##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631292.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132268
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59568451930928	34.0	31.0	34.0	31.0	34.0
2	32.70676958901624	34.0	31.0	34.0	31.0	34.0
3	32.73450116430278	34.0	31.0	34.0	31.0	34.0
4	36.215690870051716	37.0	35.0	37.0	35.0	37.0
5	36.188488523301174	37.0	35.0	37.0	35.0	37.0
6	36.28886805576557	37.0	37.0	37.0	35.0	37.0
7	36.286736020806245	37.0	37.0	37.0	35.0	37.0
8	36.31194998034294	37.0	37.0	37.0	35.0	37.0
9	38.07536214352678	39.0	38.0	39.0	37.0	39.0
10	38.02399673390389	39.0	38.0	39.0	35.0	39.0
11	38.10746363443917	39.0	38.0	39.0	37.0	39.0
12	37.895938549006566	39.0	38.0	39.0	35.0	39.0
13	38.0425877763329	39.0	38.0	39.0	37.0	39.0
14	39.37039193153295	40.0	39.0	41.0	37.0	41.0
15	39.343318111712584	40.0	39.0	41.0	37.0	41.0
16	39.23596032298062	40.0	39.0	41.0	36.0	41.0
17	39.32745637644782	40.0	39.0	41.0	37.0	41.0
18	39.38852934950253	40.0	39.0	41.0	37.0	41.0
19	39.42042670940817	40.0	39.0	41.0	37.0	41.0
20	39.396195602866904	41.0	39.0	41.0	37.0	41.0
21	39.37712069434784	41.0	39.0	41.0	36.0	41.0
22	39.27586415459522	41.0	39.0	41.0	36.0	41.0
23	39.176505277164544	40.0	39.0	41.0	35.0	41.0
24	39.096879063719115	40.0	39.0	41.0	35.0	41.0
25	39.06275894396226	40.0	39.0	41.0	35.0	41.0
26	38.82628451326096	40.0	38.0	41.0	35.0	41.0
27	38.67406326549128	40.0	38.0	41.0	35.0	41.0
28	38.49981098980857	40.0	38.0	41.0	35.0	41.0
29	38.27336165966069	40.0	37.0	41.0	35.0	41.0
30	38.008452535760725	40.0	37.0	41.0	35.0	41.0
31	37.68268213022046	40.0	35.0	41.0	34.0	41.0
32	37.39275561738289	39.0	35.0	41.0	33.0	41.0
33	37.09786191671455	39.0	35.0	41.0	33.0	41.0
34	36.89372334956301	39.0	35.0	41.0	33.0	41.0
35	36.65135936129676	39.0	35.0	41.0	33.0	41.0
36	36.39085795506094	39.0	35.0	41.0	32.0	41.0
37	36.10035685124142	39.0	35.0	41.0	31.0	41.0
38	35.77769377324825	38.0	35.0	41.0	30.0	41.0
39	35.40112498865939	38.0	35.0	41.0	28.0	41.0
40	35.04521123778994	38.0	35.0	40.0	25.0	41.0
41	34.62585810626909	37.0	35.0	40.0	22.0	41.0
42	34.19428735597423	37.0	35.0	40.0	18.0	41.0
43	33.06773369220068	35.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	2.0
12	0.0
13	0.0
14	2.0
15	5.0
16	1.0
17	3.0
18	5.0
19	12.0
20	18.0
21	67.0
22	82.0
23	124.0
24	236.0
25	361.0
26	603.0
27	866.0
28	1166.0
29	1615.0
30	2040.0
31	2433.0
32	2815.0
33	3625.0
34	4783.0
35	6566.0
36	10403.0
37	24207.0
38	31522.0
39	38702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.623491698672396	16.225390873075877	13.378897390147277	20.772220038104454
2	20.850848277739136	22.962470136389754	30.91223878791545	25.274442797955665
3	23.149212205522122	24.842743520730636	28.806665255390573	23.20137901835667
4	15.102670335984516	17.163637463332023	29.11966613239786	38.614026068285604
5	14.797985907400127	38.379653430912995	28.41579217951432	18.40656848217256
6	30.599237910908155	30.262799770163607	18.396739952218226	20.741222366710012
7	28.11791211782139	32.81594943599359	18.380863096138146	20.685275350046876
8	33.837360510478725	28.072549671878306	18.098859890525297	19.991229927117672
9	30.425348534792995	11.900081652402697	18.703692503099766	38.97087730970454
10	22.47331176097015	26.72301690507152	24.15399038316146	26.649680950796867
11	33.872894426467475	24.302174373242206	16.955726252759547	24.869204947530772
12	22.954909728732574	30.485831796050444	24.448846281791514	22.110412193425468
13	34.70605135028881	18.33096440560075	24.49798893156319	22.464995312547252
14	24.32787975927662	22.092267215048235	25.93446638642756	27.645386639247587
15	30.74288565639459	23.995221822360662	22.68349109383978	22.578401427404966
16	23.032025886835818	24.488160401608855	27.261317930262802	25.21849578129253
