##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631294.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2287613
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87693198106498	34.0	33.0	34.0	31.0	34.0
2	33.05274012693581	34.0	33.0	34.0	31.0	34.0
3	33.16535664030586	34.0	33.0	34.0	31.0	34.0
4	36.48429345348186	37.0	37.0	37.0	35.0	37.0
5	36.46752138582881	37.0	37.0	37.0	35.0	37.0
6	36.50572408882097	37.0	37.0	37.0	35.0	37.0
7	36.49950494248809	37.0	37.0	37.0	35.0	37.0
8	36.47989498223694	37.0	37.0	37.0	35.0	37.0
9	38.344501889087006	39.0	39.0	39.0	37.0	39.0
10	38.31412262476214	39.0	39.0	39.0	37.0	39.0
11	38.36752501406488	39.0	39.0	39.0	37.0	39.0
12	38.148477474118216	39.0	39.0	39.0	37.0	39.0
13	38.26692320772788	39.0	39.0	39.0	37.0	39.0
14	39.82740830726176	41.0	40.0	41.0	38.0	41.0
15	39.84226658967229	41.0	40.0	41.0	38.0	41.0
16	39.82330840050306	41.0	40.0	41.0	38.0	41.0
17	39.81121194887422	41.0	40.0	41.0	38.0	41.0
18	39.783116287588854	41.0	40.0	41.0	38.0	41.0
19	39.80617044928491	41.0	40.0	41.0	38.0	41.0
20	39.78158062574395	41.0	40.0	41.0	38.0	41.0
21	39.75457824378512	41.0	40.0	41.0	38.0	41.0
22	39.7140748894153	41.0	40.0	41.0	38.0	41.0
23	39.65850430120829	41.0	40.0	41.0	37.0	41.0
24	39.631882228331456	41.0	40.0	41.0	37.0	41.0
25	39.615279769786234	41.0	40.0	41.0	37.0	41.0
26	39.55382444495638	41.0	40.0	41.0	37.0	41.0
27	39.49098558191442	41.0	39.0	41.0	37.0	41.0
28	39.40888777953264	41.0	39.0	41.0	37.0	41.0
29	39.35410316342843	41.0	39.0	41.0	36.0	41.0
30	39.305399558404325	41.0	39.0	41.0	36.0	41.0
31	39.265152803380644	41.0	39.0	41.0	36.0	41.0
32	39.193368808447936	41.0	39.0	41.0	35.0	41.0
33	39.111753168040224	41.0	39.0	41.0	35.0	41.0
34	39.07641895722747	41.0	39.0	41.0	35.0	41.0
35	38.96877881005222	40.0	39.0	41.0	35.0	41.0
36	38.913355099835506	40.0	39.0	41.0	35.0	41.0
37	38.85410906477625	40.0	38.0	41.0	35.0	41.0
38	38.756599127562225	40.0	38.0	41.0	35.0	41.0
39	38.6834626311356	40.0	38.0	41.0	35.0	41.0
40	38.60120439952038	40.0	38.0	41.0	35.0	41.0
41	38.52243014880576	40.0	38.0	41.0	35.0	41.0
42	38.42710545883416	40.0	38.0	41.0	35.0	41.0
43	37.512804394799296	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	2.0
13	1.0
14	2.0
15	2.0
16	11.0
17	22.0
18	71.0
19	102.0
20	171.0
21	315.0
22	493.0
23	893.0
24	1373.0
25	2107.0
26	2963.0
27	4176.0
28	5929.0
29	8523.0
30	11336.0
31	15166.0
32	20483.0
33	27995.0
34	41770.0
35	62111.0
36	103722.0
37	195378.0
38	474825.0
39	1307667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.114329215649676	17.24491861167077	12.524277489243154	27.116474683436405
2	19.160364974320395	19.272315728228506	34.286306293940456	27.281013003510644
3	19.938687181791675	20.514396447301184	28.81553829253462	30.731378078372522
4	14.791007045335029	14.999084198245068	32.74649164871855	37.463417107701346
5	16.138437751490308	34.716536407163275	32.680440266775896	16.464585574570524
6	35.95289063316216	33.36202408361904	15.483125860886435	15.201959422332362
7	31.610023198854005	29.02545142032328	19.544564574515007	19.81996080630771
8	28.2152619346017	32.88773057330938	18.985379082913063	19.91162840917585
9	27.212775937188677	14.742091428926136	17.98354879081383	40.06158384307136
10	18.493993520757225	25.042609916974595	30.116501348785828	26.34689521348235
11	38.146880613110696	20.75652656284083	19.805710144154627	21.290882679893848
12	23.64276649940353	24.053238025837413	26.460550801206324	25.84344467355274
13	31.01022769148453	17.373567994236787	24.611986380563497	27.004217933715186
14	24.156183760102778	19.137633856775597	22.323268839615793	34.38291354350583
15	27.055362948190975	25.659716044628176	20.40926502865651	26.87565597852434
16	27.566419669760577	24.37344078740591	21.674339147399493	26.385800395434018
17	26.388991494627806	24.838904132823163	22.496768465645196	26.275335906903834
