FastQCFastQC Report
Fri 10 Feb 2017
ERR1631283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681788
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.18973854017272093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28700.1706517111550326No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT23850.14181335578562815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22900.13616460576481695No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA19840.11796968464515147No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT19810.11779130306554692No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC17410.10352077669718181No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG16930.1006666714235088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.019.2170541
GTACTAA500.007036202618.51
ATACACA7100.017.97887437
TCGGTGA2700.017.1296313
TATCGCC1452.9831426E-916.58620833
TATACAC10300.016.52427137
CGGTGAT2650.016.05660414
ACTGCGT700.002593162715.8571438
GTTCTAA957.062782E-515.5789481
AAGACGG7000.015.3285725
CTTATAC29750.015.17310937
TACCCCG1101.4525851E-515.1363645
ATACCAT1353.9750194E-715.0740756
ACGGACC6850.014.8540148
ATCTAAC750.00410545814.79999920
GACGGAC6750.014.7999997
GCAGTCG2900.014.6724159
TACACAT3350.014.35820937
AGACGGA7100.014.3309866
CTTAAGC1304.4466196E-614.2307693
Produced by FastQC (version 0.11.5)