##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631288.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	381661
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75530903078911	34.0	31.0	34.0	31.0	34.0
2	32.91536468227039	34.0	33.0	34.0	31.0	34.0
3	33.01673998653255	34.0	33.0	34.0	31.0	34.0
4	36.38125456884513	37.0	37.0	37.0	35.0	37.0
5	36.35954682296593	37.0	37.0	37.0	35.0	37.0
6	36.4150594375637	37.0	37.0	37.0	35.0	37.0
7	36.41710837628157	37.0	37.0	37.0	35.0	37.0
8	36.40992399013785	37.0	37.0	37.0	35.0	37.0
9	38.254618627525474	39.0	39.0	39.0	37.0	39.0
10	38.20082481573962	39.0	39.0	39.0	37.0	39.0
11	38.268091316639634	39.0	39.0	39.0	37.0	39.0
12	38.04553255375844	39.0	39.0	39.0	37.0	39.0
13	38.18214069553871	39.0	39.0	39.0	37.0	39.0
14	39.663381901740024	41.0	40.0	41.0	37.0	41.0
15	39.65602982751709	41.0	40.0	41.0	37.0	41.0
16	39.615302585278556	41.0	39.0	41.0	37.0	41.0
17	39.61247284894186	41.0	39.0	41.0	37.0	41.0
18	39.622759464551	41.0	40.0	41.0	37.0	41.0
19	39.640573702841	41.0	40.0	41.0	37.0	41.0
20	39.59619924487962	41.0	40.0	41.0	37.0	41.0
21	39.573708081255354	41.0	40.0	41.0	37.0	41.0
22	39.5133429928654	41.0	39.0	41.0	37.0	41.0
23	39.451510633782334	41.0	39.0	41.0	37.0	41.0
24	39.41424981855626	41.0	39.0	41.0	36.0	41.0
25	39.38530266388235	41.0	39.0	41.0	36.0	41.0
26	39.26085976822363	41.0	39.0	41.0	36.0	41.0
27	39.18920193574927	41.0	39.0	41.0	35.0	41.0
28	39.117219207621424	41.0	39.0	41.0	35.0	41.0
29	39.017966205611785	40.0	39.0	41.0	35.0	41.0
30	38.90212518439139	40.0	38.0	41.0	35.0	41.0
31	38.78938901276263	40.0	38.0	41.0	35.0	41.0
32	38.67313139147044	40.0	38.0	41.0	35.0	41.0
33	38.55533313595049	40.0	38.0	41.0	35.0	41.0
34	38.48451374387218	40.0	38.0	41.0	35.0	41.0
35	38.34217014575762	40.0	38.0	41.0	35.0	41.0
36	38.246873534366884	40.0	37.0	41.0	34.0	41.0
37	38.12770495282463	40.0	37.0	41.0	34.0	41.0
38	37.98753343936111	40.0	37.0	41.0	34.0	41.0
39	37.85105892401896	40.0	37.0	41.0	34.0	41.0
40	37.71956788878088	40.0	36.0	41.0	33.0	41.0
41	37.54735485155675	40.0	36.0	41.0	33.0	41.0
42	37.38552275448631	40.0	35.0	41.0	33.0	41.0
43	36.41899224704646	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	1.0
15	2.0
16	1.0
17	4.0
18	10.0
19	13.0
20	31.0
21	82.0
22	147.0
23	210.0
24	327.0
25	505.0
26	696.0
27	1096.0
28	1552.0
29	2236.0
30	2885.0
31	3774.0
32	4771.0
33	6470.0
34	9097.0
35	13148.0
36	21650.0
37	44245.0
38	86972.0
39	181733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.417952056930105	18.206733200405594	11.847424808927293	25.527889933737008
2	20.4660680551589	19.463607756621716	32.56423894503237	27.506085243187016
3	20.30990853139304	22.30565868663552	28.742522814749215	28.641909967222222
4	14.416458584974624	16.343561432789834	34.07369367056104	35.1662863116745
5	15.168958840436932	36.028569856495686	32.66851996929212	16.13395133377526
6	32.59672851037963	34.89955746067845	16.127401018181057	16.37631301076086
7	30.5700608655325	29.694152664275364	20.04501376876338	19.690772701428756
8	29.74472110066263	30.69923308905023	18.258349687287932	21.297696122999206
9	27.931331731562825	13.229017374057081	19.200547082358426	39.639103812021666
10	20.4891251660505	24.271801415392193	29.066894442974263	26.172178975583044
11	37.016881473349386	21.964780263113077	19.57129494499045	21.447043318547088
