##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631295.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26550
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.424896421845574	33.0	31.0	34.0	31.0	34.0
2	32.55623352165725	34.0	31.0	34.0	31.0	34.0
3	32.622937853107345	34.0	31.0	34.0	31.0	34.0
4	36.14745762711864	37.0	35.0	37.0	35.0	37.0
5	36.05325800376648	37.0	35.0	37.0	35.0	37.0
6	36.110885122410544	37.0	36.0	37.0	35.0	37.0
7	36.12297551789077	37.0	36.0	37.0	35.0	37.0
8	36.14161958568738	37.0	36.0	37.0	35.0	37.0
9	37.87566854990584	39.0	38.0	39.0	35.0	39.0
10	37.83502824858757	39.0	38.0	39.0	35.0	39.0
11	37.88632768361582	39.0	38.0	39.0	35.0	39.0
12	37.68335216572505	39.0	38.0	39.0	35.0	39.0
13	37.826214689265534	39.0	38.0	39.0	35.0	39.0
14	39.13412429378531	40.0	38.0	41.0	36.0	41.0
15	39.110583804143126	40.0	38.0	41.0	36.0	41.0
16	38.97306967984934	40.0	38.0	41.0	36.0	41.0
17	39.06809792843691	40.0	38.0	41.0	36.0	41.0
18	39.08418079096045	40.0	39.0	41.0	36.0	41.0
19	39.093032015065916	40.0	39.0	41.0	36.0	41.0
20	39.067156308851224	40.0	39.0	41.0	36.0	41.0
21	39.055329566854994	40.0	39.0	41.0	36.0	41.0
22	38.97077212806026	40.0	39.0	41.0	35.0	41.0
23	38.89291902071563	40.0	38.0	41.0	35.0	41.0
24	38.79555555555555	40.0	38.0	41.0	35.0	41.0
25	38.776346516007536	40.0	38.0	41.0	35.0	41.0
26	38.552956685499055	40.0	38.0	41.0	35.0	41.0
27	38.4425988700565	40.0	38.0	41.0	35.0	41.0
28	38.31084745762712	40.0	38.0	41.0	34.0	41.0
29	38.11728813559322	40.0	37.0	41.0	34.0	41.0
30	37.88444444444445	40.0	37.0	41.0	33.0	41.0
31	37.63996233521657	40.0	36.0	41.0	33.0	41.0
32	37.44421845574388	39.0	35.0	41.0	33.0	41.0
33	37.229491525423725	39.0	35.0	41.0	33.0	41.0
34	37.01792843691149	39.0	35.0	41.0	33.0	41.0
35	36.831487758945386	39.0	35.0	41.0	32.0	41.0
36	36.65902071563089	39.0	35.0	41.0	32.0	41.0
37	36.47133709981168	39.0	35.0	41.0	31.0	41.0
38	36.21623352165725	38.0	35.0	41.0	31.0	41.0
39	35.9305461393597	38.0	35.0	40.0	30.0	41.0
40	35.64787193973635	38.0	35.0	40.0	29.0	41.0
41	35.30606403013183	38.0	35.0	40.0	27.0	41.0
42	34.957024482109226	37.0	35.0	40.0	25.0	41.0
43	33.86964218455744	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	0.0
19	3.0
20	8.0
21	6.0
22	20.0
23	22.0
24	47.0
25	74.0
26	116.0
27	156.0
28	252.0
29	297.0
30	368.0
31	477.0
32	597.0
33	687.0
34	978.0
35	1430.0
36	2416.0
37	4727.0
38	6864.0
39	7002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.97928436911488	16.278719397363464	13.529190207156308	23.21280602636535
2	21.26553672316384	21.03201506591337	31.001883239171374	26.70056497175141
3	23.461393596986817	22.09416195856874	28.052730696798495	26.39171374764595
4	15.913370998116761	16.354048964218457	29.480225988700564	38.25235404896422
5	16.768361581920903	36.610169491525426	28.214689265536723	18.40677966101695
6	32.62146892655367	30.595103578154426	17.182674199623353	19.60075329566855
7	30.22975517890772	30.824858757062145	17.672316384180792	21.27306967984934
8	33.536723163841806	28.112994350282488	17.21280602636535	21.137476459510356
9	30.22222222222222	12.112994350282486	18.451977401129945	39.21280602636535
10	22.674199623352166	25.397363465160076	24.199623352165727	27.728813559322035
11	36.60640301318268	22.372881355932204	16.651600753295668	24.369114877589453
12	24.24105461393597	28.00376647834275	23.642184557438796	24.112994350282484
