FastQCFastQC Report
Fri 10 Feb 2017
ERR1631286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26809
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2781.0369651982543175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2490.9287925696594427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2010.7497482188817188No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.39165951732627097No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.19023462270133165No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA450.16785407885411616No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA330.12309299115968518No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.12309299115968518No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA270.10071244731246969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATG352.3030669E-531.7142851
TCTATGG352.3030669E-531.7142852
TTCCTCG303.5073407E-430.83333220
CTATGGG405.7297933E-527.753
TATGGGC405.7297933E-527.754
GCCTTAT358.644479E-426.4285726
GGGCAGT358.644479E-426.428577
TCGCCTT358.644479E-426.4285724
CCTTATT358.644479E-426.4285727
AGTCGGT451.2767295E-424.66666811
TGGGCAG451.2767295E-424.6666686
GGTGATT400.00188267423.12515
CAGTCGG400.00188267423.12510
CGCCTTA400.00188267423.12525
TCGGTGA400.00188267423.12513
TGATTCC400.00188267423.12517
GCAGTCG400.00188267423.1259
CTTATTG400.00188267423.12528
GTGATTC400.00188267423.12516
ATTCCTC400.00188267423.12519
Produced by FastQC (version 0.11.5)