FastQCFastQC Report
Fri 10 Feb 2017
ERR1631284.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631284.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627560
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19180.30562814710943975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13740.21894320861750272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13440.21416278921537382No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA8060.12843393460386257No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA7980.12715915609662823No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC7520.11982917968003058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC251.2329871E-437.03
TATAGGT358.864891E-426.428575
TAAGGTA809.767973E-1025.43755
GGTAAAC451.3222774E-424.66666635
TAGCGAA751.3711542E-824.66666610
TCTAATA802.7208443E-823.1252
CTTGTAA652.6796224E-622.7692312
TTGGTAA502.7002086E-422.233
TTAGTCC603.7228165E-521.58333428
TAATACT1059.804353E-1021.1428594
GATACAC705.095835E-621.1428573
ACTTTCA705.095835E-621.1428578
CTAATAC806.949758E-720.81253
ATTGACT1251.8189894E-1120.7231
CGTTATA450.003823902220.5555552
GTAGCGA909.4611096E-820.5555559
ACGTATA450.003823902220.55555529
GCTAAGC450.003823902220.5555558
AATACTG909.4611096E-820.5555555
TAAACTA555.139518E-420.1818185
Produced by FastQC (version 0.11.5)