13	34.81732580037665	17.53295668549906	23.465160075329567	24.184557438794727
14	25.423728813559322	20.16949152542373	23.84180790960452	30.56497175141243
15	31.81544256120527	22.94538606403013	20.64783427495292	24.591337099811675
16	25.499058380414315	23.838041431261768	24.63653483992467	26.026365348399246
17	23.145009416195858	27.34839924670433	23.800376647834277	25.70621468926554
18	23.728813559322035	21.397363465160073	27.54802259887006	27.325800376647834
19	25.077212806026367	25.58568738229755	28.015065913370996	21.322033898305083
20	26.188323917137474	21.35593220338983	28.900188323917135	23.555555555555554
21	28.20715630885122	20.994350282485875	27.21280602636535	23.58568738229755
22	27.76647834274953	22.139359698681734	25.30320150659134	24.7909604519774
23	25.141242937853107	23.657250470809792	26.734463276836156	24.46704331450094
24	24.448210922787194	23.804143126177024	27.35216572504708	24.3954802259887
25	24.433145009416197	23.306967984934087	26.109227871939737	26.15065913370998
26	25.167608286252353	23.156308851224104	26.399246704331453	25.27683615819209
27	24.80979284369115	23.31450094161959	26.09416195856874	25.781544256120526
28	24.41431261770245	23.242937853107346	27.841807909604523	24.500941619585685
29	24.282485875706215	23.5969868173258	28.444444444444443	23.67608286252354
30	23.35216572504708	24.512241054613938	28.839924670433142	23.295668549905837
31	23.630885122410547	25.009416195856875	27.66854990583804	23.69114877589454
32	22.926553672316384	24.922787193973633	28.549905838041433	23.60075329566855
33	23.12241054613936	24.282485875706215	28.65536723163842	23.939736346516007
34	23.450094161958567	24.753295668549903	27.220338983050844	24.576271186440678
35	22.493408662900187	24.794726930320152	27.93596986817326	24.7758945386064
36	21.34463276836158	24.772128060263654	28.045197740112993	25.83804143126177
37	22.128060263653484	24.523540489642183	28.267419962335218	25.080979284369114
38	22.0225988700565	23.35969868173258	29.77024482109228	24.847457627118644
39	21.947269303201505	22.95668549905838	30.433145009416197	24.662900188323917
40	21.51789077212806	22.839924670433145	30.207156308851225	25.43502824858757
41	19.713747645951035	23.145009416195858	30.96421845574388	26.17702448210923
42	19.811676082862522	23.604519774011298	30.436911487758945	26.146892655367232
43	18.62146892655367	24.203389830508474	31.325800376647834	25.84934086629002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	6.0
2	6.0
3	23.5
4	41.0
5	41.0
6	60.5
7	80.0
8	79.5
9	79.0
10	142.0
11	205.0
12	205.0
13	333.0
14	461.0
15	699.0
16	937.0
17	823.5
18	710.0
19	710.0
20	762.0
21	814.0
22	579.0
23	344.0
24	301.0
25	258.0
26	258.0
27	248.5
28	239.0
29	246.5
30	254.0
31	254.5
32	255.0
33	255.0
34	284.0
35	313.0
36	357.0
37	401.0
38	469.0
39	537.0
40	537.0
41	618.5
42	700.0
43	824.5
44	949.0
45	1177.5
46	1406.0
47	1406.0
48	1722.5
49	2039.0
50	1944.5
51	1850.0
52	1704.0
53	1558.0
54	1558.0
55	1476.5
56	1395.0
57	1464.0
58	1533.0
59	1513.0
60	1493.0
61	1493.0
62	1524.5
63	1556.0
64	1508.5
65	1461.0
66	1386.0
67	1311.0
68	1311.0
69	1202.5
70	1094.0
71	974.0
72	854.0
73	721.0
74	588.0
75	588.0
76	488.0
77	388.0
78	319.0
79	250.0
80	181.0
81	112.0
82	112.0
83	81.0
84	50.0
85	32.0
86	14.0
87	11.0
88	8.0
89	8.0
