##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631287.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1386
Sequences flagged as poor quality	0
Sequence length	43
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.778499278499275	34.0	31.0	34.0	31.0	34.0
2	32.92712842712843	34.0	33.0	34.0	31.0	34.0
3	32.95238095238095	34.0	33.0	34.0	31.0	34.0
4	36.374458874458874	37.0	37.0	37.0	35.0	37.0
5	36.36291486291486	37.0	37.0	37.0	35.0	37.0
6	36.40692640692641	37.0	37.0	37.0	35.0	37.0
7	36.36435786435786	37.0	37.0	37.0	35.0	37.0
8	36.2950937950938	37.0	37.0	37.0	35.0	37.0
9	38.20995670995671	39.0	39.0	39.0	37.0	39.0
10	38.152958152958156	39.0	39.0	39.0	37.0	39.0
11	38.287157287157285	39.0	39.0	39.0	37.0	39.0
12	37.84487734487735	39.0	38.0	39.0	35.0	39.0
13	38.057720057720054	39.0	38.0	39.0	37.0	39.0
14	39.63347763347763	41.0	39.0	41.0	37.0	41.0
15	39.54978354978355	41.0	39.0	41.0	37.0	41.0
16	39.522366522366525	41.0	39.0	41.0	37.0	41.0
17	39.52813852813853	41.0	39.0	41.0	37.0	41.0
18	39.510822510822514	41.0	39.0	41.0	37.0	41.0
19	39.497835497835496	41.0	39.0	41.0	37.0	41.0
20	39.46103896103896	41.0	39.0	41.0	37.0	41.0
21	39.404761904761905	41.0	39.0	41.0	37.0	41.0
22	39.43145743145743	41.0	39.0	41.0	37.0	41.0
23	39.34199134199134	41.0	39.0	41.0	36.0	41.0
24	39.275613275613274	41.0	39.0	41.0	36.0	41.0
25	39.27994227994228	41.0	39.0	41.0	36.0	41.0
26	39.05699855699856	40.0	39.0	41.0	35.0	41.0
27	38.897546897546896	40.0	39.0	41.0	35.0	41.0
28	38.966089466089464	40.0	39.0	41.0	35.0	41.0
29	38.87518037518038	40.0	39.0	41.0	35.0	41.0
30	38.74386724386724	40.0	38.0	41.0	35.0	41.0
31	38.61904761904762	40.0	38.0	41.0	34.0	41.0
32	38.567821067821065	40.0	38.0	41.0	35.0	41.0
33	38.23593073593074	40.0	37.0	41.0	34.0	41.0
34	38.16305916305916	40.0	37.0	41.0	33.0	41.0
35	38.103896103896105	40.0	37.0	41.0	33.0	41.0
36	38.02958152958153	40.0	37.0	41.0	33.0	41.0
37	37.897546897546896	40.0	37.0	41.0	33.0	41.0
38	37.66378066378066	40.0	36.0	41.0	33.0	41.0
39	37.40836940836941	40.0	36.0	41.0	33.0	41.0
40	37.32106782106782	39.0	36.0	41.0	33.0	41.0
41	37.27489177489178	39.0	35.0	41.0	32.0	41.0
42	37.04906204906205	39.0	35.0	41.0	33.0	41.0
43	35.73737373737374	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	1.0
22	2.0
23	2.0
24	3.0
25	2.0
26	3.0
27	3.0
28	3.0
29	8.0
30	13.0
31	12.0
32	13.0
33	37.0
34	38.0
35	54.0
36	84.0
37	159.0
38	336.0
39	612.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91919191919192	14.285714285714285	13.492063492063492	30.303030303030305
2	22.582972582972584	17.532467532467532	29.292929292929294	30.59163059163059
3	21.645021645021643	18.326118326118326	30.663780663780667	29.365079365079367
4	15.295815295815295	16.08946608946609	28.28282828282828	40.33189033189033
5	17.316017316017316	32.82828282828283	29.653679653679653	20.2020202020202
6	36.65223665223665	29.797979797979796	13.636363636363635	19.913419913419915
7	31.38528138528138	27.705627705627705	16.883116883116884	24.025974025974026
8	34.12698412698413	26.984126984126984	16.161616161616163	22.727272727272727
9	26.334776334776333	11.832611832611832	14.43001443001443	47.4025974025974
10	21.428571428571427	21.645021645021643	22.582972582972584	34.34343434343434
11	38.88888888888889	20.2020202020202	16.233766233766232	24.675324675324674
12	22.87157287157287	24.81962481962482	22.7994227994228	29.509379509379507
13	36.36363636363637	13.78066378066378	19.985569985569985	29.87012987012987
14	25.82972582972583	18.253968253968253	18.253968253968253	37.66233766233766
15	33.4054834054834	22.150072150072152	15.873015873015872	28.57142857142857
16	31.818181818181817	21.5007215007215	19.047619047619047	27.633477633477632
17	25.901875901875904	28.13852813852814	19.552669552669553	26.406926406926406
18	27.417027417027416	18.326118326118326	21.572871572871573	32.683982683982684
19	30.303030303030305	23.160173160173162	20.12987012987013	26.406926406926406
20	27.92207792207792	20.851370851370852	23.160173160173162	28.066378066378068
21	33.4054834054834	18.181818181818183	19.480519480519483	28.93217893217893
22	31.24098124098124	19.76911976911977	21.06782106782107	27.92207792207792
23	29.22077922077922	19.480519480519483	18.181818181818183	33.116883116883116
24	29.58152958152958	22.366522366522368	19.76911976911977	28.28282828282828
25	31.313131313131315	19.76911976911977	19.264069264069263	29.653679653679653
26	30.23088023088023	20.346320346320347	22.005772005772005	27.417027417027416
27	29.365079365079367	20.707070707070706	20.562770562770563	29.365079365079367
28	27.633477633477632	19.624819624819626	21.356421356421357	31.38528138528138
29	26.47907647907648	19.19191919191919	24.314574314574315	30.014430014430015
30	28.354978354978357	17.243867243867246	26.406926406926406	27.994227994227995
31	28.066378066378068	20.49062049062049	22.51082251082251	28.93217893217893
32	29.365079365079367	18.75901875901876	24.963924963924963	26.911976911976907
33	25.396825396825395	18.253968253968253	27.633477633477632	28.715728715728716
34	27.84992784992785	21.21212121212121	21.572871572871573	29.365079365079367