17	21.448120482656424	28.849759579036498	25.664559833066196	24.037560105240875
18	21.334714367798714	22.059757462122356	30.271116218586506	26.334411951492427
19	22.573865182810657	26.284513260955027	31.89433574258324	19.24728581365107
20	23.677684700759066	22.60032660961079	32.59669761393534	21.1252910756948
21	26.46747512625881	22.04690476910515	30.311186379169563	21.174433725466475
22	25.674388363020533	23.222548159796776	28.777935706293285	22.325127770889406
23	22.949617443372546	24.78150421870747	29.782713883932622	22.48616445398736
24	22.080926603562464	25.67665648531769	30.049596274231106	22.19282063688874
25	21.76565760425802	24.97580669549702	29.541536879668552	23.716998820576404
26	23.374512353706113	23.803187467868266	29.89687603955605	22.92542413886957
27	22.765899537303053	24.445822118728643	29.042549974294612	23.745728369673692
28	21.653763570931744	25.523180209876916	30.65064868297699	22.172407536214354
29	21.92820636888741	25.49520670154535	31.452807935403875	21.123778994163363
30	21.15780082862068	26.031995645205193	32.43641697160311	20.373786554571023
31	21.50860374391387	26.766111228717453	30.655940968337013	21.069344059031664
32	20.30423080412496	27.496446608401126	31.325793086763237	20.873529500710678
33	20.613451477303656	26.425136843378596	31.52160764508422	21.439804034233525
34	20.779780445761638	26.790304533220432	29.65343091299483	22.776484108023105
35	19.759125412042216	27.381528412011974	30.6846705174343	22.174675658511507
36	19.889920464511444	26.939244564066893	30.21819336498624	22.95264160643542
37	19.482414491789395	27.341458251428918	30.125200350802917	23.05092690597877
38	20.29062207034203	25.635830283968915	31.722714488765234	22.350833156923823
39	20.067590044455198	25.635830283968915	31.73859134484531	22.557988326730577
40	19.632866604167294	25.511083557625426	31.51480327819276	23.34124656001452
41	17.752593219826412	25.714458523603597	32.607282184655396	23.9256660719146
42	17.83273354099253	25.4596727855566	32.31167024525963	24.39592342819125
43	17.325430187195693	26.24595518190341	32.75697825626758	23.67163637463332
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	34.5
2	60.0
3	268.5
4	477.0
5	477.0
6	621.5
7	766.0
8	753.5
9	741.0
10	1136.5
11	1532.0
12	1532.0
13	2544.0
14	3556.0
15	5302.5
16	7049.0
17	6103.0
18	5157.0
19	5157.0
20	5582.5
21	6008.0
22	4065.0
23	2122.0
24	1938.5
25	1755.0
26	1755.0
27	1623.0
28	1491.0
29	1489.0
30	1487.0
31	1474.0
32	1461.0
33	1461.0
34	1421.5
35	1382.0
36	1441.0
37	1500.0
38	1801.5
39	2103.0
40	2103.0
41	2595.5
42	3088.0
43	3994.5
44	4901.0
45	5916.5
46	6932.0
47	6932.0
48	8676.5
49	10421.0
50	10133.5
51	9846.0
52	8570.5
53	7295.0
54	7295.0
55	6960.5
56	6626.0
57	6848.0
58	7070.0
59	6685.0
60	6300.0
61	6300.0
62	6152.0
63	6004.0
64	5896.5
65	5789.0
66	5554.0
67	5319.0
68	5319.0
69	4914.5
70	4510.0
71	4035.5
72	3561.0
73	3047.0
74	2533.0
75	2533.0
76	2091.0
77	1649.0
78	1319.5
79	990.0
80	712.0
81	434.0
82	434.0
83	334.0
84	234.0
85	157.0
86	80.0
87	52.5
88	25.0
89	25.0
90	13.5
91	2.0
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	132268.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.63797743974355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.32649366324684	55.952308948498505
2	6.063971031985516	7.596697613935344
3	1.5896197948098976	2.9871170653521637
4	0.7736873868436934	1.9384885233011764
5	0.41641520820760414	1.304170320863701
6	0.24622812311406156	0.9253938972389391
7	0.22812311406155703	1.0002419330450298
8	0.17260108630054316	0.8649106359814921
9	0.127942063971032	0.7212628904950555
>10	0.928183464091732	11.2816403060453
>50	0.08690404345202173	3.9450207155169807
>100	0.035003017501508756	4.112105724740678
>500	0.0	0.0
