FastQCFastQC Report
Fri 10 Feb 2017
ERR1631294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287613
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA41930.1832914920486988No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC38870.16991510364733894No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC29130.1273379719384354No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC28530.1247151506832668No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG27210.11894494392189588No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC25850.11299988241018039No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA25030.10941536002811664No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT24410.10670511139777576No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC23010.1005851951357157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.022.0651051
GCAGTCG7050.020.4680849
AGTCGGT7000.020.3511
CAGTCGG7400.019.510
TAGCGAA1052.2622771E-819.38095310
CGGTGAT7350.019.38095314
CTTATAC29450.019.34804737
CCGCTTA2150.018.93023325
TCGGTGA7850.018.85350413
CGCTTAT2100.018.50000226
CTCTATG10450.018.4114841
CTTATTG7500.018.00666628
CGCCTTA5300.017.80188625
TCGGGTA1156.413029E-817.69565225
TTATTGA7850.017.20382129
TCTATGG10900.017.1422022
TGTGCGA650.001580580417.07692310
TATTAGA1757.2759576E-1216.9142862
GCCTTAT5700.016.87719326
GTCGGTG8450.016.85798612
Produced by FastQC (version 0.11.5)