##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631286.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26809
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72979223395129	34.0	31.0	34.0	31.0	34.0
2	32.890559140587115	34.0	33.0	34.0	31.0	34.0
3	32.97474728635906	34.0	33.0	34.0	31.0	34.0
4	36.33645417583647	37.0	37.0	37.0	35.0	37.0
5	36.32720355104629	37.0	37.0	37.0	35.0	37.0
6	36.37677645566787	37.0	37.0	37.0	35.0	37.0
7	36.357715692491325	37.0	37.0	37.0	35.0	37.0
8	36.37465030400239	37.0	37.0	37.0	35.0	37.0
9	38.18974971091797	39.0	39.0	39.0	37.0	39.0
10	38.168450893356706	39.0	39.0	39.0	37.0	39.0
11	38.24357491887053	39.0	39.0	39.0	37.0	39.0
12	38.00984743929278	39.0	38.0	39.0	37.0	39.0
13	38.149017121116046	39.0	39.0	39.0	37.0	39.0
14	39.59442724458204	41.0	39.0	41.0	37.0	41.0
15	39.575478384124736	41.0	39.0	41.0	37.0	41.0
16	39.52385392965049	41.0	39.0	41.0	37.0	41.0
17	39.54657018165541	41.0	39.0	41.0	37.0	41.0
18	39.54384721548734	41.0	39.0	41.0	37.0	41.0
19	39.5614159424074	41.0	40.0	41.0	37.0	41.0
20	39.49330448729904	41.0	39.0	41.0	37.0	41.0
21	39.47540005222127	41.0	39.0	41.0	37.0	41.0
22	39.43992689022343	41.0	39.0	41.0	36.0	41.0
23	39.33943824834944	41.0	39.0	41.0	36.0	41.0
24	39.26726099444217	41.0	39.0	41.0	36.0	41.0
25	39.21850870976165	41.0	39.0	41.0	35.0	41.0
26	39.032563691297696	41.0	39.0	41.0	35.0	41.0
27	38.918870528553846	40.0	39.0	41.0	35.0	41.0
28	38.78779514342199	40.0	38.0	41.0	35.0	41.0
29	38.678764593979636	40.0	38.0	41.0	35.0	41.0
30	38.53067999552389	40.0	38.0	41.0	35.0	41.0
31	38.37569472938193	40.0	37.0	41.0	35.0	41.0
32	38.19500913872207	40.0	37.0	41.0	35.0	41.0
33	38.03245178857846	40.0	37.0	41.0	34.0	41.0
34	37.873438024543994	40.0	36.0	41.0	34.0	41.0
35	37.6479540452833	40.0	36.0	41.0	33.0	41.0
36	37.50524077735089	40.0	35.0	41.0	33.0	41.0
37	37.31276810026483	40.0	35.0	41.0	33.0	41.0
38	37.102465589913834	40.0	35.0	41.0	33.0	41.0
39	36.87291581185423	39.0	35.0	41.0	32.0	41.0
40	36.64366444104592	39.0	35.0	41.0	32.0	41.0
41	36.3880413294043	39.0	35.0	41.0	31.0	41.0
42	36.149464731992985	39.0	35.0	41.0	31.0	41.0
43	35.09698235667127	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	3.0
20	3.0
21	7.0
22	9.0
23	22.0
24	48.0
25	42.0
26	85.0
27	106.0
28	161.0
29	214.0
30	269.0
31	387.0
32	424.0
33	526.0
34	828.0
35	1116.0
36	1782.0
37	3733.0
38	5968.0
39	11075.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77940243947928	17.25166921556194	12.368980566227759	24.599947778731025
2	20.280502816218434	20.093998284158303	31.120146219553135	28.50535268007013
3	22.634190010817264	21.19810511395427	27.44600693796859	28.721697937259876
4	16.334066917826103	15.782013502928121	29.695251594613747	38.188667984632026
5	16.7630273415644	35.8387108806744	29.064866276250513	18.333395501510687
6	34.29072326457533	30.08691111194002	16.21470401730762	19.40766160617703
7	30.82546905889813	29.665410869484127	18.04617852213809	21.462941549479652
8	32.2167928680667	28.732888209183482	17.669439367376626	21.380879555373195
9	28.934313103808424	12.54429482636428	18.6429930247305	39.8783990450968
10	22.30967212503264	24.003133276138612	26.48364355253833	27.203551046290425
11	37.35685777164385	21.421910552426425	18.04617852213809	23.175053153791637
12	23.68234548099519	26.304599201760603	24.245589167816778	25.76746614942743
13	34.76817486664926	17.374762206721623	22.99227871237271	24.864784214256407
14	25.469058898131223	19.213696892834495	23.391398410981388	31.925845798052894
15	30.84784960274535	23.201163788280056	20.545339251743815	25.40564735723078
16	26.699988809728076	23.09299115968518	22.96243798724309	27.24458204334365
17	24.73423104181432	25.383266813383564	23.59655339624753	26.28594874855459
18	24.30527061807602	22.246260584132195	25.815957327763066	27.63251147002872
19	26.03603267559402	24.156066992427917	26.177776119959717	23.63012421201835
20	25.96516095341117	22.361893394009474	26.524674549591552	25.148271102987803
21	28.02044089671379	21.75015852885225	25.33477563504793	24.894624939386027
22	28.277817150956768	21.93666306091238	24.230668805251966	25.554850982878886
23	26.06587340072364	22.943787534037078	24.78645231079115	26.203886754448135
24	25.461598716848822	23.95837218844418	25.077399380804955	25.502629713902046
25	26.38293110522586	22.522287291581186	24.81256294527957	26.282218657913386
26	26.013652131746802	23.115371703532396	24.778992129508747	26.091984035212057
27	25.909209593793133	22.772203364541756	24.77153194822634	26.54705509343877
28	25.476519079413627	23.376478048416576	25.655563430191354	25.491439441978443
