FastQCFastQC Report
Fri 10 Feb 2017
ERR1631290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247654
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA22350.17913620282546283No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18500.14827828869221754No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC16480.1320879025755538No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC16220.13000399149123074No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15390.12335150610666099No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14830.1188630822327344No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT14690.11774097626425274No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC13790.11052743789544217No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC13600.10900457979535992No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC13530.10844352681111911No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG13120.1051573593319943No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG12770.10235209441079017No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA12650.10139028929494877No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG12500.10018803290014698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAG251.2336862E-437.02
TCTAGAC2700.023.981483
GTACTAT701.9231993E-723.7857131
TCTAATA551.9028277E-523.5454542
GTTTCGA400.001931279323.1252
TAGGAGT400.001931279323.1256
TATCGCC400.001931279323.12533
GGTATCA6550.022.0305331
CTAGACA2950.021.9491544
TAGAACT2850.021.4210534
GACATGT2100.020.2619047
GTCTAGA2750.020.1818181
ACATGTA1850.020.08
CTTATAC14600.019.76712237
TATACAC3650.019.76712237
CCGACGA759.266814E-619.73333436
ACTGATC2450.019.6326548
TTACACT2650.019.547174
TACTATA951.6763988E-719.4736842
GTTCTAG3500.019.0285721
Produced by FastQC (version 0.11.5)