35	25.82972582972583	19.336219336219337	27.200577200577204	27.633477633477632
36	25.541125541125542	18.181818181818183	25.46897546897547	30.808080808080806
37	26.83982683982684	18.83116883116883	23.232323232323232	31.096681096681095
38	26.118326118326117	15.584415584415584	27.994227994227995	30.303030303030305
39	26.984126984126984	15.656565656565657	27.344877344877343	30.014430014430015
40	24.53102453102453	15.584415584415584	29.148629148629148	30.735930735930733
41	20.923520923520925	18.614718614718615	28.86002886002886	31.601731601731604
42	22.294372294372295	14.502164502164502	34.77633477633478	28.42712842712843
43	18.181818181818183	19.11976911976912	33.04473304473304	29.653679653679653
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	1.0
11	2.0
12	2.0
13	2.5
14	3.0
15	6.5
16	10.0
17	7.0
18	4.0
19	4.0
20	5.5
21	7.0
22	3.5
23	0.0
24	1.0
25	2.0
26	2.0
27	1.5
28	1.0
29	4.0
30	7.0
31	5.0
32	3.0
33	3.0
34	7.5
35	12.0
36	11.0
37	10.0
38	14.0
39	18.0
40	18.0
41	23.0
42	28.0
43	38.0
44	48.0
45	52.5
46	57.0
47	57.0
48	80.0
49	103.0
50	118.0
51	133.0
52	112.0
53	91.0
54	91.0
55	106.5
56	122.0
57	124.0
58	126.0
59	110.5
60	95.0
61	95.0
62	98.0
63	101.0
64	96.5
65	92.0
66	85.0
67	78.0
68	78.0
69	80.0
70	82.0
71	73.5
72	65.0
73	53.0
74	41.0
75	41.0
76	32.5
77	24.0
78	17.0
79	10.0
80	7.0
81	4.0
82	4.0
83	3.5
84	3.0
85	2.5
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1386.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.56854256854257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.19407638347623	89.97113997113996
2	1.558846453624318	2.886002886002886
3	0.6235385814497272	1.7316017316017316
4	0.1558846453624318	0.5772005772005772
5	0.0779423226812159	0.36075036075036077
6	0.0779423226812159	0.4329004329004329
7	0.0779423226812159	0.5050505050505051
8	0.0	0.0
9	0.0779423226812159	0.6493506493506493
>10	0.1558846453624318	2.886002886002886
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	30	2.1645021645021645	TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	10	0.7215007215007215	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	9	0.6493506493506493	RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	7	0.5050505050505051	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.4329004329004329	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	5	0.36075036075036077	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	4	0.2886002886002886	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	4	0.2886002886002886	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	3	0.21645021645021645	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.21645021645021645	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.21645021645021645	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTT	3	0.21645021645021645	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	3	0.21645021645021645	ABI Solid3 Adapter B (100% over 21bp)
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	3	0.21645021645021645	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	3	0.21645021645021645	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	3	0.21645021645021645	No Hit
GCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAAC	2	0.1443001443001443	No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTG	2	0.1443001443001443	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2	0.1443001443001443	No Hit
GCGGAACAGCTCGCGGTCGATCAGCACCACCGGCTTGGTGACA	2	0.1443001443001443	No Hit
TTTTTCCCCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATT	2	0.1443001443001443	ABI Solid3 Adapter B (100% over 23bp)
GCTTAAGCTCGCACGTTACATGAAGTCGCTGACCCATTATACA	2	0.1443001443001443	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	2	0.1443001443001443	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	2	0.1443001443001443	No Hit
GTGCAGCCGCGCGCGGCTCGCTCCGGTCATGGCCTGTCTCTTA	2	0.1443001443001443	No Hit
CTCAAGCGTTGAGCGGAATGCGGCAATCAATGTCGTCGGAAGA	2	0.1443001443001443	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	2	0.1443001443001443	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	2	0.1443001443001443	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	2	0.1443001443001443	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	2	0.1443001443001443	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	2	0.1443001443001443	No Hit
CGCATGAAGGACCGGGGCCTGCTGCCCTGGGTCGGCCTGCCCA	2	0.1443001443001443	No Hit
CAGGGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTC	2	0.1443001443001443	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAACTCAAA	2	0.1443001443001443	No Hit
ATACCATGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATA	2	0.1443001443001443	No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTC	2	0.1443001443001443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.07215007215007214	0.0
25	0.0	0.0	0.0	0.07215007215007214	0.0
26	0.0	0.0	0.0	0.07215007215007214	0.0
27	0.0	0.0	0.0	0.07215007215007214	0.0
28	0.0	0.0	0.0	0.5772005772005772	0.0
29	0.0	0.0	0.0	0.8658008658008658	0.0
30	0.0	0.0	0.0	1.443001443001443	0.0
31	0.0	0.0	0.0	2.380952380952381	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE