FastQCFastQC Report
Fri 10 Feb 2017
ERR1631289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG21.7857142857142856No Hit
CATCTTTGATGCCAAGAATGCATGTGATCACCTATCGGGATTC10.8928571428571428No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAG10.8928571428571428No Hit
GTCCCTTCAGCACCTGACCCTATGCCCACCGGTTATTCCTAGG10.8928571428571428No Hit
TACTTAGACATGCATGGCTTAATCTTTGAGCTGTCTCTTATAC10.8928571428571428No Hit
CACCTGAGGAGCTGGGCCTTCTGGGGCCCCCCAGGCCACAGGT10.8928571428571428No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC10.8928571428571428No Hit
CCCCTACACACCGTCCGTTCACCCCCGTCTCTTCCTTCTGCTT10.8928571428571428No Hit
TGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAA10.8928571428571428No Hit
AAGCTGGGAGGGAGCGTGCCAACCCCTTTTGCCTCTGGGTTTG10.8928571428571428No Hit
ACGAATATCTGGCCGAGGCCGCGCCCTGGACCGCCATCAAGAC10.8928571428571428No Hit
ACCTTGAATGTATAATTTAAAAATTATATTTTTCACAACTAAG10.8928571428571428No Hit
ATTGTGGGAGCAGACAATGTGGGCTCCAAGCCTGTCTCTTATA10.8928571428571428No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGT10.8928571428571428No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCA10.8928571428571428No Hit
GTCGAAGGGGGTTCGGTTGGTCTCTGCTAGTGTGGAGATAAAT10.8928571428571428No Hit
GTAGAAGACGTAATCCCGCTGTTCCTCCTTGCTCCCTTTGGGC10.8928571428571428No Hit
CGGATGTTCTTTGTACTGTTCTTTGGTCTGTTGCGCAGGTTCG10.8928571428571428No Hit
CCCTAGCGTGGACAAGACCGGAGAGAACCAAAAGCACCTCCTG10.8928571428571428No Hit
CATCAATATCCTGCACCGCTACTATAAATCAGCCTTGGTACAG10.8928571428571428No Hit
TTTTGTCACTTTCGGAGAAAGTGGAATGCCAAAAAAAAAAAAA10.8928571428571428No Hit
AGCCTGACCGGCCGCGGCAAGGGGATAAAGATAATAACGTCCG10.8928571428571428No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCC10.8928571428571428No Hit
GTCAGCTGAGAGCGCAGGTCATGGAGGCCGATGTCTGCCACAT10.8928571428571428No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACA10.8928571428571428No Hit
ATGAGCCCCCTTCTTCTTCTTCTTCCTCATCATCATCTTCATC10.8928571428571428No Hit
ACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTT10.8928571428571428No Hit
GCTCTTTCTTCCTGTCTGCTTGGAAAGATGGCGTCCCGCAAGG10.8928571428571428No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT10.8928571428571428No Hit
CCTTCGAGGACTTCCTGAAGATCTGGCAGGGGATCGACATTGA10.8928571428571428No Hit
AGCCATGATCCTGCAACTCAATCCCAGTGAGAACTGCACCTGG10.8928571428571428No Hit
CAACAAGCACAGGGTAGAACCTGTCACAGGAATAAAGGTGCAT10.8928571428571428No Hit
GACAAGAGGATTGCTTGAGCCCAGGAAGGAGTTGGAGACCAGC10.8928571428571428No Hit
CGGTACAGGTTCCATCAGATGTGGTATCCCAAAAACAGGAGCT10.8928571428571428No Hit
AGCCAAGCCCACTTCCAGGAACGGTGTCCCAAATCCTGTCTCT10.8928571428571428No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC10.8928571428571428No Hit
GGTATGAAGCATTACCACTTAAAGAGGAACCAGAGCTTCTGCC10.8928571428571428No Hit
CAAGCAGACACATCCAGGCATTTGGGCAAGAAACTTCTTCCTT10.8928571428571428No Hit
ACTCCAGTGCCAGCGGCGGTTCTTTTTTGGGTCATGGAGGCCA10.8928571428571428No Hit
ACTGTGAGCTGGGAACTAGCAAGAATCAAAAAGCCAGTGTATG10.8928571428571428No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10.8928571428571428No Hit
GTCCTATTCCAGTATTCCTAGCTGCGGTATCCAGGCGGCTCGG10.8928571428571428No Hit
GTCAAAACTTACTAAGTTGCCAGCATTCATGCACAACTAGAAA10.8928571428571428No Hit
AATCTAGAATCCCATAGTGCTGAAGGTAGAGGTGTCTGTGCAA10.8928571428571428No Hit
GAAGCCTGTGGCTACAAAAAATGTTGAGCCGTAGATGCCGTCG10.8928571428571428No Hit
CTCCTCTTCTCCATGCTGGCACTTGAACTCCCACCTGCCACTC10.8928571428571428No Hit
GGCTGGAGCGGCGCCATAAATGCAGCATTTGTGGCAAGATGTT10.8928571428571428No Hit
AAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAG10.8928571428571428No Hit
CTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGA10.8928571428571428No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCG10.8928571428571428No Hit
GACATGTAGGGCTTCATCACCATGAACATCTTGAACAGGTTGC10.8928571428571428No Hit
ACCTTGAGGGCAGCAGGAACCACCATCCGCTTTTTCTTGTCGT10.8928571428571428No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTGTCTCTT10.8928571428571428No Hit
TAAAGGAACACGCCAAGAAACACACACATTTTATTTACCCCTT10.8928571428571428No Hit
GAGTCCTGGAGCCACAGTCTTTTTTGCTTTGCATTGTAGGAGA10.8928571428571428No Hit
CTACAAAAATATTTTTTTTAAAAAATTTCTTTTAAAAATTTTC10.8928571428571428No Hit
CCCGCCACCGTGCCAGGCCAATGTTTGTATTTTTAGTAGAGAC10.8928571428571428No Hit
GATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT10.8928571428571428No Hit
TCTTTACAGATTGCTCCCGTCTCTCAATCTGAAGCACTCCTCT10.8928571428571428No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGG10.8928571428571428No Hit
ACTATGCTCCTTGCACAGAATGGGAAGCCAGGAAAATGCAAAT10.8928571428571428No Hit
TTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACC10.8928571428571428No Hit
TTTCTGCAGAAGGCGCTGGATGCGCACGAGGCGAAGCGCTTCG10.8928571428571428No Hit
GTACATGGGGGCTTCTCCTAACTTTAGTTTTGGCGTTTGAGGC10.8928571428571428No Hit
ACATGGGCAACGACCGTACCGCAAACCGACACTGGTGCGCGAG10.8928571428571428No Hit
GGGGTCGCGTAACTAGTTGGCATGCCAGAGTCTCGTTCGTTAT10.8928571428571428No Hit
CAATAATAGAGACATGTATCCCACTTTCAGTATTGGATAAAAT10.8928571428571428No Hit
CAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGG10.8928571428571428No Hit
TACGTACTCAGTAGTGCCCTGCTTCTAGGGCTCTGAATACGGG10.8928571428571428No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA10.8928571428571428No Hit
TATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCTGTCTCTT10.8928571428571428No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT10.8928571428571428RNA PCR Primer, Index 29 (95% over 23bp)
GTACATGGGCCGGCATCTCGGCCAAGGCGAAGTTCGTGCTCGG10.8928571428571428No Hit
CATCTGGATGATGGACCCCAAGGATGTCCGTGAGTGGCAACAT10.8928571428571428No Hit
CCAGTGGCTCACTCGCTCCTGGCACCTTCCCTTTCAGGCTCCA10.8928571428571428No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.8928571428571428No Hit
GCCTATTTCTCATAAGTCACATTTTGTTGGCTTCTGGAAAAGC10.8928571428571428No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTT10.8928571428571428No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAGATAA10.8928571428571428No Hit
CTCGAGAGGGGGCAGGGAGGGAGAAAAGTGCAAGGTACCGCGG10.8928571428571428No Hit
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAC10.8928571428571428No Hit
AACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGT10.8928571428571428No Hit
GTACTCTTCATGGACCACCATTCGCTGTTGGCAAGAAACGGCT10.8928571428571428No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAAT10.8928571428571428No Hit
TCACAAATACTTGCTGTCCAGCACACCTACCCTCCCATATTTC10.8928571428571428No Hit
CAGGTGATCTGCCCACCTTGGCCTCCCCCAAAGGCGGGGGTGA10.8928571428571428No Hit
GATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGC10.8928571428571428No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGC10.8928571428571428No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGGCATGATTACTGT10.8928571428571428No Hit
GAGCAGGGCTGCGGTCACCTCCCACATCTGAAGAGAACCAACC10.8928571428571428No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGA10.8928571428571428No Hit
GCAGACAGAACTGTTTCAAGTCTGCAGCTGCCTGGGAAACTTT10.8928571428571428No Hit
GCCATACAGCCTCTTGGCATCCTCCGTGAACCTGTCCAGCAGA10.8928571428571428No Hit
GATTTAAGCGAGAGCCTCACTGGTTCCCAGGACATCAACCTGA10.8928571428571428No Hit
ACATAATACAGGGCTGACCTGCGATTGGTGCAAAAGCGGATGC10.8928571428571428No Hit
CAATTGTAGTTAACAAGTGCTATTTCTCTGGTTGTTTTTTGTT10.8928571428571428No Hit
AAGCCAAACCTCTTCTGAACTACAGCAGTCAGTTCCCGAATCC10.8928571428571428No Hit
AGTCTTAGCAGCCAAGATGAATTTGTTAAACAATCAGATAGTC10.8928571428571428No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACC10.8928571428571428No Hit
CACCTGGCAAGTTCAGGCCGGGCGCGGTGGCTCATGCCTGTTA10.8928571428571428No Hit
GAGGAAGAGGAAAAAGAAGTCGAAAATGAGGATGAAGACGATG10.8928571428571428No Hit
ACCATGCGCTGCTCAACCTCTCTCCTTCTGGCGCAAGAGAACA10.8928571428571428No Hit
GTGAAGGACCGTGTGGCCATCCTCGTGGATGACATGGCTGACA10.8928571428571428No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGCCT10.8928571428571428No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACCTGT10.8928571428571428No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCACCCTTGTATT10.8928571428571428No Hit
GCTGGAGAACTACTGCAACTAGACGCAGCCCGCAGGCAGCCCC10.8928571428571428No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGT10.8928571428571428No Hit
TCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAG10.8928571428571428No Hit
ACTATGGGTGTTAAATTTTCTTACTCTCTCTACAAGGTTTTTT10.8928571428571428No Hit
ACGCTCGAGGAAGCGCGCACGGACATTGCCGCGCTTGCCACGG10.8928571428571428No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers