##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631290.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1247654
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.877343398089536	34.0	33.0	34.0	31.0	34.0
2	33.046140195919705	34.0	33.0	34.0	31.0	34.0
3	33.16402864896838	34.0	33.0	34.0	31.0	34.0
4	36.48721360248915	37.0	37.0	37.0	35.0	37.0
5	36.45930442254022	37.0	37.0	37.0	35.0	37.0
6	36.500153087314274	37.0	37.0	37.0	35.0	37.0
7	36.48966299951749	37.0	37.0	37.0	35.0	37.0
8	36.468078489709484	37.0	37.0	37.0	35.0	37.0
9	38.33247679244406	39.0	39.0	39.0	37.0	39.0
10	38.29042587127521	39.0	39.0	39.0	37.0	39.0
11	38.35045132705061	39.0	39.0	39.0	37.0	39.0
12	38.12813247903666	39.0	39.0	39.0	37.0	39.0
13	38.25484469251892	39.0	39.0	39.0	37.0	39.0
14	39.798847276568665	41.0	40.0	41.0	38.0	41.0
15	39.8066178604004	41.0	40.0	41.0	38.0	41.0
16	39.80425983485806	41.0	40.0	41.0	38.0	41.0
17	39.79487822745729	41.0	40.0	41.0	38.0	41.0
18	39.77536881218671	41.0	40.0	41.0	38.0	41.0
19	39.77445189130961	41.0	40.0	41.0	38.0	41.0
20	39.743474553041146	41.0	40.0	41.0	38.0	41.0
21	39.708303744467614	41.0	40.0	41.0	38.0	41.0
22	39.66962875925537	41.0	40.0	41.0	37.0	41.0
23	39.61536050860254	41.0	40.0	41.0	37.0	41.0
24	39.575336591715335	41.0	40.0	41.0	37.0	41.0
25	39.56040055977058	41.0	40.0	41.0	37.0	41.0
26	39.510823513570266	41.0	39.0	41.0	37.0	41.0
27	39.446687142428914	41.0	39.0	41.0	37.0	41.0
28	39.35848480428067	41.0	39.0	41.0	36.0	41.0
29	39.30861440752003	41.0	39.0	41.0	36.0	41.0
30	39.26259924626539	41.0	39.0	41.0	36.0	41.0
31	39.23507078084148	41.0	39.0	41.0	36.0	41.0
32	39.165101061672544	41.0	39.0	41.0	35.0	41.0
33	39.07739485466323	40.0	39.0	41.0	35.0	41.0
34	39.03955503689324	40.0	39.0	41.0	35.0	41.0
35	38.94058208445611	40.0	39.0	41.0	35.0	41.0
36	38.89751325287299	40.0	39.0	41.0	35.0	41.0
37	38.83851692857154	40.0	38.0	41.0	35.0	41.0
38	38.74575803868701	40.0	38.0	41.0	35.0	41.0
39	38.69622507522117	40.0	38.0	41.0	35.0	41.0
40	38.60458268077528	40.0	38.0	41.0	35.0	41.0
41	38.52876598800629	40.0	38.0	41.0	35.0	41.0
42	38.41871464364319	40.0	38.0	41.0	35.0	41.0
43	37.48348420315248	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	2.0
16	4.0
17	3.0
18	21.0
19	29.0
20	80.0
21	137.0
22	260.0
23	462.0
24	737.0
25	1056.0
26	1587.0
27	2355.0
28	3338.0
29	4719.0
30	6170.0
31	8428.0
32	11270.0
33	15647.0
34	23123.0
35	34641.0
36	58450.0
37	109822.0
38	266751.0
39	698560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66466985237894	17.104341427992058	11.306580189700028	27.924408529928968
2	20.20736518297541	18.92095084053752	32.747139832036765	28.124544144450304
3	20.981858752506703	19.601347809569	27.357264113287815	32.05952932463648
4	15.402266974658039	14.059426732090788	31.66583043055206	38.87247586269911
5	17.581717367154674	33.38489677426594	31.47467166377858	17.558714194800803
6	37.93760129010126	32.02354178321875	14.431164409363491	15.607692517316499
7	32.88740307809697	27.46690989649374	18.98963975589386	20.656047269515426
8	28.98976799657597	30.850379993171185	18.650122550001843	21.509729460251
9	27.982998491568978	13.373258932364262	17.926925253315424	40.71681732275134
10	18.897306464773088	24.0328648808083	29.501768919908884	27.568059734509724
11	40.16586329222685	19.511018279106228	19.13070450621727	21.192413922449653
12	25.28569619461806	23.00990498968464	25.537929586247472	26.16646922944983
13	32.44200715903608	16.504255186133335	23.144718006755078	27.909019648075507
14	24.969823364490477	18.46208964985485	21.552529787905943	35.015557197748734
