FastQCFastQC Report
Fri 10 Feb 2017
ERR1631287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386
Sequences flagged as poor quality0
Sequence length43
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT302.1645021645021645TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT100.7215007215007215No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC90.6493506493506493RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC70.5050505050505051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60.4329004329004329No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA50.36075036075036077No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC40.2886002886002886No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA40.2886002886002886No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT30.21645021645021645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30.21645021645021645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30.21645021645021645No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTT30.21645021645021645No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA30.21645021645021645ABI Solid3 Adapter B (100% over 21bp)
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA30.21645021645021645No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA30.21645021645021645No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT30.21645021645021645No Hit
GCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAAC20.1443001443001443No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTG20.1443001443001443No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA20.1443001443001443No Hit
GCGGAACAGCTCGCGGTCGATCAGCACCACCGGCTTGGTGACA20.1443001443001443No Hit
TTTTTCCCCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATT20.1443001443001443ABI Solid3 Adapter B (100% over 23bp)
GCTTAAGCTCGCACGTTACATGAAGTCGCTGACCCATTATACA20.1443001443001443No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC20.1443001443001443No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG20.1443001443001443No Hit
GTGCAGCCGCGCGCGGCTCGCTCCGGTCATGGCCTGTCTCTTA20.1443001443001443No Hit
CTCAAGCGTTGAGCGGAATGCGGCAATCAATGTCGTCGGAAGA20.1443001443001443No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT20.1443001443001443No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT20.1443001443001443No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG20.1443001443001443No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT20.1443001443001443No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC20.1443001443001443No Hit
CGCATGAAGGACCGGGGCCTGCTGCCCTGGGTCGGCCTGCCCA20.1443001443001443No Hit
CAGGGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTC20.1443001443001443No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAACTCAAA20.1443001443001443No Hit
ATACCATGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATA20.1443001443001443No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTC20.1443001443001443No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)