FastQCFastQC Report
Fri 10 Feb 2017
ERR1631292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132268
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32972.4926664045725344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30732.3233132730516832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23061.74343000574591No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.8112317416155078No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.28880757250430944No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3640.27519883872138384No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.2555417788127136No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA3310.25024949345268693No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3040.22983639277829862No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.22227598512111774No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.1927903952581123No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG2480.1874981098980857No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.15498835697220795No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.1512081531436175No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG1870.14137962318928238No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1780.13457525629781958No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.11038195179484077No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.1096259110291227No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.10660174796625034No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1400.10584570720053225No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC1390.10508966643481416No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA1380.10433362566909607No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1370.103577584903378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATG200.001836053137.01
TTCTCCT200.001836053137.029
AGTTCGG250.005478990729.610
TATTGTT453.973106E-628.77777713
GTTATTG405.901073E-527.75000211
TTTACCG358.827784E-426.42857230
GTTTACC358.827784E-426.42857229
TTACCGG358.827784E-426.42857231
ATTGTTA509.011921E-625.90000214
TTATTGT451.3145823E-424.66666612
ACCGGGA400.001922213123.12500233
CCGGGAC400.001922213123.12500234
CGGGACT400.001922213123.12500235
CGAGACT400.001922213123.12500210
TACCGGG400.001922213123.12500232
CGTTATT502.6846223E-422.210
TACCTTA855.093898E-821.76470631
GCTACCT855.093898E-821.76470629
TTGTGAT603.6942914E-521.58333434
TGTTACG603.6942914E-521.58333416
Produced by FastQC (version 0.11.5)