##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631293.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1741047
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.623883789466916	34.0	31.0	34.0	31.0	34.0
2	32.81806234983892	34.0	31.0	34.0	31.0	34.0
3	32.927236886769855	34.0	31.0	34.0	31.0	34.0
4	36.33526665276698	37.0	37.0	37.0	35.0	37.0
5	36.268073176657495	37.0	37.0	37.0	35.0	37.0
6	36.29805858199118	37.0	37.0	37.0	35.0	37.0
7	36.29376748588636	37.0	37.0	37.0	35.0	37.0
8	36.277721394080686	37.0	37.0	37.0	35.0	37.0
9	38.08326426569759	39.0	38.0	39.0	37.0	39.0
10	38.012326490898865	39.0	38.0	39.0	35.0	39.0
11	38.0788973531444	39.0	38.0	39.0	37.0	39.0
12	37.84375263849856	39.0	38.0	39.0	35.0	39.0
13	37.97938194661029	39.0	38.0	39.0	35.0	39.0
14	39.42961964840696	40.0	39.0	41.0	37.0	41.0
15	39.411872281449035	40.0	39.0	41.0	37.0	41.0
16	39.39148167740446	40.0	39.0	41.0	37.0	41.0
17	39.374807802431526	40.0	39.0	41.0	37.0	41.0
18	39.319113154326104	40.0	39.0	41.0	36.0	41.0
19	39.32029864788257	40.0	39.0	41.0	36.0	41.0
20	39.2957662831618	40.0	39.0	41.0	36.0	41.0
21	39.26300725942493	40.0	39.0	41.0	36.0	41.0
22	39.20345745979287	40.0	39.0	41.0	36.0	41.0
23	39.114927971502205	40.0	39.0	41.0	36.0	41.0
24	39.06113218080844	40.0	39.0	41.0	35.0	41.0
25	39.051386320989614	40.0	39.0	41.0	35.0	41.0
26	38.95533721950068	40.0	39.0	41.0	35.0	41.0
27	38.8761285594243	40.0	38.0	41.0	35.0	41.0
28	38.75430243985372	40.0	38.0	41.0	35.0	41.0
29	38.66030497740727	40.0	38.0	41.0	35.0	41.0
30	38.596609396529786	40.0	38.0	41.0	35.0	41.0
31	38.54972266687803	40.0	38.0	41.0	35.0	41.0
32	38.467385429571976	40.0	38.0	41.0	34.0	41.0
33	38.33239883817036	40.0	38.0	41.0	34.0	41.0
34	38.28016073087056	40.0	38.0	41.0	34.0	41.0
35	38.140005410537455	40.0	38.0	41.0	34.0	41.0
36	38.07829713959474	40.0	37.0	41.0	33.0	41.0
37	38.00363229711777	40.0	37.0	41.0	33.0	41.0
38	37.899450158439144	40.0	37.0	41.0	33.0	41.0
39	37.8060414222017	40.0	37.0	41.0	33.0	41.0
40	37.67752048049248	40.0	36.0	41.0	33.0	41.0
41	37.562649371326565	40.0	36.0	41.0	33.0	41.0
42	37.4250436662537	40.0	36.0	41.0	33.0	41.0
43	36.42657205692896	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	2.0
14	4.0
15	7.0
16	25.0
17	40.0
18	75.0
19	136.0
20	237.0
21	438.0
22	823.0
23	1253.0
24	1887.0
25	2756.0
26	3912.0
27	5498.0
28	7784.0
29	10283.0
30	13748.0
31	18012.0
32	24201.0
33	33084.0
34	49262.0
35	72231.0
36	114548.0
37	210958.0
38	467893.0
39	701947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97244876215289	17.427444520452347	11.50784556648959	27.092261150905173
2	20.665094049729845	19.323602407057365	31.72901133628213	28.28229220693066
3	20.92757978388866	20.80908786494563	28.4298470977521	29.833485253413606
4	15.176442680754743	14.852269927233442	32.50216679963264	37.469120592379184
5	17.060481422959864	34.02464149445707	31.480712467842626	17.434164614740443
6	36.30855456515533	32.91909982901093	15.234511187808256	15.537834418025476
7	31.404953456167465	28.714503399391283	19.473512202714804	20.407030941726447
8	29.839688417371846	29.780011682625457	18.82344359457269	21.55685630543001
9	28.005389860239273	13.275804731291	18.42546467728901	40.29334073118072
10	19.705154427192372	23.767882199618963	29.176064747246915	27.350898625941745
11	39.54557229069635	19.808023562833167	19.319064907495317	21.327339238975167
12	24.704157900389824	23.473691405229154	25.433948652735967	26.388202041645055
13	31.76772367431781	16.751069902191038	23.32131183132908	28.159894592162072
14	24.64959303223865	19.161458593593395	21.84105311344266	34.347895260725295
15	28.033074351238074	25.173817823413152	19.59895396275919	27.19415386258958
16	28.143467695013403	23.44439868653747	21.28110269280496	27.13103092564417
17	26.877218133686227	23.7123409075114	22.315595156247937	27.094845802554442
18	27.16646937159077	21.279839085331986	24.020603694213886	27.533087848863357
19	27.15860054323634	22.858716622813745	22.880485133370897	27.102197700579016
20	27.29449578328443	21.740998376264397	22.666303666701705	28.298202173749477
