FastQCFastQC Report
Fri 10 Feb 2017
ERR1631293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741047
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA33730.19373400028833226No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT30790.1768476095131263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30330.17420552116054305No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC27820.15978890862796927No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC27350.157089383572069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26350.15134571324036628No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC25210.1447979290622252No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT24020.13796296136749897No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG23210.1333105883988198No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC22960.13187467081589413No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC22760.13072593674955357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21700.1246376461979487No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT20860.11981296311931844No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG20440.1174006215800033No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG20040.11510315344732222No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA19890.11424160289756681No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC18910.10861280597249814No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA18600.10683226816967033No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG18230.10470711014694031No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC17840.10246707871757627No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17440.10016961058489518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.022.9655171
ATCGGTA705.1044117E-621.14285916
GACTTAT609.239421E-418.51
TCGTTTA6250.017.7630
CTTATAC14500.017.60689537
CGGTGAT3250.017.07692314
TCTATGG4950.016.8181822
GTGCTAG904.448073E-516.4444451
AGTCGGT3400.016.3235311
TCGGGTA803.3838165E-416.187525
GTCTTAT803.3838165E-416.18751
CGTTTAT6900.016.08695631
TCGGTGA3450.016.08695613
TCTAGCG6600.015.97727328
AAGACGG7600.015.8223695
GCCTTAT2600.015.65384526
TAGCGAA957.063011E-515.57894810
CTCTATG5650.015.389381
CAAGACG7850.015.3184714
TACACCG855.366121E-415.2352945
Produced by FastQC (version 0.11.5)