##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631284.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	627560
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.845031550768056	34.0	33.0	34.0	31.0	34.0
2	33.01408948945121	34.0	33.0	34.0	31.0	34.0
3	33.09238957231181	34.0	33.0	34.0	31.0	34.0
4	36.438079227484224	37.0	37.0	37.0	35.0	37.0
5	36.42960035693798	37.0	37.0	37.0	35.0	37.0
6	36.48056122123781	37.0	37.0	37.0	35.0	37.0
7	36.468868315380206	37.0	37.0	37.0	35.0	37.0
8	36.4553923130856	37.0	37.0	37.0	35.0	37.0
9	38.3097966728281	39.0	39.0	39.0	37.0	39.0
10	38.277052393396644	39.0	39.0	39.0	37.0	39.0
11	38.331726050098794	39.0	39.0	39.0	37.0	39.0
12	38.12621741347441	39.0	39.0	39.0	37.0	39.0
13	38.22872394671426	39.0	39.0	39.0	37.0	39.0
14	39.76660558352986	41.0	40.0	41.0	38.0	41.0
15	39.77204410733635	41.0	40.0	41.0	38.0	41.0
16	39.7277710497801	41.0	40.0	41.0	38.0	41.0
17	39.71439384281981	41.0	40.0	41.0	38.0	41.0
18	39.72312448212123	41.0	40.0	41.0	38.0	41.0
19	39.756413729364525	41.0	40.0	41.0	38.0	41.0
20	39.73875645356619	41.0	40.0	41.0	38.0	41.0
21	39.70892185607751	41.0	40.0	41.0	38.0	41.0
22	39.66620721524635	41.0	40.0	41.0	37.0	41.0
23	39.624950602332845	41.0	40.0	41.0	37.0	41.0
24	39.584503473771434	41.0	40.0	41.0	37.0	41.0
25	39.55037765313277	41.0	40.0	41.0	37.0	41.0
26	39.468548027280264	41.0	39.0	41.0	37.0	41.0
27	39.40120785263561	41.0	39.0	41.0	36.0	41.0
28	39.327402957486136	41.0	39.0	41.0	36.0	41.0
29	39.256150806297406	41.0	39.0	41.0	36.0	41.0
30	39.1697670342278	41.0	39.0	41.0	35.0	41.0
31	39.08384058894767	40.0	39.0	41.0	35.0	41.0
32	38.99037860921665	40.0	39.0	41.0	35.0	41.0
33	38.91303779718274	40.0	39.0	41.0	35.0	41.0
34	38.85747020205239	40.0	39.0	41.0	35.0	41.0
35	38.76706291031933	40.0	38.0	41.0	35.0	41.0
36	38.70832430365224	40.0	38.0	41.0	35.0	41.0
37	38.637653770157435	40.0	38.0	41.0	35.0	41.0
38	38.53459270826694	40.0	38.0	41.0	35.0	41.0
39	38.44129326279559	40.0	38.0	41.0	35.0	41.0
40	38.37122824909172	40.0	38.0	41.0	35.0	41.0
41	38.25544649117216	40.0	38.0	41.0	34.0	41.0
42	38.16783096436994	40.0	37.0	41.0	34.0	41.0
43	37.20810121741347	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	1.0
14	1.0
15	2.0
16	3.0
17	19.0
18	16.0
19	62.0
20	75.0
21	137.0
22	204.0
23	310.0
24	457.0
25	672.0
26	993.0
27	1369.0
28	2033.0
29	2850.0
30	3648.0
31	4750.0
32	6350.0
33	8588.0
34	12036.0
35	17670.0
36	30182.0
37	58911.0
38	133445.0
39	342771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52938364459175	15.74893237300019	13.641404805914972	27.080279176493082
2	19.994900885971063	19.296800305946842	34.00391994390974	26.70437886417235
3	20.214162789215372	19.938651284339347	29.50379246605902	30.34339346038626
4	15.901109057301294	16.16498820829881	30.870514373127666	37.06338836127223
5	16.455637707948245	34.183026324176176	32.179074510803744	17.182261457071835
6	34.77324877302569	31.43603798839952	16.41723500541781	17.373478233156987
7	31.624227165529987	28.914526101089937	18.46548537191663	20.995761361463448
8	29.08486837911913	31.946427433233477	18.772069602906495	20.196634584740902
9	28.082573777806108	14.311619606093442	17.212378099305244	40.393428516795204
10	19.482280578749442	24.96143795015616	28.217062910319328	27.339218560775063
11	36.370864937217156	21.803014851169607	18.740837529479254	23.08528268213398
