##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631291.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321629
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85206557866361	34.0	33.0	34.0	31.0	34.0
2	33.02096204011454	34.0	33.0	34.0	31.0	34.0
3	33.12719624163244	34.0	33.0	34.0	31.0	34.0
4	36.446197326733596	37.0	37.0	37.0	35.0	37.0
5	36.42980577000208	37.0	37.0	37.0	35.0	37.0
6	36.471176417549415	37.0	37.0	37.0	35.0	37.0
7	36.4691150362685	37.0	37.0	37.0	35.0	37.0
8	36.46361491034701	37.0	37.0	37.0	35.0	37.0
9	38.30846409994124	39.0	39.0	39.0	37.0	39.0
10	38.286721657561976	39.0	39.0	39.0	37.0	39.0
11	38.333906457440094	39.0	39.0	39.0	37.0	39.0
12	38.124149252710424	39.0	39.0	39.0	37.0	39.0
13	38.24971006967655	39.0	39.0	39.0	37.0	39.0
14	39.779503713906394	41.0	40.0	41.0	38.0	41.0
15	39.77028501783111	41.0	40.0	41.0	38.0	41.0
16	39.73783769498397	41.0	40.0	41.0	38.0	41.0
17	39.75207148609112	41.0	40.0	41.0	38.0	41.0
18	39.73276663484947	41.0	40.0	41.0	38.0	41.0
19	39.738077101256415	41.0	40.0	41.0	38.0	41.0
20	39.70525045938022	41.0	40.0	41.0	38.0	41.0
21	39.67397840368872	41.0	40.0	41.0	37.0	41.0
22	39.60892208103125	41.0	40.0	41.0	37.0	41.0
23	39.54395903354486	41.0	40.0	41.0	37.0	41.0
24	39.514048795351165	41.0	39.0	41.0	37.0	41.0
25	39.508971516871924	41.0	39.0	41.0	37.0	41.0
26	39.432743937891175	41.0	39.0	41.0	36.0	41.0
27	39.359075207770445	41.0	39.0	41.0	36.0	41.0
28	39.26059528214185	41.0	39.0	41.0	36.0	41.0
29	39.19025958480112	41.0	39.0	41.0	35.0	41.0
30	39.134468595804485	41.0	39.0	41.0	35.0	41.0
31	39.07734688103374	41.0	39.0	41.0	35.0	41.0
32	38.9944718915272	40.0	39.0	41.0	35.0	41.0
33	38.87555537591449	40.0	38.0	41.0	35.0	41.0
34	38.84029736124541	40.0	38.0	41.0	35.0	41.0
35	38.7272043254806	40.0	38.0	41.0	35.0	41.0
36	38.66429022258565	40.0	38.0	41.0	35.0	41.0
37	38.58790407581406	40.0	38.0	41.0	35.0	41.0
38	38.48830484813247	40.0	38.0	41.0	35.0	41.0
39	38.39017314980925	40.0	38.0	41.0	35.0	41.0
40	38.270028511110624	40.0	38.0	41.0	35.0	41.0
41	38.15991717164808	40.0	37.0	41.0	34.0	41.0
42	38.02253217216109	40.0	37.0	41.0	34.0	41.0
43	37.12085974834359	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	5.0
19	13.0
20	25.0
21	52.0
22	92.0
23	161.0
24	197.0
25	315.0
26	556.0
27	685.0
28	1030.0
29	1505.0
30	2055.0
31	2680.0
32	3452.0
33	4532.0
34	6743.0
35	9857.0
36	15947.0
37	30762.0
38	64994.0
39	175968.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.149094142630176	18.37738512385388	12.881922960927032	25.591597772588916
2	19.731118773493684	19.97052504593802	33.28835397305591	27.010002207512386
3	20.45462318385469	21.475053555494064	28.65009063237457	29.420232628276676
4	14.786291037188812	15.741428789070639	32.90188384753862	36.57039632620192
5	16.590232845918745	34.841385571574705	31.492806929723375	17.075574652783175
6	34.4807215767235	33.20782640868828	16.077841239440474	16.233610775147763
7	30.26654934722926	29.81198834682196	20.075304154787037	19.84615815116174
8	28.852186836386025	30.727950526849256	19.433570977741436	20.986291659023284
9	27.278634700229148	14.186531687130202	19.814755510230732	38.72007810240992
10	19.927929384477146	24.547848608178988	29.204456065839835	26.31976594150403
11	37.55289479493454	21.10972580208874	19.830301372077766	21.507078030898956
12	23.754076902269386	25.209169571151858	26.330958962033897	24.705794564544863
13	30.90766068980098	17.914118440812242	24.755852239692317	26.42236862969446
14	24.293829225598436	20.26403091761004	23.25318923355792	32.1889506232336
15	27.375329960917703	25.95537093980953	20.78823737909206	25.881061720180703
16	26.730798528739637	24.536966504886067	23.001657188872894	25.730577777501406
17	25.495213429137298	25.2393285431351	23.388438231627124	25.877019796100477
18	25.801466907523885	22.555490953862993	25.39914000292262	26.2439021356905