29	24.958036480286474	23.507031220858668	25.890559140587115	25.644373158267747
30	24.37614234025887	23.9882129135738	26.722369353575292	24.91327539259204
31	25.01025774926331	23.779327837666457	25.513819985825652	25.696594427244584
32	23.764407475101645	24.025513819985825	26.905143794994217	25.30493490991831
33	24.74542131373792	23.369017867134172	26.207616845089337	25.67794397403857
34	25.45040844492521	23.00346898429632	25.4354880823604	26.110634488418068
35	24.07400499832146	23.697265843559997	26.222537207654145	26.006191950464398
36	24.100115632809878	22.932597262113468	26.41277183035548	26.554515274721176
37	24.036704091909435	22.533477563504793	26.77459062255213	26.655227722033647
38	22.921406990189862	22.104517139766497	28.852251109701964	26.121824760341678
39	23.20862396956246	22.014994964377635	29.273751352157856	25.502629713902046
40	22.787123727106568	22.212689768361372	29.008914916632474	25.991271587899583
41	21.101122757283004	22.186579133872954	30.105561565146033	26.606736543698013
42	21.32492819575516	22.07840650527808	29.46398597485919	27.132679324107578
43	20.284232906859636	22.552128016710807	30.21373419374091	26.949904882688646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	5.0
2	7.0
3	20.5
4	34.0
5	34.0
6	45.5
7	57.0
8	58.0
9	59.0
10	102.0
11	145.0
12	145.0
13	253.0
14	361.0
15	473.0
16	585.0
17	522.0
18	459.0
19	459.0
20	494.5
21	530.0
22	395.5
23	261.0
24	229.0
25	197.0
26	197.0
27	188.0
28	179.0
29	224.0
30	269.0
31	289.5
32	310.0
33	310.0
34	358.5
35	407.0
36	473.5
37	540.0
38	612.0
39	684.0
40	684.0
41	816.0
42	948.0
43	1065.0
44	1182.0
45	1294.5
46	1407.0
47	1407.0
48	1603.5
49	1800.0
50	1792.0
51	1784.0
52	1675.5
53	1567.0
54	1567.0
55	1547.5
56	1528.0
57	1562.0
58	1596.0
59	1652.0
60	1708.0
61	1708.0
62	1700.0
63	1692.0
64	1637.0
65	1582.0
66	1493.0
67	1404.0
68	1404.0
69	1288.5
70	1173.0
71	1040.0
72	907.0
73	764.5
74	622.0
75	622.0
76	512.5
77	403.0
78	307.0
79	211.0
80	166.5
81	122.0
82	122.0
83	85.5
84	49.0
85	38.0
86	27.0
87	17.5
88	8.0
89	8.0
90	5.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26809.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.45264649930994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.54838709677419	79.93957253161251
2	4.303985333275132	7.3557387444514895
3	0.986511851237505	2.5290014547353503
4	0.43650966868916147	1.4920362564810326
5	0.2007944475970143	0.8579208474765936
6	0.10476232048539874	0.5371330523331717
7	0.0873019337378323	0.5222126897683613
8	0.06984154699026583	0.4774516020739304
9	0.03928587018202453	0.3021373419374091
>10	0.2007944475970143	2.689395352307061
>50	0.0043650966868916145	0.19023462270133165
>100	0.017460386747566458	3.1071655041217503
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	278	1.0369651982543175	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.9287925696594427	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	201	0.7497482188817188	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.39165951732627097	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51	0.19023462270133165	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	45	0.16785407885411616	No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	33	0.12309299115968518	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.12309299115968518	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	27	0.10071244731246969	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.003730090641202581	0.0
22	0.0	0.0	0.0	0.007460181282405162	0.0
23	0.0	0.0	0.0	0.007460181282405162	0.0
24	0.0	0.0	0.0	0.026110634488418067	0.0
25	0.0	0.0	0.0	0.02984072512962065	0.0
26	0.0	0.0	0.0	0.041030997053228394	0.0
27	0.0	0.0	0.0	0.13055317244209033	0.0
28	0.0	0.0	0.0	0.4177701518146891	0.0
29	0.0	0.0	0.0	0.7609384908053266	0.0
30	0.0	0.0	0.0	1.104106829795964	0.0
31	0.0	0.0	0.0	1.8128240516244545	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTATG	35	2.3030669E-5	31.714285	1
TCTATGG	35	2.3030669E-5	31.714285	2
TTCCTCG	30	3.5073407E-4	30.833332	20
CTATGGG	40	5.7297933E-5	27.75	3
TATGGGC	40	5.7297933E-5	27.75	4
GCCTTAT	35	8.644479E-4	26.42857	26
GGGCAGT	35	8.644479E-4	26.42857	7
TCGCCTT	35	8.644479E-4	26.42857	24
CCTTATT	35	8.644479E-4	26.42857	27
AGTCGGT	45	1.2767295E-4	24.666668	11
TGGGCAG	45	1.2767295E-4	24.666668	6
GGTGATT	40	0.001882674	23.125	15
CAGTCGG	40	0.001882674	23.125	10
CGCCTTA	40	0.001882674	23.125	25
TCGGTGA	40	0.001882674	23.125	13
TGATTCC	40	0.001882674	23.125	17
GCAGTCG	40	0.001882674	23.125	9
CTTATTG	40	0.001882674	23.125	28
GTGATTC	40	0.001882674	23.125	16
ATTCCTC	40	0.001882674	23.125	19
>>END_MODULE