12	23.375456229481138	26.279604151328012	25.22081113868066	25.124128480510187
13	32.47751276656509	18.378613481597544	22.98767754630418	26.156196205533185
14	23.92777884038453	19.693392827666436	23.835026371570585	32.543801960378445
15	28.876673278118542	24.717222875798157	20.778911127938144	25.62719271814516
16	26.520917777818536	24.15048957058751	22.979817167591136	26.348775484002818
17	24.39258923494934	25.787544443891306	23.881402605977556	25.938463715181797
18	24.3574795433644	23.5439303465641	25.22578937853226	26.872800731539247
19	25.76920356022753	24.30900720796728	25.97488346988558	23.94690576191961
20	25.855405713447272	23.381220507204038	25.950516295875136	24.812857483473554
21	26.717951270892232	23.511440781216837	24.777485779264847	24.993122168626083
22	27.317698166697674	23.082526116108273	24.176690832964333	25.423084884229723
23	25.638983286214728	23.678080809933423	25.193561825808768	25.48937407804308
24	25.081158410212208	24.091536730239664	24.024461498555	26.802843360993133
25	25.67121083893822	23.26069470027066	25.061769476053357	26.006324984737766
26	25.28107404214735	23.76768912726215	25.23155365625516	25.71968317433534
27	25.185963459719492	23.98804174385122	24.233547572322035	26.592447224107257
28	25.0696298547664	23.73126937255837	25.904926099339466	25.29417467333576
29	25.537322388192663	23.889000972066835	25.746932487207236	24.82674415253327
30	25.134608985460922	23.856773419343345	25.922742957755705	25.085874637440032
31	25.05259903422147	24.312413372076268	25.57714830700543	25.057839286696833
32	24.186909325291293	24.11564189162634	26.28641647954598	25.411032303536384
33	23.8381705230558	23.960006393108017	26.573844327819714	25.627978756016468
34	25.547016855272087	23.04794044977087	25.9895561768166	25.415486518140444
35	24.85477950327647	24.17721485821187	26.19445004860334	24.773555589908323
36	24.642287265400448	23.82690398023377	25.444831932002483	26.0859768223633
37	24.072147796080813	22.988725596799252	26.42528317014314	26.513843436976792
38	23.77109529137114	22.84435664110297	27.660934703834027	25.72361336369186
39	23.230301235913547	22.829421921548178	28.081202952358247	25.859073890180028
40	23.505676503493937	22.63186440322695	28.456667042218093	25.40579205106102
41	22.137970607423867	22.442429276242528	29.29458341302884	26.125016703304766
42	21.736305255187194	22.779901535655988	29.258425670948828	26.22536753820799
43	21.39097261706069	22.166791996038366	29.851098225912526	26.591137160988414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	54.0
2	56.0
3	148.0
4	240.0
5	240.0
6	309.0
7	378.0
8	373.0
9	368.0
10	571.0
11	774.0
12	774.0
13	1473.0
14	2172.0
15	3260.5
16	4349.0
17	3799.0
18	3249.0
19	3249.0
20	3637.5
21	4026.0
22	2788.5
23	1551.0
24	1489.5
25	1428.0
26	1428.0
27	1762.0
28	2096.0
29	3108.0
30	4120.0
31	5560.0
32	7000.0
33	7000.0
34	8929.5
35	10859.0
36	12710.5
37	14562.0
38	15805.5
39	17049.0
40	17049.0
41	19498.0
42	21947.0
43	23501.5
44	25056.0
45	25954.0
46	26852.0
47	26852.0
48	28218.5
49	29585.0
50	29405.0
51	29225.0
52	27591.0
53	25957.0
54	25957.0
55	24445.5
56	22934.0
57	21069.5
58	19205.0
59	19045.0
60	18885.0
61	18885.0
62	18514.5
63	18144.0
64	17152.5
65	16161.0
66	15315.0
67	14469.0
68	14469.0
69	13344.0
70	12219.0
71	10839.0
72	9459.0
73	8059.5
74	6660.0
75	6660.0
76	5568.5
77	4477.0
78	3629.0
79	2781.0
80	2164.0
81	1547.0
82	1547.0
83	1225.0