21	29.609022616850666	22.43316808793789	22.207154660385388	25.750654634826052
22	29.31012201278886	21.945415603369696	22.048054992197226	26.69640739164422
23	27.45864988136449	21.76000992506233	22.723223439688876	28.0581167538843
24	26.94240879195105	22.914717408547844	22.71604385177425	27.426829947726855
25	27.297425055153596	21.614465318856986	22.738156982551306	28.349952643438115
26	27.26210148261362	23.217868328655115	23.046706952770375	26.473323235960887
27	28.626280623096335	21.543243806743874	22.40243945166328	27.428036118496514
28	26.545233988513807	22.072752774623545	23.96201825683052	27.41999498003213
29	26.093207133408807	22.48957093059521	24.15431633953592	27.26290559646006
30	25.54187221826866	23.22280788514038	24.26585841737759	26.96946147921337
31	27.17485513027506	23.080364860914152	22.205661306099145	27.539118702711647
32	26.379241915927597	22.328863034714168	22.885999056889332	28.4058959924689
33	25.845884688925686	22.135818274865642	23.63233157979078	28.38596545641789
34	27.48064813873491	22.10009264540245	23.68086559409367	26.738393621768967
35	27.50833262973372	21.84432700553173	24.406808087317575	26.24053227741698
36	25.531993105298135	23.388283027396735	23.51194424963829	27.567779617666844
37	26.43639143572804	22.049261162966882	24.33926252421675	27.17508487708833
38	25.75634086845444	21.446635271764634	25.285302464551503	27.511721395229422
39	25.955301608744623	21.396033536142333	25.063309606231194	27.58535524888185
40	25.471799440221886	21.52744871333169	26.16678355035792	26.8339682960885
41	24.097568876658702	21.87040326883766	27.176520794671255	26.85550705983239
42	25.30465863356934	22.113532833978635	26.59830550237874	25.983503030073287
43	24.02973613004129	20.386870658862165	27.1469983291663	28.436394881930237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	122.0
1	113.0
2	104.0
3	239.0
4	374.0
5	374.0
6	483.5
7	593.0
8	574.0
9	555.0
10	788.0
11	1021.0
12	1021.0
13	1850.0
14	2679.0
15	4258.5
16	5838.0
17	5487.0
18	5136.0
19	5136.0
20	5943.0
21	6750.0
22	6177.0
23	5604.0
24	6821.5
25	8039.0
26	8039.0
27	9596.0
28	11153.0
29	13818.0
30	16483.0
31	19937.5
32	23392.0
33	23392.0
34	29287.5
35	35183.0
36	43459.0
37	51735.0
38	58364.0
39	64993.0
40	64993.0
41	72700.0
42	80407.0
43	84098.0
44	87789.0
45	94189.0
46	100589.0
47	100589.0
48	106671.0
49	112753.0
50	116102.0
51	119451.0
52	127532.5
53	135614.0
54	135614.0
55	135232.5
56	134851.0
57	130372.5
58	125894.0
59	123336.5
60	120779.0
61	120779.0
62	116402.0
63	112025.0
64	104555.0
65	97085.0
66	86313.5
67	75542.0
68	75542.0
69	65649.0
70	55756.0
71	49927.0
72	44098.0
73	38278.0
74	32458.0
75	32458.0
76	28535.0
77	24612.0
78	20572.0
79	16532.0
80	12746.5
81	8961.0
82	8961.0
83	7372.0
84	5783.0
85	5144.5
86	4506.0
87	3982.0
88	3458.0
89	3458.0
90	2617.5
91	1777.0
92	1070.0
93	363.0
94	254.0
95	145.0
96	145.0
97	99.0
98	53.0
99	32.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1741047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.28638832483166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.00563155908306	39.40170817231864
2	7.013576483890419	6.352390963907379
3	1.9772865472397836	2.6863249922329935
4	0.9543448601620131	1.7287532773241638
5	0.5565479371251	1.2602023001015636
6	0.3771771805467134	1.0248595359302164
7	0.2660405180554177	0.8433609947557924
8	0.19938088987686556	0.7223392322811386
9	0.15222443821138198	0.6204325519232988
>10	1.1388932840291284	10.308879674808505
>50	0.1630183729535366	5.224588399314719
>100	0.16278622280206462	15.492014085983872
>500	0.022527815255173753	6.911099844366511
>1k	0.010563890769375263	7.423045974751348