15	27.701029291774802	25.019276177529985	19.21790817005356	28.061786360641648
16	28.120536623134296	23.201063756458122	20.967511826195402	27.71088779421218
17	27.25715623081399	23.546351793045186	21.91873708576256	27.277754890378265
18	28.14899002447794	21.294204963876204	23.05062140625526	27.5061836053906
19	27.617833149254523	22.549440790475565	22.29239837326695	27.540327687002968
20	27.789755813711174	21.705937703882647	22.17802371490814	28.32628276749804
21	29.335136183589363	21.92651167711561	22.49397669546204	26.244375443832986
22	29.100616036176696	21.727898920694358	21.486165234912885	27.685319808216057
23	27.3452415493398	21.759638489517126	22.596809692430753	28.298310268712317
24	27.300758062732132	22.588153446388183	22.10212126118299	28.008967229696697
25	27.837204866092684	21.780718051639315	22.0842477161136	28.2978293661544
26	27.667526413572997	22.923582980537873	22.19597741040385	27.21291319548529
27	28.268013407563313	21.54419414356865	22.3129168824049	27.874875566463135
28	27.050368130908087	21.98806720452946	23.120833179711685	27.840731484850767
29	26.696343697852125	22.501590985962455	22.794620944588804	28.007444371596613
30	25.931628480331888	22.983455348999	23.701362717548292	27.38355345312082
31	27.874394663905218	22.33680170944829	21.892527896355883	27.89627573029061
32	26.8539995864238	21.860868477959432	22.41566972894729	28.86946220666948
33	26.645207725859894	21.616089075977797	22.956204204050163	28.78249899411215
34	27.922084167565686	21.50435938168755	23.112577685800712	27.460978764946052
35	27.22621816625443	21.425811963893835	24.206230252938717	27.141739616913025
36	26.188751047966825	21.62690938353101	24.304093923475577	27.880245645026587
37	27.078420780120126	20.64979553626246	24.68793431512262	27.58384936849479
38	25.702157809777393	19.89341596307951	25.951505786059275	28.45292044108383
39	26.446033916454404	19.748343691440095	25.712978117330604	28.092644274774898
40	25.30597425247705	20.902830432155067	26.032217265363634	27.758978050004245
41	24.77489752767995	20.694198872443803	27.385637364205138	27.145266235671105
42	25.518292731799043	20.448938567904243	27.244973366013337	26.78779533428338
43	23.516936586585704	19.933170574534287	27.337146356281465	29.21274648259854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	19.5
2	21.0
3	53.0
4	85.0
5	85.0
6	90.5
7	96.0
8	99.0
9	102.0
10	152.5
11	203.0
12	203.0
13	310.5
14	418.0
15	663.5
16	909.0
17	874.0
18	839.0
19	839.0
20	1045.0
21	1251.0
22	1310.5
23	1370.0
24	1721.5
25	2073.0
26	2073.0
27	2720.0
28	3367.0
29	4495.0
30	5623.0
31	7500.0
32	9377.0
33	9377.0
34	13218.0
35	17059.0
36	22534.0
37	28009.0
38	34443.5
39	40878.0
40	40878.0
41	45493.5
42	50109.0
43	55437.0
44	60765.0
45	68182.5
46	75600.0
47	75600.0
48	82599.5
49	89599.0
50	93814.0
51	98029.0
52	103949.0
53	109869.0
54	109869.0
55	110042.5
56	110216.0
57	109209.0
58	108202.0
59	104729.0
60	101256.0
61	101256.0
62	97162.5
63	93069.0
64	87107.5
65	81146.0
66	71120.0
67	61094.0
68	61094.0
69	50305.0
70	39516.0
71	33564.0
72	27612.0
73	19956.5
74	12301.0
75	12301.0
76	8935.0
77	5569.0
78	4475.0
79	3381.0
80	3183.5
81	2986.0
82	2986.0
83	2461.5
84	1937.0
85	1825.0
86	1713.0
87	1467.0
88	1221.0
89	1221.0
90	919.0
91	617.0
92	354.0
93	91.0
94	65.0
95	39.0
96	39.0
97	27.5
98	16.0
99	9.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1247654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.80792170919873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89110188695172	26.538689376779296