90	4.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26550.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.2090395480226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.94276703306896	76.29001883239171
2	3.6501089930893746	5.928436911487759
3	1.015722832892723	2.4745762711864403
4	0.33857427763090764	1.0998116760828625
5	0.24581420156764527	0.9981167608286252
6	0.16696813691387227	0.8135593220338984
7	0.08812207226009926	0.5009416195856874
8	0.07420806085060991	0.4821092278719397
9	0.07420806085060991	0.5423728813559322
>10	0.36640230044988636	4.862523540489643
>50	0.018552015212652477	0.8286252354048964
>100	0.018552015212652477	5.178907721280603
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	1.7853107344632766	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	421	1.585687382297552	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	330	1.2429378531073447	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	150	0.5649717514124294	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57	0.21468926553672316	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	56	0.21092278719397364	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	54	0.2033898305084746	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.19962335216572505	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	38	0.1431261770244821	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	37	0.13935969868173256	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	35	0.1318267419962335	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.12429378531073447	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30	0.11299435028248588	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	28	0.10546139359698682	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28	0.10546139359698682	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	27	0.1016949152542373	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	27	0.1016949152542373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.003766478342749529	0.0
17	0.0	0.0	0.0	0.007532956685499058	0.0
18	0.0	0.0	0.0	0.007532956685499058	0.0
19	0.0	0.0	0.0	0.007532956685499058	0.0
20	0.0	0.0	0.0	0.007532956685499058	0.0
21	0.0	0.0	0.0	0.007532956685499058	0.0
22	0.0	0.0	0.0	0.018832391713747645	0.0
23	0.0	0.0	0.0	0.018832391713747645	0.0
24	0.0	0.0	0.0	0.03766478342749529	0.0
25	0.0	0.0	0.0	0.060263653483992465	0.0
26	0.0	0.0	0.0	0.07909604519774012	0.0
27	0.0	0.0	0.0	0.16195856873822975	0.0
28	0.0	0.0	0.0	0.3540489642184557	0.0
29	0.0	0.0	0.0	0.704331450094162	0.0
30	0.0	0.0	0.0	1.1713747645951036	0.0
31	0.0	0.0	0.0	1.9096045197740112	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCTTA	135	1.9506842E-8	16.444445	37
ACTGTCT	75	0.0039657345	14.8	37
GGTATCA	285	0.0	14.280702	1
GTCTCTT	175	4.68277E-7	12.685715	36
CTCTATG	110	0.0035828012	11.772727	1
TCTATGG	115	0.0049940026	11.260869	2
AACGCAG	350	1.8189894E-12	11.1	7
GTATCAA	370	0.0	11.0	2
TATGGGC	120	0.006856514	10.791667	4
ATCAACG	380	1.8189894E-12	10.710526	4
TGTCTCT	245	2.0791231E-7	10.571428	37
TCAACGC	390	1.8189894E-12	10.435898	5
CAACGCA	390	1.8189894E-12	10.435898	6
CCTGTCT	160	4.4973142E-4	10.40625	36
CTATGGG	125	0.009283763	10.36	3
TATCAAC	405	3.6379788E-12	10.049382	3
ATGGGAA	155	0.0040981383	9.548388	19
GAGTACA	270	8.369681E-6	8.907408	6
CGCAGAG	440	2.0190782E-10	8.829545	2
AGTACAT	275	1.0559639E-5	8.745454	7
>>END_MODULE