18	26.02861585416764	22.924856608176295	24.416629910741023	26.629897626915046
19	26.87460685002227	23.83021079177291	23.344464295315685	25.950718062889134
20	27.053308404874425	22.54966202762443	23.57168804338846	26.825341524112687
21	28.04967448602539	23.463365525549996	22.471239672094885	26.015720316329727
22	27.808156362111948	22.730505553168303	22.385123707550182	27.076214377169567
23	25.941713043246388	23.183291929185575	23.47884017095549	27.396154856612547
24	26.376664234728516	23.809184508043973	23.266041939786145	26.548109317441366
25	26.74936713508797	22.797824632050965	23.162877637082847	27.289930595778216
26	26.81034772927064	23.95204083907549	23.277932062809576	25.95967936884429
27	27.667879138648015	22.42223662831082	22.805168531565435	27.10471570147573
28	25.99290177140976	23.270107312731657	23.794584136390203	26.94240677946838
29	25.537842283638014	23.638395130644913	24.04077962487536	26.782982960841718
30	24.850357118970734	24.823473201105255	24.09371690054218	26.232452779381827
31	26.79010829191826	23.640537101336633	22.919304969852856	26.650049636892252
32	25.3665720556755	23.093635155946394	23.68363879729657	27.856153991081534
33	25.338726436683128	22.789781313535112	24.105257314064925	27.766234935716838
34	26.624345988591603	22.529422590272045	24.145473906644174	26.700757514492178
35	26.485249034692494	22.515390496556893	24.59913455641317	26.400225912337444
36	24.974460278027795	23.508958901702343	24.70138087167716	26.815199948592706
37	26.706396580190795	21.504292902689397	24.724243130284712	27.0650673868351
38	25.009387514409127	21.550760552593466	26.227294564246662	27.212557368750744
39	25.62203484592892	21.11659620748789	26.107912483448903	27.15345646313428
40	24.71029846394473	21.406986234122645	26.976328601035227	26.9063867008974
41	23.33445386085846	21.845172238486143	28.25005802992027	26.57031587073513
42	24.796895279052883	21.330662135597237	27.628580533508075	26.24386205184181
43	22.91235449352666	21.017147568229415	28.035074114371618	28.035423823872307
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	27.5
2	39.0
3	123.0
4	207.0
5	207.0
6	285.0
7	363.0
8	361.5
9	360.0
10	576.0
11	792.0
12	792.0
13	1263.5
14	1735.0
15	3230.5
16	4726.0
17	4615.5
18	4505.0
19	4505.0
20	5278.0
21	6051.0
22	6626.0
23	7201.0
24	8458.0
25	9715.0
26	9715.0
27	11737.5
28	13760.0
29	19905.0
30	26050.0
31	30555.5
32	35061.0
33	35061.0
34	41856.5
35	48652.0
36	57290.5
37	65929.0
38	81913.5
39	97898.0
40	97898.0
41	107523.5
42	117149.0
43	119039.0
44	120929.0
45	134010.0
46	147091.0
47	147091.0
48	159283.0
49	171475.0
50	181604.0
51	191733.0
52	206827.5
53	221922.0
54	221922.0
55	201130.5
56	180339.0
57	171086.0
58	161833.0
59	152799.0
60	143765.0
61	143765.0
62	136052.0
63	128339.0
64	119276.0
65	110213.0
66	99404.5
67	88596.0
68	88596.0
69	75368.0
70	62140.0
71	54441.5
72	46743.0
73	36529.0
74	26315.0
75	26315.0
76	21165.0
77	16015.0
78	13574.5
79	11134.0
80	9314.5
81	7495.0
82	7495.0
83	6169.0
84	4843.0
85	3991.5
86	3140.0
87	2583.0
88	2026.0
89	2026.0
90	1540.0
91	1054.0
92	615.5
93	177.0
94	119.0
95	61.0
96	61.0
97	41.0
98	21.0
99	13.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2287613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.08862729280279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.9446260023159	38.75125209637749
2	7.6643751200012975	6.911523064359384
3	2.205609211247301	2.9834367503850685
4	1.0266347470729555	1.851582059064534
5	0.5979024017162474	1.3479299274227765
6	0.40679451095835695	1.1005083653615604
7	0.27702761757371747	0.8743556499016116
8	0.23296077407238025	0.8403105212793912
9	0.1641074604966065	0.6659442110069862
>10	1.1294559321754158	10.168482247516216
>50	0.1503730530264565	4.773201197856217
>100	0.16832001126798854	16.089210418872984
>500	0.02101419615988361	6.40137991461784
