652796	28.010680900606744	27.24421982158202
42	25.0555173762036	20.308740869175033	27.902695655867415	26.73304609875395
43	22.936599646474935	19.645745228881886	28.13660923064189	29.281045894001288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	24.5
2	25.0
3	79.5
4	134.0
5	134.0
6	186.5
7	239.0
8	216.0
9	193.0
10	313.0
11	433.0
12	433.0
13	734.0
14	1035.0
15	1664.0
16	2293.0
17	2367.0
18	2441.0
19	2441.0
20	3065.5
21	3690.0
22	4236.5
23	4783.0
24	6121.5
25	7460.0
26	7460.0
27	9882.5
28	12305.0
29	16450.5
30	20596.0
31	26538.0
32	32480.0
33	32480.0
34	43856.0
35	55232.0
36	71934.0
37	88636.0
38	107196.0
39	125756.0
40	125756.0
41	138901.5
42	152047.0
43	169271.5
44	186496.0
45	209028.5
46	231561.0
47	231561.0
48	247630.0
49	263699.0
50	274687.0
51	285675.0
52	300685.5
53	315696.0
54	315696.0
55	309810.0
56	303924.0
57	301007.0
58	298090.0
59	293583.5
60	289077.0
61	289077.0
62	270837.0
63	252597.0
64	240637.5
65	228678.0
66	203943.0
67	179208.0
68	179208.0
69	151266.5
70	123325.0
71	105409.5
72	87494.0
73	66268.5
74	45043.0
75	45043.0
76	34975.5
77	24908.0
78	20553.5
79	16199.0
80	14470.0
81	12741.0
82	12741.0
83	10238.0
84	7735.0
85	6447.5
86	5160.0
87	4208.5
88	3257.0
89	3257.0
90	2536.0
91	1815.0
92	1081.5
93	348.0
94	240.5
95	133.0
96	133.0
97	93.0
98	53.0
99	31.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3672724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.73271443705299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.70563409781133	36.197016731112704
2	7.847385213654594	6.706801428253077
3	2.4411866539982343	3.129555965185543
4	1.1841218264522773	2.0240295947386713
5	0.7221439641192204	1.5429585900574045
6	0.47485763565050454	1.2175173445504297
7	0.36282214078100455	1.0853062453404436
8	0.2615950239342929	0.8942932364750544
9	0.20556119541211534	0.7905769076596655
>10	1.4343377363344698	11.920238355468557
>50	0.16666677002224298	5.018834462536506
>100	0.1617034241769576	14.203291006762205
>500	0.020126404294728926	6.073829715904755
>1k	0.01166562287121702	8.70650461149965
>5k	1.9229048688819262E-4	0.48924580445532984
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	7020	0.191138784183075	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	5783	0.15745806110124258	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	5059	0.1377451722481733	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	4948	0.13472289232732979	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4176	0.11370307161659846	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	4151	0.11302237794073283	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	4141	0.11275010047038656	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	3695	0.10060652529294332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.7227747034626072E-5	0.0	0.0	0.0
3	0.0	2.7227747034626072E-5	0.0	0.0	0.0
4	0.0	2.7227747034626072E-5	0.0	0.0	0.0
5	0.0	8.16832411038782E-5	0.0	0.0	0.0
6	0.0	8.16832411038782E-5	0.0	0.0	0.0
7	0.0	8.16832411038782E-5	0.0	0.0	0.0
8	0.0	1.0891098813850429E-4	0.0	0.0	0.0
9	0.0	1.0891098813850429E-4	0.0	2.7227747034626072E-5	0.0
10	0.0	1.0891098813850429E-4	0.0	2.7227747034626072E-5	2.7227747034626072E-5
11	0.0	1.3613873517313036E-4	0.0	8.16832411038782E-5	2.7227747034626072E-5
12	0.0	1.3613873517313036E-4	0.0	8.16832411038782E-5	2.7227747034626072E-5
13	0.0	1.3613873517313036E-4	0.0	8.16832411038782E-5	2.7227747034626072E-5
14	0.0	1.3613873517313036E-4	0.0	1.0891098813850429E-4	2.7227747034626072E-5
15	0.0	1.3613873517313036E-4	0.0	1.0891098813850429E-4	2.7227747034626072E-5
16	0.0	1.633664822077564E-4	0.0	1.905942292423825E-4	2.7227747034626072E-5
17	0.0	1.633664822077564E-4	0.0	2.4504972331163466E-4	2.7227747034626072E-5
18	0.0	2.1782197627700858E-4	0.0	2.4504972331163466E-4	2.7227747034626072E-5
19	0.0	2.1782197627700858E-4	0.0	4.0841620551939104E-4	2.7227747034626072E-5
20	0.0	2.1782197627700858E-4	0.0	5.717826877271474E-4	2.7227747034626072E-5
21	0.0	2.1782197627700858E-4	0.0	7.6237691696953E-4	5.4455494069252144E-5
22	0.0	2.1782197627700858E-4	0.0	0.001170793122488921	5.4455494069252144E-5
23	0.0	2.722774703462607E-4	0.0	0.002014853280562329	5.4455494069252144E-5
24	0.0	2.9950521738088677E-4	0.0	0.0032945573911897547	5.4455494069252144E-5
25	0.0	2.9950521738088677E-4	0.0	0.004683172489955684	5.4455494069252144E-5
26	0.0	2.9950521738088677E-4	0.0	0.00838614608666483	5.4455494069252144E-5
27	0.0	2.9950521738088677E-4	0.0	0.0337351785759017	5.4455494069252144E-5
28	0.0	3.267329644155128E-4	0.0	0.16246796655561377	5.4455494069252144E-5
29	0.0	3.5396071145013893E-4	0.0	0.35420576117345054	5.4455494069252144E-5
30	0.0	3.5396071145013893E-4	0.0	0.6020599424296517	5.4455494069252144E-5
31	0.0	3.5396071145013893E-4	0.0	1.2954689761604739	5.4455494069252144E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1845	0.0	23.262873	1
TCTAGAC	660	0.0	19.901516	3
CTTATAC	5025	0.0	19.21791	37
CTAGACA	690	0.0	18.5	4
ATACACA	1030	0.0	18.5	37
CAAGACG	1370	0.0	17.689781	4
ACTGATC	580	0.0	17.543104	8
GTCTAGA	660	0.0	17.378788	1
GTTCTAG	750	0.0	17.266666	1
AAGACGG	1380	0.0	17.15942	5
ACGGACC	1365	0.0	17.076923	8
GTATCAA	2485	0.0	16.899395	2
TATACAC	1555	0.0	16.774918	37
AGACGGA	1455	0.0	16.52921	6
CTCTAGT	705	0.0	16.007092	27
TAGAACT	885	0.0	15.887005	4
GACGGAC	1530	0.0	15.598039	7
CGCAAGA	1515	0.0	15.508251	2
GCGAAAG	1470	0.0	15.479592	18
GAGCGAA	1520	0.0	15.335526	16
>>END_MODULE