FastQCFastQC Report
Fri 10 Feb 2017
ERR1631291.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631291.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321629
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.36097491208815125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10230.3180683333903348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7900.24562461718315198No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6230.19370143861405534No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT4620.14364376346660282No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC4600.14302192899272143No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4530.14084550833413653No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC4300.13369441188450046No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG4090.1271651499087458No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.12032497069605043No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT3670.11410662595723645No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC3620.11255203977253295No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG3360.10446819161207478No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA3280.10198085371654919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA250.00549053129.64
CGGTGAT1050.026.4285714
TCGGTGA1100.025.22727213
AGTCGGT1100.025.22727211
GATTCCT1250.023.68000218
ATACACA1350.023.29629737
GCAGTCG1200.023.1250029
CTGAGTA400.001928180923.1254
TAGAATC400.001928180923.1259
GCCTTAT1005.293259E-1022.226
CGCCTTA1005.293259E-1022.225
TCTAATA502.696242E-422.22
CAGTCGG1250.022.210
GGTATCA6900.021.9855081
GTCGGTG1350.021.92592612
GGGTATC450.003819909620.5555551
ATAGAAT450.003819909620.5555558
TATTCAA450.003819909620.5555555
ATTCCTC1650.020.1818219
TCGCCTT1202.3646862E-1020.04166824
Produced by FastQC (version 0.11.5)