19	25.70694806749391	24.383995224311242	24.666308075453394	25.242748632741453
20	26.169281998824733	22.890970652522004	24.73470986758035	26.205037481072914
21	28.328913126614825	23.389992817811827	24.06748147710561	24.21361257846774
22	27.84574774040898	22.84961866000889	23.653650634737538	25.650982964844587
23	25.783122790544382	23.039900009016602	24.56494905621073	26.61202814422829
24	25.163464737321572	24.284501708490218	24.66008973071458	25.89194382347363
25	25.739594377372686	23.232979613156772	24.386482562206766	26.64094344726377
26	25.696065964200987	24.443691333803855	24.615939483069003	25.244303218926156
27	26.578759999875633	23.081562918766654	24.27206501901259	26.067612062345123
28	25.04065242873	23.523998146933266	25.459768864126058	25.975580560210677
29	24.73470986758035	23.750345895426097	25.623933165230746	25.891011071762808
30	24.074632573555245	24.68527402690678	25.83939881043065	25.400694589107324
31	25.392299823709923	23.968609795758468	24.354769004038815	26.28432137649279
32	24.87368987249284	23.312574425813594	24.70517273007098	27.108562971622586
33	24.084581925137346	22.964968954913893	25.84996999648663	27.100479123462122
34	25.73617428776634	22.730226441023664	25.559884214420965	25.973715056789032
35	25.405358347661437	22.57632240873802	26.409931940216836	25.60838730338371
36	23.482957071657097	23.478293313102984	26.24732222529685	26.791427389943074
37	24.58888968345516	22.226540517179732	26.86604752680884	26.318522272556265
38	23.48513349231568	21.401366170339116	28.01923955862189	27.09426077872331
39	24.125312083176578	21.381778384411852	27.991567924534166	26.5013416078774
40	23.16830882787311	22.01636046500782	28.55588270958154	26.25944799753753
41	22.14632387004903	22.39474674236465	29.72057867916139	25.738350708424925
42	23.020623140326276	22.347798239586606	29.19357396254691	25.43800465754021
43	21.12309524327719	21.786281709671705	29.396913835506126	27.69370921154498
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	55.0
2	56.0
3	140.5
4	225.0
5	225.0
6	290.0
7	355.0
8	326.0
9	297.0
10	464.5
11	632.0
12	632.0
13	994.0
14	1356.0
15	1922.5
16	2489.0
17	2216.0
18	1943.0
19	1943.0
20	2203.0
21	2463.0
22	1999.5
23	1536.0
24	1722.0
25	1908.0
26	1908.0
27	2172.5
28	2437.0
29	2967.0
30	3497.0
31	4145.5
32	4794.0
33	4794.0
34	5949.0
35	7104.0
36	8708.0
37	10312.0
38	11825.0
39	13338.0
40	13338.0
41	14752.5
42	16167.0
43	17436.5
44	18706.0
45	20275.0
46	21844.0
47	21844.0
48	23400.0
49	24956.0
50	25021.5
51	25087.0
52	25504.0
53	25921.0
54	25921.0
55	24759.0
56	23597.0
57	22848.0
58	22099.0
59	21307.5
60	20516.0
61	20516.0
62	19202.5
63	17889.0
64	16666.5
65	15444.0
66	13665.0
67	11886.0
68	11886.0
69	10101.0
70	8316.0
71	7057.5
72	5799.0
73	4352.5
74	2906.0
75	2906.0
76	2317.5
77	1729.0
78	1450.5
79	1172.0
80	1103.5
81	1035.0
82	1035.0
83	850.0
84	665.0
85	574.0
86	483.0
87	434.5
88	386.0
89	386.0
90	282.0
91	178.0
92	103.5
93	29.0
94	23.5
95	18.0
96	18.0
97	11.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	321629.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.74182183427179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.81880908203324	53.061974677110314
2	5.7139596289259185	6.827247162390279
3	1.8488564829468412	3.3136216380404564
4	0.9289773164064178	2.219951892993286
5	0.5337559850929543	1.5943777482199752
6	0.33485096356569577	1.2002763963825618
7	0.244070775753089	1.0206862959995482
8	0.17088306204676207	0.8167092357833975
9	0.1437170856739952	0.7727328474188935
>10	1.0246053923012128	12.086224655157546
>50	0.1360213624801969	5.66737430251672
>100	0.09940022642783616	10.294599612656247
>500	0.0010463181729245912	0.4416257590832254