>1k	0.010798961915495743	7.240883575978047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	4193	0.1832914920486988	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3887	0.16991510364733894	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2913	0.1273379719384354	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2853	0.1247151506832668	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2721	0.11894494392189588	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2585	0.11299988241018039	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2503	0.10941536002811664	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2441	0.10670511139777576	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2301	0.1005851951357157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.371368758614329E-5	0.0
2	0.0	0.0	0.0	4.371368758614329E-5	0.0
3	0.0	0.0	0.0	4.371368758614329E-5	0.0
4	0.0	0.0	0.0	8.742737517228658E-5	0.0
5	0.0	4.371368758614329E-5	0.0	1.3114106275842987E-4	0.0
6	0.0	4.371368758614329E-5	0.0	1.3114106275842987E-4	0.0
7	0.0	1.3114106275842987E-4	0.0	1.3114106275842987E-4	0.0
8	0.0	1.3114106275842987E-4	0.0	2.6228212551685973E-4	0.0
9	0.0	1.3114106275842987E-4	0.0	3.497095006891463E-4	4.371368758614329E-5
10	0.0	1.3114106275842987E-4	0.0	5.245642510337195E-4	4.371368758614329E-5
11	0.0	1.7485475034457315E-4	0.0	5.245642510337195E-4	4.371368758614329E-5
12	0.0	2.1856843793071641E-4	0.0	5.245642510337195E-4	4.371368758614329E-5
13	0.0	2.1856843793071641E-4	0.0	5.245642510337195E-4	4.371368758614329E-5
14	0.0	2.1856843793071641E-4	0.0	5.245642510337195E-4	4.371368758614329E-5
15	0.0	2.1856843793071641E-4	0.0	5.682779386198627E-4	4.371368758614329E-5
16	0.0	2.1856843793071641E-4	0.0	6.11991626206006E-4	8.742737517228658E-5
17	0.0	2.1856843793071641E-4	0.0	6.994190013782926E-4	8.742737517228658E-5
18	0.0	2.1856843793071641E-4	0.0	7.868463765505791E-4	8.742737517228658E-5
19	0.0	2.1856843793071641E-4	0.0	8.742737517228657E-4	8.742737517228658E-5
20	0.0	2.1856843793071641E-4	0.0	0.0011802695648258687	8.742737517228658E-5
21	4.371368758614329E-5	2.1856843793071641E-4	0.0	0.0014862653779288716	8.742737517228658E-5
22	4.371368758614329E-5	2.1856843793071641E-4	0.0	0.002098257004134878	8.742737517228658E-5
23	4.371368758614329E-5	2.6228212551685973E-4	4.371368758614329E-5	0.0034533813193053198	8.742737517228658E-5
24	4.371368758614329E-5	3.05995813103003E-4	4.371368758614329E-5	0.005289356197923338	8.742737517228658E-5
25	4.371368758614329E-5	3.05995813103003E-4	4.371368758614329E-5	0.006688194200679923	8.742737517228658E-5
26	4.371368758614329E-5	3.05995813103003E-4	4.371368758614329E-5	0.010185289207571386	8.742737517228658E-5
27	4.371368758614329E-5	3.05995813103003E-4	4.371368758614329E-5	0.030993004498575588	8.742737517228658E-5
28	4.371368758614329E-5	3.05995813103003E-4	4.371368758614329E-5	0.13844124858531578	8.742737517228658E-5
29	4.371368758614329E-5	4.3713687586143283E-4	4.371368758614329E-5	0.2929691342023323	8.742737517228658E-5
30	4.371368758614329E-5	4.8085056344757615E-4	4.371368758614329E-5	0.5095267425040861	8.742737517228658E-5
31	4.371368758614329E-5	4.8085056344757615E-4	4.371368758614329E-5	1.148052577074881	8.742737517228658E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1920	0.0	22.065105	1
GCAGTCG	705	0.0	20.468084	9
AGTCGGT	700	0.0	20.35	11
CAGTCGG	740	0.0	19.5	10
TAGCGAA	105	2.2622771E-8	19.380953	10
CGGTGAT	735	0.0	19.380953	14
CTTATAC	2945	0.0	19.348047	37
CCGCTTA	215	0.0	18.930233	25
TCGGTGA	785	0.0	18.853504	13
CGCTTAT	210	0.0	18.500002	26
CTCTATG	1045	0.0	18.411484	1
CTTATTG	750	0.0	18.006666	28
CGCCTTA	530	0.0	17.801886	25
TCGGGTA	115	6.413029E-8	17.695652	25
TTATTGA	785	0.0	17.203821	29
TCTATGG	1090	0.0	17.142202	2
TGTGCGA	65	0.0015805804	17.076923	10
TATTAGA	175	7.2759576E-12	16.914286	2
GCCTTAT	570	0.0	16.877193	26
GTCGGTG	845	0.0	16.857986	12
>>END_MODULE