84	903.0
85	682.5
86	462.0
87	344.0
88	226.0
89	226.0
90	168.0
91	110.0
92	78.0
93	46.0
94	33.0
95	20.0
96	20.0
97	10.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	381661.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.26940317663602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.48536737815209	42.80244959460057
2	7.93291914327605	8.134320598485477
3	2.539837230419452	3.906478169082164
4	1.253438495670421	2.570521743665718
5	0.8420036459193043	2.1584512199417145
6	0.580143669793681	1.784617182421526
7	0.47035703790551064	1.6880447229342128
8	0.3481972929951621	1.4281493919665782
9	0.2737218225486798	1.26301990306427
>10	2.022029491975906	20.387876256302274
>50	0.1755169015062713	6.229852631313652
>100	0.07441506058014395	5.849909766946656
>500	5.132073143458202E-4	0.18997143218348783
>1k	0.0015396219430374608	1.6063373870916806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2274	0.5958167064489167	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2188	0.5732836208048504	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1643	0.4304867408511742	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	722	0.18917311436064987	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	464	0.12157385742845091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.6201262376821313E-4	0.0
10	0.0	0.0	0.0	5.240252475364263E-4	2.6201262376821313E-4
11	0.0	0.0	0.0	7.860378713046395E-4	2.6201262376821313E-4
12	0.0	0.0	0.0	0.0010480504950728525	2.6201262376821313E-4
13	0.0	0.0	0.0	0.0010480504950728525	2.6201262376821313E-4
14	0.0	0.0	0.0	0.0010480504950728525	2.6201262376821313E-4
15	0.0	0.0	0.0	0.001572075742609279	2.6201262376821313E-4
16	0.0	0.0	0.0	0.0018340883663774921	2.6201262376821313E-4
17	0.0	0.0	0.0	0.002882138861450345	2.6201262376821313E-4
18	0.0	0.0	0.0	0.003144151485218558	2.6201262376821313E-4
19	0.0	0.0	0.0	0.003406164108986771	2.6201262376821313E-4
20	0.0	0.0	0.0	0.0036681767327549843	2.6201262376821313E-4
21	0.0	0.0	0.0	0.004716227227827836	2.6201262376821313E-4
22	0.0	0.0	0.0	0.006288302970437116	2.6201262376821313E-4
23	0.0	0.0	0.0	0.009432454455655673	2.6201262376821313E-4
24	0.0	0.0	0.0	0.015982770049861	2.6201262376821313E-4
25	0.0	0.0	0.0	0.02096100990145705	2.6201262376821313E-4
26	0.0	0.0	0.0	0.02829736336696702	2.6201262376821313E-4
27	0.0	0.0	0.0	0.07886579975423216	2.6201262376821313E-4
28	0.0	0.0	0.0	0.2271649448070408	2.6201262376821313E-4
29	0.0	0.0	0.0	0.4163380591676907	2.6201262376821313E-4
30	0.0	0.0	0.0	0.6534594836779236	2.6201262376821313E-4
31	0.0	0.0	0.0	1.1929434760166744	2.6201262376821313E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTGTA	25	0.0054918006	29.6	14
TGTTTAC	35	8.858497E-4	26.428572	14
TGATAAT	35	8.858497E-4	26.428572	10
ACCGTTA	35	8.858497E-4	26.428572	8
GATAATT	40	0.001928837	23.125	11
CCTACGA	40	0.001928837	23.125	2
TGCATTA	100	5.311449E-10	22.2	19
ATCTTAC	50	2.6975217E-4	22.2	1
GGTATCA	1125	0.0	21.377779	1
TATTAGA	80	6.935079E-7	20.8125	2
AATATAG	55	5.1344454E-4	20.18182	5
CTAAACT	110	1.7444108E-9	20.18182	4
TAAACTA	120	2.3646862E-10	20.041666	5
CCTGCAT	120	2.3646862E-10	20.041666	17
CACATGT	65	6.886701E-5	19.923077	28
GTGACAC	65	6.886701E-5	19.923077	24
GCATTAA	115	3.0322553E-9	19.304348	20
GACACAT	70	1.2164912E-4	18.5	26
CTATATG	60	9.221414E-4	18.5	1
CCTGTTT	50	0.0070264842	18.5	12
>>END_MODULE