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3373	0.19373400028833226	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3079	0.1768476095131263	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3033	0.17420552116054305	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2782	0.15978890862796927	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2735	0.157089383572069	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2635	0.15134571324036628	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2521	0.1447979290622252	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2402	0.13796296136749897	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	2321	0.1333105883988198	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2296	0.13187467081589413	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2276	0.13072593674955357	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2170	0.1246376461979487	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2086	0.11981296311931844	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	2044	0.1174006215800033	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	2004	0.11510315344732222	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1989	0.11424160289756681	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1891	0.10861280597249814	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	1860	0.10683226816967033	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1823	0.10470711014694031	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1784	0.10246707871757627	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1744	0.10016961058489518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.743670331702705E-5	0.0	0.0	0.0
5	0.0	5.743670331702705E-5	0.0	0.0	0.0
6	0.0	5.743670331702705E-5	0.0	0.0	5.743670331702705E-5
7	0.0	5.743670331702705E-5	0.0	0.0	5.743670331702705E-5
8	0.0	5.743670331702705E-5	0.0	0.0	1.148734066340541E-4
9	0.0	5.743670331702705E-5	0.0	0.0	1.148734066340541E-4
10	0.0	5.743670331702705E-5	0.0	5.743670331702705E-5	1.148734066340541E-4
11	0.0	5.743670331702705E-5	0.0	5.743670331702705E-5	1.148734066340541E-4
12	0.0	5.743670331702705E-5	0.0	1.148734066340541E-4	1.148734066340541E-4
13	0.0	5.743670331702705E-5	0.0	1.7231010995108115E-4	1.148734066340541E-4
14	0.0	5.743670331702705E-5	0.0	2.297468132681082E-4	1.148734066340541E-4
15	0.0	5.743670331702705E-5	0.0	3.446202199021623E-4	1.148734066340541E-4
16	0.0	1.7231010995108115E-4	0.0	7.466771431213517E-4	1.148734066340541E-4
17	5.743670331702705E-5	1.7231010995108115E-4	0.0	9.7642395638946E-4	1.148734066340541E-4
18	5.743670331702705E-5	1.7231010995108115E-4	0.0	0.0012636074729745952	1.148734066340541E-4
19	5.743670331702705E-5	1.7231010995108115E-4	0.0	0.0014359175829256764	1.148734066340541E-4
20	5.743670331702705E-5	1.7231010995108115E-4	0.0	0.0020677213194129737	1.148734066340541E-4
21	1.148734066340541E-4	1.7231010995108115E-4	0.0	0.0026420883525832446	1.7231010995108115E-4
22	1.148734066340541E-4	1.7231010995108115E-4	0.0	0.0035610756056556774	1.7231010995108115E-4
23	1.148734066340541E-4	1.7231010995108115E-4	0.0	0.004365189452094056	1.7231010995108115E-4
24	1.148734066340541E-4	1.7231010995108115E-4	0.0	0.008156011871017842	1.7231010995108115E-4
25	1.148734066340541E-4	1.7231010995108115E-4	0.0	0.010051423080479734	1.7231010995108115E-4
26	1.148734066340541E-4	1.7231010995108115E-4	0.0	0.01562278330223136	1.7231010995108115E-4
27	1.148734066340541E-4	1.7231010995108115E-4	0.0	0.03710411034279948	1.7231010995108115E-4
28	1.148734066340541E-4	2.871835165851353E-4	0.0	0.125269449934436	1.7231010995108115E-4
29	1.148734066340541E-4	2.871835165851353E-4	0.0	0.24812655832955688	1.7231010995108115E-4
30	1.148734066340541E-4	2.871835165851353E-4	0.0	0.40900676432054967	1.7231010995108115E-4
31	1.148734066340541E-4	2.871835165851353E-4	0.0	0.7888931200593666	1.7231010995108115E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1450	0.0	22.965517	1
ATCGGTA	70	5.1044117E-6	21.142859	16
GACTTAT	60	9.239421E-4	18.5	1
TCGTTTA	625	0.0	17.76	30
CTTATAC	1450	0.0	17.606895	37
CGGTGAT	325	0.0	17.076923	14
TCTATGG	495	0.0	16.818182	2
GTGCTAG	90	4.448073E-5	16.444445	1
AGTCGGT	340	0.0	16.32353	11
TCGGGTA	80	3.3838165E-4	16.1875	25
GTCTTAT	80	3.3838165E-4	16.1875	1
CGTTTAT	690	0.0	16.086956	31
TCGGTGA	345	0.0	16.086956	13
TCTAGCG	660	0.0	15.977273	28
AAGACGG	760	0.0	15.822369	5
GCCTTAT	260	0.0	15.653845	26
TAGCGAA	95	7.063011E-5	15.578948	10
CTCTATG	565	0.0	15.38938	1
CAAGACG	785	0.0	15.318471	4
TACACCG	85	5.366121E-4	15.235294	5
>>END_MODULE