FastQCFastQC Report
Fri 10 Feb 2017
ERR1631295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26550
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4741.7853107344632766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4211.585687382297552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3301.2429378531073447No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.5649717514124294No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.21468926553672316No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA560.21092278719397364No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA540.2033898305084746No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.19962335216572505No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA380.1431261770244821No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA370.13935969868173256No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA350.1318267419962335No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.12429378531073447No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.11299435028248588No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC280.10546139359698682No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.10546139359698682No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA270.1016949152542373No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA270.1016949152542373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTA1351.9506842E-816.44444537
ACTGTCT750.003965734514.837
GGTATCA2850.014.2807021
GTCTCTT1754.68277E-712.68571536
CTCTATG1100.003582801211.7727271
TCTATGG1150.004994002611.2608692
AACGCAG3501.8189894E-1211.17
GTATCAA3700.011.02
TATGGGC1200.00685651410.7916674
ATCAACG3801.8189894E-1210.7105264
TGTCTCT2452.0791231E-710.57142837
TCAACGC3901.8189894E-1210.4358985
CAACGCA3901.8189894E-1210.4358986
CCTGTCT1604.4973142E-410.4062536
CTATGGG1250.00928376310.363
TATCAAC4053.6379788E-1210.0493823
ATGGGAA1550.00409813839.54838819
GAGTACA2708.369681E-68.9074086
CGCAGAG4402.0190782E-108.8295452
AGTACAT2751.0559639E-58.7454547
Produced by FastQC (version 0.11.5)