>1k	0.004828002414001207	7.370641424985635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3297	2.4926664045725344	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3073	2.3233132730516832	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2306	1.74343000574591	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1073	0.8112317416155078	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.28880757250430944	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	364	0.27519883872138384	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	338	0.2555417788127136	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	331	0.25024949345268693	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	304	0.22983639277829862	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	294	0.22227598512111774	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	255	0.1927903952581123	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	248	0.1874981098980857	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	205	0.15498835697220795	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	200	0.1512081531436175	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	187	0.14137962318928238	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	178	0.13457525629781958	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.11038195179484077	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	145	0.1096259110291227	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	141	0.10660174796625034	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	140	0.10584570720053225	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	139	0.10508966643481416	No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA	138	0.10433362566909607	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	137	0.103577584903378	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	7.560407657180875E-4	7.560407657180875E-4
15	0.0	0.0	0.0	0.0052922853600266126	7.560407657180875E-4
16	0.0	0.0	0.0	0.007560407657180876	7.560407657180875E-4
17	0.0	0.0	0.0	0.010584570720053225	7.560407657180875E-4
18	0.0	0.0	0.0	0.012852693017207487	7.560407657180875E-4
19	0.0	0.0	0.0	0.016632896845797926	7.560407657180875E-4
20	0.0	0.0	0.0	0.020413100674388365	7.560407657180875E-4
21	0.0	0.0	0.0	0.028729549097287324	7.560407657180875E-4
22	0.0	0.0	0.0	0.047630568240239514	7.560407657180875E-4
23	0.0	0.0	0.0	0.060483261257447005	7.560407657180875E-4
24	0.0	7.560407657180875E-4	0.0	0.08316448422898963	7.560407657180875E-4
25	0.0	7.560407657180875E-4	0.0	0.09904134030906947	7.560407657180875E-4
26	0.0	7.560407657180875E-4	0.0	0.1217225632806121	7.560407657180875E-4
27	0.0	7.560407657180875E-4	0.0	0.2124474551667826	7.560407657180875E-4
28	0.0	7.560407657180875E-4	0.0	0.4180905434421024	7.560407657180875E-4
29	0.0	7.560407657180875E-4	0.0	0.6706081591919436	7.560407657180875E-4
30	0.0	7.560407657180875E-4	0.0	1.0879426618683279	7.560407657180875E-4
31	0.0	7.560407657180875E-4	0.0	1.7199927420086492	7.560407657180875E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTATG	20	0.0018360531	37.0	1
TTCTCCT	20	0.0018360531	37.0	29
AGTTCGG	25	0.0054789907	29.6	10
TATTGTT	45	3.973106E-6	28.777777	13
GTTATTG	40	5.901073E-5	27.750002	11
TTTACCG	35	8.827784E-4	26.428572	30
GTTTACC	35	8.827784E-4	26.428572	29
TTACCGG	35	8.827784E-4	26.428572	31
ATTGTTA	50	9.011921E-6	25.900002	14
TTATTGT	45	1.3145823E-4	24.666666	12
ACCGGGA	40	0.0019222131	23.125002	33
CCGGGAC	40	0.0019222131	23.125002	34
CGGGACT	40	0.0019222131	23.125002	35
CGAGACT	40	0.0019222131	23.125002	10
TACCGGG	40	0.0019222131	23.125002	32
CGTTATT	50	2.6846223E-4	22.2	10
TACCTTA	85	5.093898E-8	21.764706	31
GCTACCT	85	5.093898E-8	21.764706	29
TTGTGAT	60	3.6942914E-5	21.583334	34
TGTTACG	60	3.6942914E-5	21.583334	16
>>END_MODULE