2	7.784719840724427	5.10800958125066
3	3.0493165237489315	3.0012521333316298
4	1.698244257221208	2.22863458536039
5	1.0706763689277599	1.756333324383556
6	0.7398044537455236	1.4562867959159802
7	0.5700744558364517	1.3092070680847463
8	0.4332873566355895	1.137220613926887
9	0.34084040523041137	1.0064038797117816
>10	2.6532111367073306	17.94544811690771
>50	0.41599348534518704	9.540012909521248
>100	0.3219613511810534	20.75872965337316
>500	0.02535322566141011	5.818791722729399
>1k	0.005415252083019635	2.394980238723538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2235	0.17913620282546283	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1850	0.14827828869221754	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1648	0.1320879025755538	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1622	0.13000399149123074	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1539	0.12335150610666099	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1483	0.1188630822327344	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1469	0.11774097626425274	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1379	0.11052743789544217	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1360	0.10900457979535992	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1353	0.10844352681111911	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1312	0.1051573593319943	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1277	0.10235209441079017	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1265	0.10139028929494877	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	1250	0.10018803290014698	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	8.015042632011759E-5	0.0	0.0	0.0
11	0.0	8.015042632011759E-5	0.0	0.0	0.0
12	0.0	8.015042632011759E-5	0.0	0.0	0.0
13	0.0	8.015042632011759E-5	0.0	0.0	0.0
14	0.0	8.015042632011759E-5	0.0	0.0	0.0
15	0.0	8.015042632011759E-5	0.0	8.015042632011759E-5	0.0
16	0.0	8.015042632011759E-5	0.0	5.610529842408232E-4	0.0
17	0.0	8.015042632011759E-5	0.0	9.618051158414112E-4	0.0
18	0.0	8.015042632011759E-5	0.0	0.001362557247441999	0.0
19	0.0	8.015042632011759E-5	0.0	0.001362557247441999	0.0
20	0.0	8.015042632011759E-5	0.0	0.0016831589527224696	0.0
21	0.0	8.015042632011759E-5	0.0	0.002404512789603528	0.0
22	0.0	8.015042632011759E-5	0.0	0.0032861674791248214	0.0
23	0.0	8.015042632011759E-5	0.0	0.004568574300246703	0.0
24	0.0	8.015042632011759E-5	0.0	0.006492184531929525	0.0
25	0.0	8.015042632011759E-5	0.0	0.007854741779371524	0.0
26	0.0	8.015042632011759E-5	0.0	0.01202256394801764	0.0
27	0.0	8.015042632011759E-5	0.0	0.03366317905444939	0.0
28	0.0	8.015042632011759E-5	0.0	0.1361755743178798	0.0
29	0.0	1.6030085264023518E-4	0.0	0.29278950734738957	0.0
30	0.0	1.6030085264023518E-4	0.0	0.5018218191902563	0.0
31	0.0	1.6030085264023518E-4	0.0	1.1225067206132469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAAG	25	1.2336862E-4	37.0	2
TCTAGAC	270	0.0	23.98148	3
GTACTAT	70	1.9231993E-7	23.785713	1
TCTAATA	55	1.9028277E-5	23.545454	2
GTTTCGA	40	0.0019312793	23.125	2
TAGGAGT	40	0.0019312793	23.125	6
TATCGCC	40	0.0019312793	23.125	33
GGTATCA	655	0.0	22.030533	1
CTAGACA	295	0.0	21.949154	4
TAGAACT	285	0.0	21.421053	4
GACATGT	210	0.0	20.261904	7
GTCTAGA	275	0.0	20.181818	1
ACATGTA	185	0.0	20.0	8
CTTATAC	1460	0.0	19.767122	37
TATACAC	365	0.0	19.767122	37
CCGACGA	75	9.266814E-6	19.733334	36
ACTGATC	245	0.0	19.632654	8
TTACACT	265	0.0	19.54717	4
TACTATA	95	1.6763988E-7	19.473684	2
GTTCTAG	350	0.0	19.028572	1
>>END_MODULE