12	23.859073236025242	24.723054369303334	25.525049397667154	25.892822997004274
13	32.418254828223596	17.972942826183953	23.71932564216967	25.88947670342278
14	24.990917203135954	18.90799286124036	22.50557715596915	33.595512779654534
15	29.280706227293006	23.863694308113963	20.287303206067946	26.56829625852508
16	27.317387978838674	24.04598763464848	21.993275543374338	26.643348843138504
17	25.176078781311745	25.644559882720376	22.83526674740264	26.344094588565238
18	24.937217158518706	22.999872522149275	24.871566065396138	27.191344253935878
19	27.002517687551787	24.305405060870676	24.29568487475301	24.396392376824526
20	27.593377525654915	21.948658295621133	25.271049780100707	25.186914398623237
21	28.413060105806615	22.206322901395882	23.00337816304417	26.37723882975333
22	28.095162215565043	22.180986678564597	21.972719739945184	27.75113136592517
23	26.37134297915737	23.48365096564472	22.808337051437313	27.336669003760594
24	26.91615144368666	23.34119446746128	23.717413474408822	26.02524061444324
25	26.733220727898527	22.486296131047233	23.21435400599146	27.566129135062784
26	27.59146535789407	22.367423035247626	23.284944865829562	26.756166741028746
27	27.424788068073173	21.98180253680923	22.434508254190835	28.158901140926762
28	25.851711390145958	22.542386385365543	24.17617438969979	27.429727834788704
29	26.212792402320094	23.04815475811078	24.59653260246032	26.1425202371088
30	25.645356619287398	23.606667091592836	24.9555420995602	25.792434189559565
31	26.22362801963159	23.169577410924852	24.098890942698706	26.507903626744856
32	24.292975970425136	22.964497418573522	26.00261329593983	26.739913315061507
33	25.440117279622665	22.04187647396265	25.827490598508508	26.690515647906178
34	26.248167505895854	21.927465102938363	24.95952578239531	26.86484160877048
35	25.359487539040092	22.265440754668877	25.273439989801773	27.101631716489262
36	24.2544139205813	21.649563388361273	26.486232392121867	27.60979029893556
37	24.767671617056536	20.450474854993946	26.664223341194464	28.11763018675505
38	23.98288609854038	19.558448594556697	28.684109885907322	27.7745554209956
39	23.650487602779016	19.181114156415322	29.803843457199314	27.364554783606348
40	22.850882784116262	19.200395181337242	30.073140416852574	27.875581617693925
41	20.900471668047675	19.880808209573587	31.98849512397221	27.230224998406527
42	21.2833832621582	19.305723755497482	30.989706163554082	28.421186818790233
43	19.67062910319332	20.424979284849258	32.1438587545414	27.760532857416027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	20.0
2	28.0
3	99.0
4	170.0
5	170.0
6	240.5
7	311.0
8	341.5
9	372.0
10	523.0
11	674.0
12	674.0
13	1210.5
14	1747.0
15	2846.0
16	3945.0
17	3512.0
18	3079.0
19	3079.0
20	3101.0
21	3123.0
22	2486.0
23	1849.0
24	1899.0
25	1949.0
26	1949.0
27	2153.5
28	2358.0
29	3807.0
30	5256.0
31	6376.0
32	7496.0
33	7496.0
34	8822.0
35	10148.0
36	11669.0
37	13190.0
38	17077.5
39	20965.0
40	20965.0
41	24918.0
42	28871.0
43	30709.0
44	32547.0
45	37831.0
46	43115.0
47	43115.0
48	49185.5
49	55256.0
50	59343.5
51	63431.0
52	63986.5
53	64542.0
54	64542.0
55	56831.5
56	49121.0
57	46045.5
58	42970.0
59	38812.0
60	34654.0
61	34654.0
62	33732.0
63	32810.0
64	30302.0
65	27794.0
66	25040.0
67	22286.0
68	22286.0
69	19908.0
70	17530.0
71	15572.0
72	13614.0
73	11496.5
74	9379.0
75	9379.0
76	7611.0
77	5843.0
78	4742.5
79	3642.0
80	2742.0
81	1842.0
82	1842.0
83	1407.0
84	972.0
85	693.5
86	415.0
87	293.0
88	171.0
89	171.0
90	114.5
91	58.0
92	38.0
93	18.0
94	11.5
95	5.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	627560.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.563229493132546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.69533514125585	45.365556731688606
2	8.025004222644826	8.59690285721765
3	2.5736025488590997	4.135513918459524
4	1.212658449558299	2.5981561132190985
5	0.7339032090815029	1.9655113006889486
6	0.48255437340671514	1.5508302387419195
7	0.32735165401060895	1.2273808238108739
8	0.24809263699347786	1.0630914280670463
9	0.18847383340461363	0.9085740472891575
>10	1.2264387539285995	13.067188907556659
>50	0.1658548251732283	6.2478962186760505
>100	0.11535127660827316	11.098406771714243
>500	0.004482562562497592	1.4329199466422204
>1k	8.965125124995183E-4	0.7420706962280311
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1918	0.30562814710943975	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1374	0.21894320861750272	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1344	0.21416278921537382	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	806	0.12843393460386257	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	798	0.12715915609662823	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	752	0.11982917968003058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.59347313404296E-4	0.0
10	0.0	0.0	0.0	1.59347313404296E-4	0.0
11	0.0	0.0	0.0	1.59347313404296E-4	0.0
12	0.0	0.0	0.0	1.59347313404296E-4	0.0
13	0.0	0.0	0.0	1.59347313404296E-4	0.0
14	0.0	0.0	0.0	1.59347313404296E-4	0.0
15	0.0	0.0	0.0	1.59347313404296E-4	0.0
16	0.0	0.0	0.0	9.56083880425776E-4	0.0
17	0.0	0.0	0.0	0.00159347313404296	1.59347313404296E-4
18	0.0	0.0	0.0	0.001912167760851552	1.59347313404296E-4
19	0.0	0.0	0.0	0.002549557014468736	1.59347313404296E-4
20	0.0	0.0	0.0	0.002708904327873032	1.59347313404296E-4
21	0.0	0.0	0.0	0.003346293581490216	1.59347313404296E-4
22	0.0	0.0	0.0	0.0039836828351074004	1.59347313404296E-4
23	0.0	0.0	0.0	0.010676269998087832	1.59347313404296E-4
24	0.0	0.0	0.0	0.02151188730957996	1.59347313404296E-4
25	0.0	0.0	0.0	0.025814264771495954	1.59347313404296E-4
26	0.0	0.0	0.0	0.03633118745617949	1.59347313404296E-4
27	0.0	0.0	0.0	0.09449295684874753	1.59347313404296E-4
28	0.0	0.0	0.0	0.3155076805405061	1.59347313404296E-4
29	0.0	0.0	0.0	0.654598763464848	1.59347313404296E-4
30	0.0	0.0	0.0	1.119096182038371	1.59347313404296E-4
31	0.0	0.0	0.0	2.28265026451654	1.59347313404296E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAC	25	1.2329871E-4	37.0	3
TATAGGT	35	8.864891E-4	26.42857	5
TAAGGTA	80	9.767973E-10	25.4375	5
GGTAAAC	45	1.3222774E-4	24.666666	35
TAGCGAA	75	1.3711542E-8	24.666666	10
TCTAATA	80	2.7208443E-8	23.125	2
CTTGTAA	65	2.6796224E-6	22.76923	12
TTGGTAA	50	2.7002086E-4	22.2	33
TTAGTCC	60	3.7228165E-5	21.583334	28
TAATACT	105	9.804353E-10	21.142859	4
GATACAC	70	5.095835E-6	21.142857	3
ACTTTCA	70	5.095835E-6	21.142857	8
CTAATAC	80	6.949758E-7	20.8125	3
ATTGACT	125	1.8189894E-11	20.72	31
CGTTATA	45	0.0038239022	20.555555	2
GTAGCGA	90	9.4611096E-8	20.555555	9
ACGTATA	45	0.0038239022	20.555555	29
GCTAAGC	45	0.0038239022	20.555555	8
AATACTG	90	9.4611096E-8	20.555555	5
TAAACTA	55	5.139518E-4	20.181818	5
>>END_MODULE