>1k	0.0010463181729245912	0.682597776247533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1161	0.36097491208815125	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1023	0.3180683333903348	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	790	0.24562461718315198	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	623	0.19370143861405534	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	462	0.14364376346660282	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	460	0.14302192899272143	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	453	0.14084550833413653	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	430	0.13369441188450046	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	409	0.1271651499087458	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	387	0.12032497069605043	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	367	0.11410662595723645	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	362	0.11255203977253295	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	336	0.10446819161207478	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	328	0.10198085371654919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.1091723694069874E-4	0.0	0.0	0.0
3	0.0	3.1091723694069874E-4	0.0	0.0	0.0
4	0.0	3.1091723694069874E-4	0.0	0.0	0.0
5	0.0	3.1091723694069874E-4	0.0	0.0	0.0
6	0.0	3.1091723694069874E-4	0.0	0.0	0.0
7	0.0	3.1091723694069874E-4	0.0	0.0	0.0
8	0.0	3.1091723694069874E-4	0.0	0.0	0.0
9	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.0	0.0
10	3.1091723694069874E-4	3.1091723694069874E-4	0.0	3.1091723694069874E-4	0.0
11	3.1091723694069874E-4	3.1091723694069874E-4	0.0	3.1091723694069874E-4	0.0
12	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.001243668947762795	0.0
13	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.0015545861847034938	0.0
14	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.0018655034216441926	0.0
15	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.00248733789552559	0.0
16	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.004352841317169782	0.0
17	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.006218344738813975	0.0
18	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.006840179212695373	0.0
19	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.00746201368657677	0.0
20	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.008083848160458167	0.0
21	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.010260268819043059	0.0
22	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.01492402737315354	0.0
23	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.02238604105973031	0.0
24	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.03606639948512105	0.0
25	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.04383933040863852	0.0
26	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.060317943966495556	0.0
27	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.12219047411769461	0.0
28	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.35351289840157446	0.0
29	3.1091723694069874E-4	3.1091723694069874E-4	0.0	0.6756231558721384	0.0
30	3.1091723694069874E-4	3.1091723694069874E-4	0.0	1.1479064387850597	0.0
31	3.1091723694069874E-4	3.1091723694069874E-4	0.0	2.034331481302992	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACA	25	0.005490531	29.6	4
CGGTGAT	105	0.0	26.42857	14
TCGGTGA	110	0.0	25.227272	13
AGTCGGT	110	0.0	25.227272	11
GATTCCT	125	0.0	23.680002	18
ATACACA	135	0.0	23.296297	37
GCAGTCG	120	0.0	23.125002	9
CTGAGTA	40	0.0019281809	23.125	4
TAGAATC	40	0.0019281809	23.125	9
GCCTTAT	100	5.293259E-10	22.2	26
CGCCTTA	100	5.293259E-10	22.2	25
TCTAATA	50	2.696242E-4	22.2	2
CAGTCGG	125	0.0	22.2	10
GGTATCA	690	0.0	21.985508	1
GTCGGTG	135	0.0	21.925926	12
GGGTATC	45	0.0038199096	20.555555	1
ATAGAAT	45	0.0038199096	20.555555	8
TATTCAA	45	0.0038199096	20.555555	5
ATTCCTC	165	0.0	20.18182	19
TCGCCTT	120	2.3646862E-10	20.041668	24
>>END_MODULE