FastQCFastQC Report
Fri 10 Feb 2017
ERR1631288.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631288.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381661
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.5958167064489167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21880.5732836208048504No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16430.4304867408511742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.18917311436064987No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4640.12157385742845091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTA250.005491800629.614
TGTTTAC358.858497E-426.42857214
TGATAAT358.858497E-426.42857210
ACCGTTA358.858497E-426.4285728
GATAATT400.00192883723.12511
CCTACGA400.00192883723.1252
TGCATTA1005.311449E-1022.219
ATCTTAC502.6975217E-422.21
GGTATCA11250.021.3777791
TATTAGA806.935079E-720.81252
AATATAG555.1344454E-420.181825
CTAAACT1101.7444108E-920.181824
TAAACTA1202.3646862E-1020.0416665
CCTGCAT1202.3646862E-1020.04166617
CACATGT656.886701E-519.92307728
GTGACAC656.886701E-519.92307724
GCATTAA1153.0322553E-919.30434820
GACACAT701.2164912E-418.526
CTATATG609.221414E-418.51
CCTGTTT500.007026484218.512
Produced by FastQC (version 0.11.5)