FastQCFastQC Report
Fri 10 Feb 2017
ERR1631282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302392
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.17626127675335326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4730.15641948199687822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4130.13657768724040317No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3450.11409031984973148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGT250.00549001829.68
GCTCTAA250.00549001829.61
TAGGGAT250.00549001829.65
CCGCTTA405.9258804E-527.7525
CGCTTAT405.9258804E-527.7526
GCAGTCG1005.293259E-1022.29
TCGGTGA1107.2759576E-1121.86363613
GGTATCA3650.021.794521
AGTCGGT1059.749783E-1021.14285711
CCGGAAC806.9252746E-720.812513
CAGTCGG1101.7389539E-920.18181810
GTCCTAT902.1415617E-618.51
TATGGGC1601.8189894E-1218.54
GGATACC1002.8575596E-718.525
TGGATAC1002.8575596E-718.524
TATACAC1800.018.537
CTCTATG1501.2732926E-1118.4999981
TCTATGG1552.0008883E-1117.9032252
ACCATAC1054.768426E-717.6190471
CGGAACC953.588637E-617.52631614
Produced by FastQC (version 0.11.5)